有氧运动对2型糖尿病大鼠AGE-RAGE轴及NF-κB通路的影响
有氧运动对2型糖尿病大鼠AGE-RAGE轴及NF-κB通路的影响
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摘要:
目的 研究有氧运动对2型糖尿病 (T2DM) 大鼠AGE-RAGE轴及NF-κB通路的影响, 探讨T2DM运动康复作用.方法 8周龄雄性Wister大鼠高糖高脂饮食后, 应用链脲佐菌素诱导建立T2DM大鼠.将30只造模成功的T2DM大鼠随机分成3组 (n=10) :T2DM组、T2DM加低强度运动组 (T2DML) 和T2DM加中强度运动组 (T2DMM) .运动组实施运动方案.采用ELISA法分别测定实验大鼠血浆、骨骼肌和心肌的晚期糖基化终产物 (AGEs) 及其受体 (RAGE) 和核因子κB (NF-κB) 水平.结果 与T2DM组相比, T2DML组血、骨骼肌和心肌的NF-κB水平显著下降 (P<0.05) ;T2DMM组血、骨骼肌和心肌的AGEs、RAGE和NF-κB水平均显著下降 (P<0.05) ;与T2DML组相比, T2DMM组血、骨骼肌和心肌的NF-κB水平均显著下降 (P<0.05) .结论 中强度有氧运动能抑制AGE-RAGE轴和NF-κB通路, 降低T2DM氧化应激和炎症反应, 减轻组织损伤, 对T2DM及其并发症具有防治作用.
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关键词:
- 2型糖尿病 /
- 有氧运动 /
- 晚期糖基化终产物 /
- 晚期糖基化终产物受体 /
- 核因子κB
Abstract:目的 研究有氧运动对2型糖尿病 (T2DM) 大鼠AGE-RAGE轴及NF-κB通路的影响, 探讨T2DM运动康复作用.方法 8周龄雄性Wister大鼠高糖高脂饮食后, 应用链脲佐菌素诱导建立T2DM大鼠.将30只造模成功的T2DM大鼠随机分成3组 (n=10) :T2DM组、T2DM加低强度运动组 (T2DML) 和T2DM加中强度运动组 (T2DMM) .运动组实施运动方案.采用ELISA法分别测定实验大鼠血浆、骨骼肌和心肌的晚期糖基化终产物 (AGEs) 及其受体 (RAGE) 和核因子κB (NF-κB) 水平.结果 与T2DM组相比, T2DML组血、骨骼肌和心肌的NF-κB水平显著下降 (P<0.05) ;T2DMM组血、骨骼肌和心肌的AGEs、RAGE和NF-κB水平均显著下降 (P<0.05) ;与T2DML组相比, T2DMM组血、骨骼肌和心肌的NF-κB水平均显著下降 (P<0.05) .结论 中强度有氧运动能抑制AGE-RAGE轴和NF-κB通路, 降低T2DM氧化应激和炎症反应, 减轻组织损伤, 对T2DM及其并发症具有防治作用.
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