槲皮素对裸鼠肝癌切除术后肿瘤复发的影响及机制

陈鹏; 阳锡军; 李春满; 刘锋; 阿永俊

槲皮素对裸鼠肝癌切除术后肿瘤复发的影响及机制

昆明医科大学第二附属医院

基金项目:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FZ025); 云南省教育厅科学研究基金资助项目 (2014Z065);

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出版历程
- 收稿日期: 2017-04-17

Influence of Quercetin on the Recurrence of Tumor After Liver Cancer Resection in Athymic Mice and its Mechanism

Funds:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FZ025); 云南省教育厅科学研究基金资助项目 (2014Z065);

摘要

摘要: 目的体内实验研究槲皮素能否抑制裸鼠肝癌切除术后的肿瘤复发, 探讨其可能的分子机制.方法建立模拟人肝癌切除术后肿瘤高复发的裸鼠模型, 实验组裸鼠槲皮素75 mg/ (kg·d) 灌胃, 观察各组裸鼠复发情况, Western-blot检测其P65、MMP-2、E-Cadherin蛋白的表达.结果槲皮素组校对照组肝内复发肿瘤体积减小[ (25.49±9.78) mm3vs (49.75±7.53) mm3, P<0.05], 复发灶数目减少[ (1.00±0.71) vs (2.20±0.84) , P<0.05].采用Western blot检测蛋白表达, 槲皮素组校对照组E-Cadherin表达增高[ (136.24±3.03) vs (144.70±5.02) , P<0.05], MMP-2蛋白表达下降[ (169.21±5.32) vs (158.17±4.80) , P<0.05], P65蛋白表达降低[ (170.07±6.61) vs (156.26±8.60) , P<0.05].结论槲皮素能够抑制裸鼠肝癌术后肿瘤的复发, 其机制可能与槲皮素上调E-Cadherin的表达, 下调P65蛋白和MMP-2有关.
- 槲皮素 /
- 肝癌切除术 /
- 复发 /
- P65 /
- MMP-2 /
- E-Cadherin
Abstract: Objective To study the impact of quercetin on tumor recurrence after hepatectomy in athymic mouse hepatoma and the mechanism.Methods We established high-recurrent athymic mouse model simulating post-operation human HCC.For experimental group, we administered the mice with quercetin solution 75 mg/ (kg.d) by gavage, and observed tumor recurrence of the mice.Then we detected the expression of MMP-2, TIMP-2 in recurrent tumor using Western-blot.Results Compared with control group, mice in quercetin group had smaller liver recurrent focus volumn [ (25.49±9.78) mm3 VS (49.75±7.53) mm3, P<0.05) ], less liver recurrent focus[ (1.00±0.71) vs (2.20±0.84) , P<0.05].Western-blot was used to detect the expression of P65, MMP-2 and E-Cadherin.Compared with control group, the P65 and MMP-2 expression of the recurrent tumor in quercetin group decreased [ (170.07±6.61) vs (156.26±8.60) , P<0.05], [ (169.21±5.32) vs (158.17±4.80) , P<0.05], and the E-Cadherin expression in quercetin group increased [ (136.24±3.03) vs (144.70±5.02) , P<0.05].Conclusions Quercetin could restrain tumor recurrence in athymic mice after hepatectomy.The mechanism may be that quercetin could decrease the expression of P65 and MMP-2 and increase the expression of E-Cadherin in recurrent tumor.
- Quercetin /
- Hepatectomy /
- Recurrence /
- P65 /
- MMP-2 /
- E-Cadherin

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参考文献(10)

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