不同载体上人体脱落细胞富集方法的比较
Comparative Studies on Different Methods of Collecting Human Sloughed Epithelial Cells from Various Carriers
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摘要: 目的 对不同载体上人体脱落细胞的富集方法进行比较研究.方法 选取使用过的口罩、衣服、手套, 分别采取负压吸附、粘取、剪取和擦拭4种方法富集脱落细胞.用QIAGEN QIAamp DNA Investigator Kit提取DNA, 用AB Identifiler系统进行PCR扩增, 用AB 3130 XL自动遗传分析仪进行扩增产物的电泳分离, 用Genemapper ID v3.2软件进行STR分型.结果 口罩、手套类体积小的载体, 采取负压吸附、粘取、剪取、擦拭的方法, 均能得到完整的STR分型图谱;而对于大件的衣服载体, 采用负压吸附和直接剪取明显斑迹处可得到完整的STR分型图谱.结论 对于纺织类渗透性载体, 体积较大时可采用负压吸附法富集人体脱落细胞;对于有明显斑迹的, 可直接剪取可疑斑迹进行检测;体积较小的载体, 可采用脱落细胞富集器粘取, 富集尽可能多的人体脱落细胞.Abstract: Objective To comparatively study different methods of collecting human sloughed epithelial cells from various carriers.Methods The used face masks, clothes and gloves were selected.The sloughed epithelial cells were collected from the materials using the following different methods:adsorption by negative pressure, sticking with double-sided adhesive, scissoring and wiping.DNA was exacted using QIAGEN QIAamp DNA Investigator Kit.PCR was conducted using AB Identifiler kit.PCR product was separated by AB 3130 XL.STR typing was performed by Genemapper ID v3.2 software.Results To collect human sloughed epithelial cells from small carriers such as face masks and gloves, all the methods (adsorption by negative pressure, sticking with double-sided adhesive, scissoring and wiping) were useful.When big carriers such as clothing were mentioned, both methods (adsorption by negative pressure and scissoring) were useful.Conclusions For permeable carriers such as textile products, the method of adsorption by negative pressure can be used to collecting human sloughed epithelial cells from big carriers.The method of scissoring directly can be used to collecting human sloughed epithelial cells from carriers with clear spots.The method of sticking with double-sided adhesive can be used to collecting human sloughed epithelial cells from small carriers.
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Key words:
- Carrier /
- Sloughed epithelial cell /
- Collection method
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