子宫内膜异位症SCID小鼠动物模型的构建
The Establishment of SCID Mice Model on Endometriosis
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摘要: 目的 构建子宫内膜异位症 (内异症) 裸鼠及SCID小鼠动物模型, 比较在位及异位来源的人子宫内膜组织对移植效果的影响, 为内异症的药物治疗研究提供有利的动物模型基础.方法 取性成熟BALB/c-nu/nu裸鼠及SCID小鼠各30只, 通过皮下移植法, 将在位及异位子宫内膜移植到下腹部皮下, 定期观察异位病灶的大小, 术后6周取出病灶, 病理学检查.结果 裸鼠及SCID小鼠均可建立成功的内异症动物模型.其中SCID小鼠的建模成功率较裸鼠更高 (86.7%vs 66.7%) .在位子宫内膜组种植的成功率明显高于异位子宫内膜组 (100%vs84.2%) .结论 SCID小鼠在位内膜皮下种植方法可成功建立内异症动物模型.Abstract: Objective To set up a favorable animal model for the drug treatment research of endometriosis by establishing the animal model of endometriosis in SCID and nude mice so as to compare the influences on implantation of human endometrial tissue derived from the eutopic and ectopic sources. Methods Eutopic and ectopic endometrium were transplanted to the lower abdominal parts subcutaneously of 30 sexually matured BALB/c-nu/nu nude mice and SCID mice respectively. The ectopic lesion sizes were under the regular observation before they were removed 6 weeks after the operation for pathological examinations. Results Nude mice and SCID mice were able to be used to establish a successful animal models of endometriosis. The success rate of SCID mice was higher than that of nude mice. The success rate of the eutopic endometrium group was significantly higher than that of the ectopic endometrium group. Nude and SCID mice endometriosis implantation models were successfully established. The modeling success rate of SCID mice is higher than that of the nude mice.The success rate of transplantation was higher in the ectopic endometrium than in the eutopic endometrium.Conclusion The SCID mice endometriosis endometriosis model provides a favorable animal model of endometriosis.
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Key words:
- Endometriosis /
- SCID mice /
- Nude /
- Animal model /
- Endometrium
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[1]冯雪, 黄薇.子宫内膜异位症动物模型研究进展[J].中国比较医学杂志, 2014, 24 (12) :67-70. [2]崔阳阳, 孙伟伟, 赵瑞华.子宫内膜异位症动物模型研究进展[J].中国实验动物学报, 2013, 21 (5) :86-89. [3]赖秋红, 杨兆荣, 钱静.子宫内膜异位症动物模型研究进展[J].中国实验动物学报, 2009, 17 (1) :50-52. [4]张新艳, 霍晓溪, 刘海防, 等.内皮抑素基因抗血管生成治疗子宫内膜异位症的实验研究[J].解放军医学杂志, 2008, 33 (7) :852-856. [5] [5]BOEHM T, FOLKMAN J, BROW DER T, et al.Antiangiogenic therapy of experimental cancer does not induce acquired drug resistance[J].Nature, 1997, 390 (6658) :404-407. [6]曾如辉, 罗丽莉, 许铭炎, 等.自体子宫移植建立子宫内膜异位症大鼠模型[J].实验动物与比较医学, 2006, 26 (2) :72-74. [7] [7]LU Z, ZHANG W, JIANG S, ZOU J, Li Y.Effect of lesion location on endometriotic adhesion and angiogenesis in SCID mice[J].Arch Gynecol Obstet, 2014, 289 (4) :823-830. [8]任旭, 何援利, 彭冬先.缺氧诱导因子-1α在子宫内膜异位症的表达与微血管密度的关系[J].广东医学, 2007, 28 (2) :229-231. [9]任旭, 陈茜, 陈小琴.缺氧诱导因子-1α单克隆抗体治疗SCID小鼠子宫内膜异位病灶的实验研究[J].医学临床研究, 2011, 28 (8) :1472-1474. [10] [10]LU Z, ZHANG W, JIANG S, ZOU J, LI Y.Effect of oxygen tensions on the proliferation and angiogenesis of endometriosis heterograft in severe combined immunodeficiency mice[J].Fertil Steril, 2014, 101 (2) :568-576. -
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