基因沉默策略及其应用研究进展
Advances and Applications in Gene Silencing
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摘要: 基因沉默 (Gene silencing) 是生物体中特定基因由于各种原因不表达或表达减少的现象, 是通过表观遗传控制基因表达的重要机制, 通过基因沉默技术探索疑难疾病的治疗方法是当下的研究热点.随着基因工程的迅速发展, 基因沉默技术不断有新技术出现取代已有的技术.对基因沉默技术所包括的核酶技术、反义寡核酸技术、基因敲除、RNA干扰技术及其应用进行介绍.Abstract: Gene silencing is a phenomenon in which the specific genes in organisms are not expressed or reduced for various reasons. It is an important mechanism to control gene expression by epigenetic control. At present, scientists intend to explore the treatment of difficult diseases through gene silencing technology, and the research has become a hot spot. With the rapid development of gene engineering, gene silencing technology has been replaced by new technologies. This paper aims to introduce the ribozyme technology, antisense oligonucleic acid technology, gene knockout, RNA interference technology and their applications in gene silencing technology.
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[1] [1]CECH T R, BASS B L.Biological catalysis by RNA[J].Annual Review of Biochemistry, 1986, 55 (1) :599. [2] [2]ALTMAN S.Enzymatic cleavage of RNA by RNA (Nobel Lecture) [J].Angewandte Chemie International Edition in English, 1990, 10 (4) :317-337. [3] [3]KILPATRICK M W, PHYLACTOU L A, GODFREY M, et al.Delivery of a hammerhead ribozyme specifically down-regulates the production of fibrillin-1 by cultured dermal fibroblasts[J].Human Molecular Genetics, 1996, 5 (12) :1939. [4] [4]LIEBER A, KAY M A.Adenovirus-mediated expression of ribozymes in mice[J].Journal of Virology, 1996, 70 (5) :3153. [5] [5]CORBEAU P, WONG-STAAL F.Anti-HIV Effects of HIV Vectors[J].Virology, 1998, 243 (2) :268. [6] [6]LIMA W F, WU H, CROOKE S T.Human RNases H[J].Methods in Enzymology, 2001, 341 (341) :430-440. [7] [7]PAN W H, CLAWSON G A.Identifying accessible sites in RNA:the first step in designing antisense reagents[J].Current Medicinal Chemistry, 2006, 13 (25) :3083-3103. [8] [8]VICKERS T A, KOOS, BENNETTCT, et al.Efficient reduction oftarget RNAs by small interfering RNA and RNase H-dependentantisense agents[J].J Biol Chem, 2003, 278 (9) :7108-7118. [9] [9]BALDOCCHIRA, GLYNNERJ, CHINK, et al.Design considerations for array CGH to oligonucleotide arrays[J].Cytometry A, 2005, 67 (2) :129-136. [10] [10]HAHNKEK, JACOBSENM, GRUETZKAUA, et al.Striptease on glass:validation of an improved stripping procedure for in situ microarrays[J].Journal of Biotechnology, 2007, 128 (1) :1-13. [11] [11]COBAUGH C W, CANNONE J J, DOSHI K J, et al.Evaluation of the suitability of free-energy minimization using nearest-neighbor energy parameters for RNA secondary structure prediction[J].Bmc Bioinformatics, 2004, 5 (1) :1-22. [12] 张剑, 杨晓梅, 高建刚.小鼠基因敲除的研究进展[J].山东大学学报 (理学版) , 2011, 46 (10) :183-196. [13] [13]JASON MORTON, M.WAYNE DAVIS, ERIK M.JORGENSEN, et al.Induction and repair of zinc-finger nuclease-targeted double-strand breaks in Caenorhabditis elegans somatic cells[J].Proc Natl Acad Sci USA, 2006, 103 (44) :16370-16375. [14] [14]PA GAMMAGE, J RORBACH, AI VINCENT, et al.Mitochondrially targeted ZFNs for selective degradation of pathogenic mitochondrial genomes bearing large-scale deletions or point mutations[J].Embo Molecular Medicine, 2014, 6 (4) :6863-6869. [15] [15]CL RAMIREZ, MT CERTO, C MUSSOLINO, et al.Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects[J].Nucleic Acids Research, 2012, 40 (12) :5560-5568. [16] [16]PORTEUS M H, CARROLL D.Gene targeting using zinc finger nucleases[J].Nat Biotechnol, 2005, 23 (8) :967-973. [17] [17]GEURTS A M, COST G J, FREYVERT Y, et al.Knockout rats via embryo microinjection of zinc-finger nucleases[J].Science, 2009, 325 (5939) :433. [18] [18]CERMAK1 T, DOYLE E L, CHRISTIAN M, et al.Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting[J].Nucl Acids Res, 2011, 39 (12) :82. [19] [19]VM BEDELL, Y WANG, JM CAMPBELl, et al.In vivo genome editing using a high-efficiency TALEN system[J].Nature, 2012, 491 (7422) :114-118. [20] [20]DING Q, LEE Y K, SCHAEFER E K, et al.A TALEN genome-editing system for generating human stem cell-based disease models[J].Cell Stem Cell, 2012, 12 (2) :238-251. [21] [21]RODOLPHEBARRANGOU.RNA-mediated programmable DNA cleavage[J].Nat Biotechnol, 2012, 30 (9) :836-838. [22] [22]MUSSOLINO C, CATHOMEN T.RNA guides genome engineering[J].Nat Biotechnol, 2013, 31 (3) :208-209. [23] [23]CONG L, ANN RAN F, COX D, et al.Multiplex genome engineering using CRISPR/cas systems[J].Science, 2013, 339 (6121) :819-823. [24]张中华, 侯永泰.si RNA制备技术的研究进展[J].生命科学, 2004, 16 (4) :231-234. [25] [25]SOMAYEH S M, EHSAN A, VAHID S, et al.Potential si RNA molecules for nucleoprotein and M2/L overlapping region of respiratory syncytial virus:In silico design[J].Jundishapur Journal of Microbiology, 2016, 9 (4) :e34304. [26] [26]FAKHR E, ZARE F, TEIMOORITOOLABI L.Precise and efficient si RNA design:a key point in competent gene silencing[J].Cancer Gene Therapy, 2016, 23 (4) :73. [27]李泽豪, 任小元, 王世兵, 等.介导si RNA传递的非病毒载体及其研究进展[J].生命科学, 2014, 26 (4) :392-399. [28] [28]AMSALEM O, NASSAR T, BENHAMRON S, et al.Solid nano-in-nanoparticles for potential delivery of si RNA[J].Journal of Controlled Release, 2017, 10 (257) :144-155. [29] [29]DAHLMAN J E, CBARNES, OFKHAN, et al.In vivo endothelial si RNA delivery using polymeric nanoparticles with low molecular weight[J].Nature Nanotechnology, 2014, 9 (8) :648-655. [30] [30]HUSSER L, ALVES M P, RUGGLI N, et al.Identification of the role of RIG-I, MDA-5 and TLR3 in sensing RNA virusesin porcine epithelial cells using lentivirus-driven, RNA interference[J].Virus Res, 2011, 159 (1) :9-16. [31] [31]SUMIMOTO H, Y KAWAKAMI.Lentiviral vector-mediated RNAi and its use for cancer research[J].Future Oncology, 2016, 3 (6) :655-664. [32] [32]COHEN Z R, RAMISHETTI S, PESHES-YALOZ N, et al.Localized RNAi therapeutics of chemoresistant grade IV glioma using hyaluronangrafted lipid-based nanoparticles[J].Acs Nano, 2015, 9 (2) :1581-1591.
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