Roux-en-Y胃旁路术对大鼠肠道厚壁菌群及APPL1的影响

吴雪松; 孙锋; 鲍天昊; 柯阳; 王映华; 王华

Roux-en-Y胃旁路术对大鼠肠道厚壁菌群及APPL1的影响

1. 昆明医科大学第二附属医院胃肠外科一病区
2. 云南省精神病医院
3. 昆明医科大学第二附属医院肝胆胰外科二病区

基金项目:

基金：国家自然科学基金资助项目 (81660399); 云南省应用基础研究计划项目 (201401UH00302);

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出版历程
- 收稿日期: 2017-05-15

Effects of Roux-en-Y Gastric Bypass on Intestinal Thickening Bacteria and APPL1 in Rats

Funds:

基金：国家自然科学基金资助项目 (81660399); 云南省应用基础研究计划项目 (201401UH00302);

摘要

摘要: 目的探讨Roux-en-Y胃旁路术对肠道厚壁菌群及APPL1的影响.方法使用Roux-en-Y胃旁路术作用于肥胖大鼠模型, 并观察术后以及术后粪菌移植对大鼠相关代谢指标的影响, 使用Western blot及q PCR检测不同分组的厚壁菌群及APPL1的表达.结果手术后大鼠的体重较术前明显下降 (P<0.05) , 粪菌移植后体重有所回升, S+T组的体重较S组明显升高 (P<0.01) ;手术后大鼠的血浆中TG表达较术前明显下降 (P<0.01) , S+T组的血浆中TG表达较S组明显上升 (P<0.05) ;同时在骨骼肌中发现S组的TG表达明显低于C组, 在S+T组中的TG表达明显高于S组 (P<0.05) ;同时发现, 在S组中TCH的表达明显较C组低, 而S+T组的TCH表达较S组升高 (P<0.05) ;FINS在S组中的表达较C组明显下降, FINS在S+T组的表达较S组明显升高 (P<0.05) ;FGB在S组中的表达较C组明显下降, S+T组的FGB表达较S组明显上升 (P<0.05) ;ISI在S组的表达较C组明显上升 (P<0.05) , ISI在S+T组的表达组较S组明显下降 (P<0.01) .厚壁菌群在S的表达较C组明显降低 (P<0.05) .Western blot检测以及m RNA的检测发现APPL1在S组的表达较C组明显升高 (P<0.01) , S+T组中APPL1的表达较S组明显降低 (P<0.05) .结论 Roux-en-Y胃旁路术可能是通过影响厚壁菌群的表达量进而调节体重的.
- Roux-en-Y胃旁路术 /
- 厚壁菌群 /
- APPL1 /
- 体重
Abstract: Objective To investigate the effect of Roux-en-Y gastric bypass on intestinal thick-walled bacteria and APPL1.Methods The effect of postoperative and postoperative fecal transplantation on the metabolic index of rats was observed by Roux-en-Y gastric bypass.The thick-walled bacteria and APPL1 expression were detected by Western blot and qPCR.Results The body weight of the rats in the S+T group was significantly higher than that in the S group (P<0.05) .The body weight of the rats in the S+T group was significantly higher than that in the control group (P<0.05) The expression of TG in S+T group was significantly higher than that in S group (P<0.05) .The expression of TG in S group was significantly lower than that in group C (P<0.05) The expression of TCH in the T group was significantly higher than that in the S group (P<0.05) .The expression of TCH in the S group was significantly higher than that in the S group (P<0.05) The expression of FINS in S group was significantly higher than that in group C (P<0.05) .The expression of FINS in S group was significantly higher than that in group S (P<0.05) .The expression of ISI in S group was significantly higher than that in group S (P<0.05) .The expression of ISI in S group was significantly higher than that in group S (P<0.05) .The expression of S thick-walled bacteria in S was significantly lower than that in group C (P <0.05) .Western blot and mRNA detection showed that the expression of APPL1 in S group was significantly higher than that in group C (P<0.01) .The expression of APPL1 in S+T group was significantly lower than that in S group (P <0.05) .Conclus ion Roux-en-Y gastric bypass may be mediated by affecting the expression of thick-walled flora.
- Roux-en-Y gastric bypass /
- Firmicutes /
- APPL1 /
- Body weight

