正常足月儿母亲胎盘菌群检析

熊晶晶; 祁文瑾; 胡红卫; 曾洁; 黄蓉; 马润玫; 刘梅; 黄永坤

正常足月儿母亲胎盘菌群检析

1. 昆明医科大学第一附属医院儿科
2. 昆明医科大学第一附属医院产科

基金项目:

基金：国家自然科学基金资助项目 (81360068); “十二五”云南省特色学科群建设项目 (201009);

计量
- 文章访问数: 2711
- HTML全文浏览量: 841
- PDF下载量: 147
- 被引次数: 0
出版历程
- 收稿日期: 2017-05-21

The Detection and Analysis of the Placenta Microbiota of Normal Term Delivery

Funds:

基金：国家自然科学基金资助项目 (81360068); “十二五”云南省特色学科群建设项目 (201009);

摘要

摘要: 目的探讨正常足月儿母亲胎盘菌群的结构和组成.方法采集足月经阴道正常分娩的胎盘组织3份, 应用组织DNA提取试剂盒提取胎盘组织菌群的DNA, 用16Sr RNA基因的高通量技术对样本进行测序, 并对测序结果进行分析.结果在细菌门水平, 5个菌门被检测出, 即变形杆菌, 未分类细菌门, 放线菌门, 厚壁菌门和拟杆菌门.大部分序列属于变形杆菌门, 在胎盘组织中相对含量超过90%.在细菌属水平, 有24个细菌属被检测到, 主要属于变形杆菌门、放线菌门及厚壁菌门.胎盘中以变形杆菌门中的盐单胞菌属、Pelagibacterium和未分类的叶杆菌属为主.胎盘中也能检测到放线菌、大肠杆菌、志贺氏菌、单胞菌、多形杆状菌、链球菌、克雷伯氏菌、双歧杆菌、梭菌和葡萄球菌等.结论正常足月儿胎盘组织中有较丰富的细菌定植.
- 胎盘 /
- 菌群 /
- 高通量测序
Abstract: Objective To study the composition of the placenta microbiota of normal term delivery.Method Three placenta specimens were collected from full-term infant birth by normal spontaneous delivery.The flora DNA was extracted by Tissue Kit for 16SrRNA gene high-throughput nucleotide sequencing, then the sequencing results were analyzed.Results The placenta microbiota of normal spontaneous delivery at the bacterial phylum level showed five bacterial phylum were detected, including Proteobacteria, Unclassified Bacteria, Actinobacteria, Firmicutes and Bacteroidetes.Proteobacteria was the main parts of bacteria and more than 90 percent in the placenta microbiota.At the genus level, 24 bacterial genus were detected including Proteobacteria, Actinobacteria and Firmicutes.Halomona, Pelagibacterium and Unclassified Phyllobacteriacea of Proteobacteria genus was the main bacterial genus in the placenta microbiota.Actinotalea, Escherichia/Shigella, Sphingomonas, Bacteroides, Streptococcus, Klebsiella, Bifidobacterium, Clostridium and Staphylococcus.Conclusion There are abundant bacterial flora colonized in the normal term placenta.
- Placenta /
- Microbiota /
- High-throughput nucleotide sequencing

HTML全文

参考文献(13)

[1]马丁.布莱泽著, 傅贺译, 严青校.消失的微生物[M].湖南:湖南科学技术出版社, 2016:4-7, 96-114.
[2]	[2]GOLDENBERG R L, HAUTH J C, ANDREWS W W.In-trauterine infection and preterm delivery[J].N Engl J Med, 2000, 342 (20) :1500-1507.
[3]	[3]SATOKARI R, GRONROOS T, LAITINEN K, et al.Bifidobacterium and Lactobacillus DNA in the human placenta[J].Lett Appl Microbiol, 2009, 48 (1) :8-12.
[4]	[4]COLLADO M C, RAUTAVA S, AAKKO J, et al.Human gut colonisation may be initiated in utero by distinct microbial communities in the placenta and amniotic fluid[J].Sci Rep, 2016, 6 (23129) :1-12.
[5]	[5]AAGAARD K, MA J, ANTONY K M, et al.The placenta harbors a unique microbiome[J].Sci Transl Med, 2014, 6 (237) :237.
[6]	[6]GILL S R, POP M, DEBOY R T, et al.Metagenomic Analysis of the Human Distal Gut Microbiome[J].Science, 2006, 312 (5778) :1355-1359.
[7]	[7] QIN J, LI R, RAES J, et al.A human gut microbial gene catalogue established by metagenomic sequencing[J].Nature, 2010, 464 (7285) :59-65.
[8]	[8]DE GOFFAU MC, FUENTES S, VAN DEN BOGERT B, et al.Aberrant gut microbiota composition at the onset of type 1diabetes in young children[J].Diabetologia, 2014, 57 (8) :1569-1577.
[9]	[9]RINGEL Y, MAHARSHAK N, RINGEL-KULKA T, et al.High throughput sequencing reveals distinct microbial populations within the mucosal and luminal niches in healthy individuals[J].Gut Microbes, 2015, 6 (3) :173-181.
[10]	[10]LYNCH SV, PEDERSEN O.The Human Intestinal Microbiome in Health and Disease[J].N Engl J Med, 2016, 375 (24) :2369-2379.
[11]	[11]DOYLE R M, ALBER D G, JONES H E, et al.Term and preterm labour are associated with distinct microbial community structures in placental membranes which are independent of mode of delivery[J].Placenta, 2014, 35 (12) :1099-1101.
[12]	[12]CAO B, STOUT M J, LEE I, et al.Placental microbiome and its role in preterm birth[J].Neoreviews, 2014, 15 (12) :e537-e545.
[13]	[13]ZHENG J, XIAO X, ZHANG Q, et al.The placental microbiome varies in association with low virth weight in full-term neonates[J].Nutrients, 2015, 7 (8) :6924-6937.

