Abstract: Objective To learn the prevalence and drug resistance of Streptococcus pneumoniae (SPN) in children in Yuxi from 2014 to 2016, and to provide the basis for clinical rational drug use.Methods We made a retrospective analysis of 326 children with SPN, a strain collected from January 2014 to December 2016 in Yuxi city children's Hospital, and statistical analysis.The infection condition was analyzed in the recent three years, in different season and in children with different age and different gender.The antimicrobial susceptibility test was performed by VITEK2 bacterial identification and susceptibility system of France bio Merieux company, according to the CLSI criteria of drug sensitivity analysis results.Results Children's SPN infection of Yuxi increased year by year, mainly in infants under one year of age in the 326 strains of SPN (62.3%) , most of them were boys (63.2%) , from the seasonal distribution, winter (10-12 months) than the susceptible (50.9%) .SPN to vancomycin and cefepime, amoxicillin/clavulanate, linezolid.Telithromycin sensitive rate was 100%, to ceftriaxone, cefotaxime resistance rate increased, the resistant rate of penicillin decreased, The resistance rate of clindamycin, clarithromycin, erythromycin and Azithomycinwas was extremely high.Conclusions The prevalence of SPN infection in children in Yuxi increased obviously, and the incidence rate was higher in winter.The infants under one year old were more susceptible to infection and the majority were boys.amoxicillin/clavulanate, and three or four generation cephalosporins can be used as the preferred drug for the treatment of SPN infection;penicillin, clindamycin, clarithromycin, erythromycin and azithromycin can not be used for the treatment of SPN infection;to vancomycin resistant strains.It is suggested that local epidemiological data should be taken for rational use of the drug according to the results of drug sensitivity.