滇重楼巴布剂对大鼠急性痛风性关节炎及炎症的调控机制
The Research of Effects of Applying Cataplasm of Paris Polyphylla Smith Var. Yunnanensis (Franch.) Hand.-Mazz on Acute Gouty Arthritis and Inflammation
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摘要: 目的 拟改进云南省中医学院第三附属医院骨科传统外敷药剂型, 筛选其中重要有效成分滇重楼制成巴布剂, 并对其炎症调控机制进行研究.方法 SD雄性大鼠随机分为对照组和治疗组2组, 于大鼠足内踝胫跗关节腔局部注射5%尿酸钠混悬液50μL, 制成急性痛风性关节炎模型.对照组外敷无任何药物的巴布剂, 治疗组外敷滇重楼巴布剂, 分别检测4、24、48、72 h后2组大鼠踝关节直径、周长指标的变化.ELISA法检测外周血白介素-1β (IL-1β) 和肿瘤坏死因子-α (TNF-α) 的变化, Western blotting法检测单核细胞TLR4的表达水平.结果 与对照组相比, 滇重楼巴布剂外敷治疗组, 足踝直径及周径在各时间点增加值明显减少, IL-1β在4、24、72 h下降, TLR4表达在48 h降低.结论 重楼巴布剂可通过降低炎症因子IL-1β与TLR4的表达, 减轻大鼠急性痛风性关节炎症反应.Abstract: Objective This study is designed to improve topical medication in the Department of Orthopedics in Kunming Municipal Hospital of Traditional Chinese Medicine by applying cataplasm made from the active ingredient (polyphyllin) of Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz ( "Dian Chonglou" in Chinese) and to study the mechanism of anti-inflammation. Me thods SD male rats were randomly divided into two groups: control group and treatment group. The rats in two groups were all injected 50 ul 5% sodium urate suspension into tibiotarsal joint cavity to duplicate the model of acute gouty arthritis.The rats in the control group were applied with cataplasm without polyphyllin, whereas those in the treatment group applied with cataplasm of polyphyllin. After 4, 24, 48, 72 hours, the ankle diameter and circumference were measured. ELISA was used to test the interleukin-1β (IL-1β) andtumor necrosis factor (TNF) in peripheral blood. Western blotting was applied to detect the changes in TLR4 expression of monocyte.Re s ults Compared with the control group, the added value of the diameter and circumference of ankle decreased significantly in the treatment group 4, 24, 48 and 72 h after the treatment.IL-1β in the treatment group was lowered at 4, 24 and 72 h. TLR4 in the treatment group decreased at 48 h. Conclus ion Cataplasm with polyphyllin can alleviate the acute inflammatory reaction in gouty joint of rat by reducing the expression of IL-1βand TLR4.
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