脑出血患者血清MicroRNA-124的表达及临床相关因素

保玉莲; 朱榆红; 李勤; 鲍天昊

脑出血患者血清MicroRNA-124的表达及临床相关因素

1. 个旧市人民医院神经内科
2. 昆明医科大学第二附属医院神经内科
3. 昆明医科大学附属精神卫生中心

基金项目:

基金：国家自然科学基金项目 (81060102); 云南省科技计划项目 (2017FE467-152);

计量
- 文章访问数: 1386
- HTML全文浏览量: 495
- PDF下载量: 58
- 被引次数: 0
出版历程
- 收稿日期: 2017-10-19

A Study of microRNA-124 Expression in blood Serum of Patients with Intracerebral Hemorrhage

Funds:

基金：国家自然科学基金项目 (81060102); 云南省科技计划项目 (2017FE467-152);

摘要

摘要: 目的观察microRNA-124在脑出血患者血清中的表达, 并探讨其与脑出血相关临床因素的关系.方法收集同一时期住院的脑出血患者及健康体检者各30份血清标本, 测定microRNA-124在血清中的相对表达量, 比较2组间microRNA-124表达量的变化, 同时进行脑出血患者相关临床资料的对比研究, 探讨microRNA-124与脑出血相关临床因素的关系.结果与正常体检者相比, microRNA-124在脑出血患者血清中的表达升高 (P<0.05) , 其表达量与出血量、发病时间有关 (P<0.05) , 而与性别、出血部位、有无手术治疗 (包括微创、开颅) 、高血压史、空腹静脉全血葡萄糖水平 (FPG) 、既往脑血管病史等因素无明显相关性 (P>0.05) .结论 microRNA-124在脑出血患者血清中的表达量较正常人升高, 且出血量越多, 表达量越高, 在发病1周内较发病1周后表达升高.
- microRNA-124 /
- 表达量 /
- 脑出血
Abstract: Objective To observe microRNA-124 expression in the serum of patient with intracerebral hemorrhage and explore the relationship between the expression and associated clinical factors. Me thods Thirty serum specimen were collected among patients with intracerebral hemorrhage, who were checked up at the same time in the hospital. Relative expression of microRNA-124 in serum was detected. The variation of Micro RNA-124 expression between the two groups and clinical data of patients with intracerebral hemorrhage were analyzed. The relationship between microRNA-124 and clinical factors related to intracerebral hemorrhage was explored. Re s ults Compared with healthy people, microRNA-124 expression in serum increased among patients with intracerebral hemorrhage (P <0.05) . The expression is related to the bleeding volume and onset time (P <0.05) .Meanwhile, no obvious correlation is established between serum microRNA-124 expression and other factors in patients with intracerebral hemorrhage, such as gender, bleeding area, with or without surgical treatment (including minimally invasive and craniotomy) , a history of high blood pressure, fasting venous blood glucose levels (FPG) , and cerebrovascular disease history (P >0.05) . Conclus ion The serum microRNA-124 expression in patients with intracerebral hemorrhage is higher than that in healthy people. Bleeding volume positively increases expression. A higher expression is seen within one week at the onset of intracerebral hemorrhage compared to that one week after.
- MicroRNA-124 /
- Expression /
- Intracerebral hemorrhage

HTML全文

参考文献(14)