相关文章(20)

[1]	王学丹, 刘一帆, 杨子寒, 张丹丹, 段怡, 杨志惠. 阑尾黏液性肿瘤临床病理及分子病理特征分析, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20240706
[2]	李丹, 万绪莲, 李律宇, 云宇, 罗光云, 刘韦兵, 林公府, 李宁, 黎勇坤, 段为钢. 尿酸酶缺失大鼠肠道菌群的变化, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230205
[3]	阳情姚, 曾洁, 张卫权, 宁月, 李檬, 李湛华, 刘梅, 熊晶晶, 黄永坤. 妊娠期糖尿病与正常足月儿母亲胎盘菌群DNA的差异, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20231006
[4]	何建萍, 吕梦欣, 秦茂华, 朱丽虹, 党峰博, 孙永波, 罗胜军, 罗兰, 唐健. 昆明地区357例Gilbert综合征基因变异谱分析, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20231015
[5]	刘四香, 黄永坤, 王明英, 胡红卫, 马敏, 凌昱. 功能性便秘患儿的肠道菌群分析及治疗干预, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220309
[6]	刘秋燕, 熊伟, 李程. 鼻咽癌同期放化疗抵抗细胞株与母细胞株lncRNA的差异表达谱分析, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20221108
[7]	何银东, 张峻, 黄桦, 柳汝明, 王茜. 人类胎盘的葡萄糖转运机制及GDM对胎盘葡萄糖转运的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220903
[8]	李馨怡, 李玥晓, 李黎仙, 李永平, 高鹰, 李艳红. 大学生牙龈炎龈上菌斑的微生物群落, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220207
[9]	孙岩波, 李为明, 李树民, 林岳瀛, 岑云云, 徐鹏远. 恶性梗阻性黄疸患者与健康人粪菌群的对比, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210516
[10]	郭珊珊, 刘继红. 妊娠期阴道菌群变化与胎膜早破的相关性, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20211107
[11]	梁睿, 淳于纬训, 沈焘, 孙乐, 李云峰. 肠道菌群和免疫在结直肠肿瘤中作用研究进展, 昆明医科大学学报.
[12]	戴海龙, 王南, 陈晓晴, 何臻一, 冯晓岚, 光雪峰. 冠心病患者肠道菌群的特征, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20201213
[13]	殷顺会, 詹烨明, 贾凤梅, 冉丽权, 张明珠. 高通量测序筛查特发性牙龈纤维瘤和正常牙龈外泌体miRNAs差异表达, 昆明医科大学学报.
[14]	王瑞涛, 贾庆安, 牟怡平, 耿智敏, 刘昌. 胆宁片调节肠道菌群移位消除胆道炎症及预防胆石形成的临床评价, 昆明医科大学学报.
[15]	朱艳玲, 牛兆仪, 靳晴, 李丽荣, 马雄涛, 马永红. 超声征象评分法诊断凶险性前置胎盘合并胎盘植入的应用, 昆明医科大学学报.
[16]	吴雪松, 孙锋, 鲍天昊, 柯阳, 王映华, 王华. Roux-en-Y胃旁路术对大鼠肠道厚壁菌群及APPL1的影响, 昆明医科大学学报.
[17]	杨佳. HAART使用的HIV感染者口腔真菌菌群特征, 昆明医科大学学报.
[18]	蔡艳. 高通量透析对维持性血液透析患者钙磷代谢的影响, 昆明医科大学学报.
[19]	师海英. TGF-β1、β3 在子痫前期患者胎盘组织中的表达, 昆明医科大学学报.
[20]	倪荣萍. 米非司酮治疗胎盘粘连及胎盘植入80例, 昆明医科大学学报.