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参考文献(17)

[1]	[1]MONGRAW-CHAFFIN ML, SAE P, HUXLEY R R, et al.The sex-specific association between BMI and coronary heart disease:a systematic review and meta-analysis of 95cohorts with 1·2 million participants[J].Lancet Diabetes Endocrinol, 2015, 3 (6) :437-449.
[2]	[2]HAO Z, TOWNSEND R L, MUMPHREY M B, et al.RYGB produces more sustained body weight loss and improvement of glycemic control compared with VSG in the diet-Induced obese mouse Model[J].Obes Surg, 2017, (7) :1-10.
[3]	[3]TORLONI MR, BETRN A P, HORTA B L, et al.Prepregnancy BMI and the risk of gestational diabetes:a systematic review of the literature with meta-analysis[J].Obes Rev, 2009, 10 (2) :194-203.
[4]	[4]MORRIS C, OGRADA C, RYAN M, et al.The relationship between BMI and metabolomic profiles:a focus on amino acids[J].Proc Nutr Soc, 2012, 71 (4) :634-638.
[5]	[5]STENMAN L K, BURCELIN R, LAHTINEN S.Establishing a causal link between gut microbes, body weight gain and glucose metabolism in humans-towards treatment with probiotics[J].Benef Microbes, 2016, 7 (1) :11-22.
[6]	[6]LI M, GU D, XU N, et al.Gut carbohydrate metabolism instead of fat metabolism regulated by gut microbes mediates high-fat diet-induced obesity[J].Benef Microbes, 2014, 5 (3) :335-344.
[7]	[7]SANZ Y, RASTMANESH R, AGOSTONI C, et al.Understanding the role of gut microbes and probiotics in obesity:how far are we[J].Pharmacol Res, 2013, 69 (1) :144-155.
[8]	[8]SANZ Y, SANTACRUZ A, DE PALMA G.Insights into the roles of gut microbes in obesity[J].Interdiscip Perspect Infect Dis, 2008, 2008:829101.
[9]	[9]ZHAO S, LIU W, WANG J, et al.Akkermansia muciniphila improves metabolic profiles by reducing inflammation in chow diet-fed mice[J].J Mol Endocrinol, 2017, 58 (1) :1-14.
[10]	[10]BORAO F, GORCEY S, CAPUANO A.Prospective single-site case series utilizing an endolumenal tissue anchoring system for revision of post-RYGB stomal and pouch dilatation[J].Surg Endosc, 2010, 24 (9) :2308-2313.
[11]	[11]HUERTA S, LI Z, ANTHONY T, et al.Feasibility of a supervised inpatient low-calorie diet program for massive weight loss prior to RYGB in superobese patients[J].Obes Surg, 2010, 20 (2) :173-180.
[12]	[12]HANDISURYA A, RIEDL M, VILA G, et al.Serum vaspin concentrations in relation to insulin sensitivity following RYGB-induced weight loss[J].Obes Surg, 2010, 20 (2) :198-203.
[13]	[13]MORROW J, GLUCK M, LORENCE M, et al.Night eating status and influence on body weight, body image, hunger, and cortisol pre-and post-Roux-en-Y Gastric Bypass (RYGB) surgery[J].Eat Weight Disord, 2008, 13 (4) :e96-99.
[14]	[14]JIANG X, ZHOU Y, WU K K, et al.APPL1 prevents pancreatic beta cell death and inflammation by dampening NFκB activation in a mouse model of type 1 diabetes[J].Diabetologia, 2017, 60 (3) :464-474.
[15]	[15]BARBIERI M, MARFELLA R, ESPOSITO A, et al.Incretin treatment and atherosclerotic plaque stability:Role of adiponectin/APPL1 signaling pathway[J].J Diabetes Complications, 2017, 31 (2) :295-303.
[16]	[16]DING Y, CAO Y, WANG B, et al.APPL1-Mediating Leptin Signaling Contributes to Proliferation and Migration of Cancer Cells[J].PLo S One, 2016, 11 (11) :e0166172.
[17]童俊露, 王佑民.生活方式对高脂肥胖大鼠APPL1和GLUT4的影响[J].安徽医科大学学报, 2012, 47 (12) :1393-1396.

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