[1] 中华医学会神经病学分会脑血管病学组.中国脑出血诊治指南2014[J].中华神经科杂志, 2015, 48 (6) :435-444.
[2]	[2]HARFE B D.Micro RNAs in vertebrate development[J].Curr Opin Genet Dev, 2005, 15 (4) :410-415.
[3]	[3]KAO-PING CHANG, HSIAO-CHEN LEE, SHU-HUNG HUANG, et al.Micro RNA Signatures in Ischemia-Reperfusion Injury[J].Annals of Plastic Surgery, 2012, 69 (6) :668-671.
[4]	[4] SAYED D, ABDELLATIF M.Micro RNAs in development and disease[J].Physiol Rev, 2011, 91 (3) :827-887.
[5]	[5] LUI Z D, TIAN Y, ANDER B P, et al.Brain and blood micro RNA expression profiling of ischemic stroke, intracerebral hemorrhage, and kainite seizures[J].J Cerebral Blood Flow Metab, 2010, 30 (1) :92-101.
[6]彭志锋.下调micro RNA-181b在小鼠缺血性脑损伤中的神经保护作用[J].中国病理生理杂志, 2015, 31 (2) :224-228.
[7]	[7]KENNETH J, THOMAS D.Analysis relative gene expression data using real-time quantitative PCR and the 2-△△Ct Methods[J].Methods, 2011, 25 (4) :402-408.
[8]	[8] CHEN X, BA Y, MA L, et al.Characterization of micro RNAs in serum:A novel class of biomarkers for diagnosis of cancer and other diseases[J].Cell Res, 2008, 18 (10) :997-1006.
[9]	[9] WENG H, SHEN C, HIROKAWA G, et al.Plasma mi R-124 as a biomarker for cerebral infarction[J].Biol Res, 2011, 32 (2) :135-141.
[10]林洋, 芮耀诚.脑缺血大鼠脑组织及血浆中micro RNA-124a表达的变化[J].中国脑血管病杂志, 2011, 8 (3) :143-147.
[11]	[11] DIEHL P, FRICKE A, SANDER L, et al.Microparticles:major transport vehicles for distinct micro RNAs in circulation[J].Cardiovasc Res, 2012, 93 (4) :633-644.
[12]	[12] STEINER T, JUVELA S, UNTERBERG A, et al.European stroke organization guidelines for the management of intracranial aneurysms and subarachnoid haemorrhage[J].Cerebrovasc Dis, 2013, 35 (2) :93-112.
[13]	[13]LATERZA O F, LIM L, GARRETT-ENGELE P W, et al, Plasma micro RNAs as sensitive and specific biomarkers of tissue injury[J].Clin Chem, 2009, 55 (5) :1977-1983.
[14]	[14]LEUNG L Y, CHAN C P, LEUNG Y K, et al.Comparison of mi R-124-3p and mi R-16 for early diagnosis of hemorrhagic ischemic stroke[J].Clin Chim Acta, 2014, 433 (6) :139-144.

相关文章(20)

[1]	王坤, 蒲军, 杨涛, 王路, 李东波. 不同入路显微镜手术治疗青年重症基底节区高血压脑出血的疗效比较, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20240708
[2]	汪鑫邦, 胡安, 赵喜鹏, 汪炎, 丁伟, 操礼鹏, 古禹, 许轲, 张云峰. Ommaya囊治疗脑出血并发急性期脑积水疗效及对血脑屏障功能的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20241015
[3]	赵广平, 刘盼盼, 刘飞, 李炜, 陈永学, 张凯. 高血压脑出血立体定向颅内血肿清除术中不同麻醉方式的血流动力学及应激反应, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220521
[4]	王应霞, 何琴, 王国芳, 张旋. 非小细胞肺癌中microRNA-21和EGFR的表达及意义, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210818
[5]	阎婷婷, 田丹, 李新, 俎云芬, 李崇阳, 尚学琴. MicroRNA Let-7a与p53基因在肺癌中表达的相关性, 昆明医科大学学报.
[6]	李明. 高血压基底节区脑出血实施经外侧裂入路的手术治疗, 昆明医科大学学报.
[7]	湛自兰. 护理干预对脑出血非手术患者心理状态、并发症以及满意度的影响, 昆明医科大学学报.
[8]	魏亮, 王琦, 杨成, 张燕飞, 管宏新, 孙志扬. 亚低温治疗对脑出血所致MODS的影响, 昆明医科大学学报.
[9]	王丹. 血清低密度脂蛋白胆固醇水平与脑出血的相关性初探, 昆明医科大学学报.
[10]	王海峰. 上调microRNA-101沉默EZH2基因表达对人膀胱癌T24细胞系增殖和凋亡的影响, 昆明医科大学学报.
[11]	刘乐万. 钻孔穿刺引流术治疗高血压脑出血的临床应用研究, 昆明医科大学学报.
[12]	倪忠梅. 高血压脑出血患者焦虑、抑郁状态的护理干预, 昆明医科大学学报.
[13]	黄微. 脑出血与脑梗死并发腹泻发生率的临床研究, 昆明医科大学学报.
[14]	高莉萍. 高血压性脑出血微创手术与保守治疗的临床疗效对比分析, 昆明医科大学学报.
[15]	张蕾. 微创穿刺治疗高血压性脑出血112例临床分析, 昆明医科大学学报.
[16]	董建平. 神经外科患者气管切开治疗53例临床分析, 昆明医科大学学报.
[17]	刘泽飞. 额部钻孔置管引流术治疗高血压性脑出血, 昆明医科大学学报.
[18]	郭昌贵. 依达拉奉治疗急性脑出血的临床疗效观察, 昆明医科大学学报.
[19]	. 高血压脑出血合并糖尿病的术式选择及围手术期处理, 昆明医科大学学报.
[20]	庄永辉. 微创尿激酶引流治疗高血压脑出血, 昆明医科大学学报.