Abstract: Objective To investigate the status of psychological quality of college students ' innovation and entrepreneurship, identify the elements of the psychological quality and problems and countermeasures against the psychological quality training in medical schools. Methods A questionair was made and administered to Kunming Medical University students and Chi-square analysis was used to analyze the gender, major, grade, and entrepreneurial intention differences between different groups of students so as to analyze the psychological factors affecting College students ' undertaking intention. Results A total of 1150 questionnaires, 1099 was recovered, the effective recovery rate was 95.6%. Psychological quality score was (76.9±9.0) , good mental qualities only was8.2%, entrepreneurial environment, factors of psychological quality of good-willed persons were only 0.5% and15.5%. Female entrepreneurial intentions were better than those of men (P<0.001) . Science students' undertaking intention was superior to liberal arts students (P =0.025) , knowledge of entrepreneurship difference between knowledge of business groups and P<0.005, there was a statistically significant difference, about the environment worse group entrepreneurial intention superior knowledge of business groups. Conclusions As students' psychological qualities are poor, have poor psychological quality, in teaching we should strengthen the cultivation of medical students' entrepreneurial ability and correct understanding of entrepreneurship, create a good business environment, increase the entrepreneurship policy advocacy efforts, and create a good business environment and atmosphere so as to strengthen the entrepreneurship education, improve entrepreneurship courses so that students fully understand the cognitive and psychological qualities, stimulate the entrepreneurial motivation, lay the Foundation for entrepreneurship.