摘要:
目的 观察高压氧治疗成年大鼠视神经损伤疗效.方法 使用24只雌性昆明大鼠建立视神经损伤模型, 左眼为视神经损伤眼, 右眼为假手术对照眼, 按照随机数字表法随机分为实验组和对照组, 每组12只.实验组给予高压氧治疗, 每天治疗1 h, 10 d为1个疗程, 共治疗2个疗程, 对照组呼吸常压新鲜空气.分别在治疗过程中的第3、10、20 d检测实验组、对照组大鼠双眼的F-VEP并进行治疗效果评价.结果 对照组中夹持视神经损伤眼比正常眼F-VEP潜伏期延长 (P<0.05) 、波幅降低 (P<0.05) ;实验组正常眼与对照组正常眼F-VEP数据比较潜伏期、波幅均无明显差异 (P>0.05) ;实验组与对照组的视神经损伤眼F-VEP数据比较潜伏期提前 (P<0.05) 、波幅增高 (P<0.05) ;不同时间点测得的F-VEP数据比较具有统计学意义 (P<0.05) .结论 实验大鼠视神经损伤模型建立是成功的;高压氧治疗在视神经未损伤眼中不会引起差异 (P>0.05) , 高压氧治疗是有效的;随着治疗时间的延长损伤视神经恢复越好.
Abstract:
Objective To observe the effects of hyperbaric oxygen in the treatment of the rats with optic nerve crush. Me thods In this study, 24 rats with traumatic optic nerve injury were recruited. The rats' left eyes were injured in the optic nerve and the right eyes as the controls were applied with sham-operation. The rats were divided into the treatment group and the control group in accordance with the random number table. The experimental group was treated with two-course hyperbaric oxygen one hour a day, 10 days a course. The control group breathed fresh air under standard atmospheric pressure. F-VEP was detected on day 3, 10, 20 during the experiment. Re s ults In the control group, latency of F-VEP delayed (P < 0.05) and the amplitude of P100 decreased (P < 0.05) in the injured eye compared to the normal eye. In the experimental group, no obvious difference was found in the latency of F-VEP and the amplitude of P100 in the injured eye compared to the normal eye (P > 0.05) . The comparison of F-VEP data of the injured eyes showed that incubation period was in advance (P < 0.05) and amplitude increased (P < 0.05) in the experimental group. The comparison of F-VEP data tested at different time points was statistical significant. Conclusion The rat model of optic nerve injury was successful. Hyperbaric oxygen therapy showed no interference with the F-VEP data of the injured eye by causing no damage to the normal eye and was proved effective. Longer treatment provided better results
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