恒古骨伤愈合剂通过TGFβ/Smad信号通路促进去势骨质疏松症大鼠BMSC增殖

郑红; 李进涛; 袁鑫; 吴超; 曾河; 赵宏斌; 唐薇

恒古骨伤愈合剂通过TGFβ/Smad信号通路促进去势骨质疏松症大鼠BMSC增殖

郑红¹,
李进涛¹,
袁鑫²,
吴超¹,
曾河¹,
赵宏斌²,
唐薇²

1. 昆明医科大学实验动物学部
2. 昆明医科大学第一附属医院急诊医学部

基金项目:

基金：国家自然科学基金资助项目 (81460647); 云南省应用基础研究计划项目 (2014FB033;2017FE-014&-016);

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出版历程
- 收稿日期: 2017-10-21

Osteoking Promotes the Proliferation of BMSC through Activating TGF-β/Smad Signaling Pathway in Castrate Osteoporosis Rats

Funds:

基金：国家自然科学基金资助项目 (81460647); 云南省应用基础研究计划项目 (2014FB033;2017FE-014&-016);

摘要

摘要: 目的研究恒古骨伤愈合剂 (OK) 通过TGFβ/Smad信号通路对骨质疏松 (OP) 大鼠骨髓基质干细胞 (BMSCs) 细胞分化的作用.方法摘除大鼠双侧卵巢, 复制OP模型, 分离、培养BMSCs.将源于假手术大鼠的BMSCs常规培养作为正常对照组, 将源于OP大鼠的BMSCs分为5组, OP模型组常规培养, 阳性对照组加入1μmol/L阿仑膦酸钠, OK低、中、高治疗组分别加入恒古骨伤愈合剂50 mL/L、100 mL/L和200 mL/L, 孵育24 h, 收集各组细胞.采用MTT法检测BMSCs增殖率, ELISA法检测细胞骨形成标志物含量, RT-qPCR法检测TGF-β的m RNA表达水平, Western blot法检测TGF-β、p-Smad2/3以及Smad2/3的蛋白表达水平.结果与正常对照组比较, OP模型对照组BMSCs细胞增殖率降低, 骨形成标志物OC、PINP和BALP含量降低, TGF-β的m RNA和蛋白表达水平降低, Smad2/3的磷酸化水平降低, 差异有统计学意义 (P<0.05) .经OK治疗后, 与模型组比较, 上述均有不同程度缓解, OK组疗效呈现出剂量依赖性, 且1μmol/L阿仑膦酸钠、100mL/L和200 mL/L OK组治疗效果均有统计学意义 (P<0.05) .结论 OK可以通过激活TGF-β/Smad信号通路促进成骨细胞增殖, 从而达到治疗绝经后OP的效果.
- 恒古骨伤愈合剂 /
- 骨质疏松 /
- 骨髓基质干细胞 /
- TGF-β/Smad信号通路
Abstract: Objective To study the effect of OK on the differentiation of bone marrow stromal stem cells (BMSCs) through the TGFβ/Smad signaling pathway in rats. Methods We removed the bilateral ovaries of rats to replicate OP model, and isolated and cultured BMSCs. BMSCs isolated from nomal rats were cultured as control group, BMSCs isolated from OP rats were divided into 5 groups, OP model group was regularly cultured, positive control group was treated with alendronate sodium (1 μmol/L) , low, medium and high OK treatment group were treated with 50 mL/L, 100 mL/L and 200 ml/L OK respectively.After 24 h incubation, all cells were collected. The proliferation rate of BMSCs was determined by MTT method, and ELISA method was employed to detect the contents of bone formation markers, RT-qPCR was used to determine the m RNA expression level of TGFβ and the protein expression levels of TGFβ, p-Smad2/3 and of Smad2/3 were detected by Western blot. Results Compared with control group, the proliferation rate of the BMSCs in OP model group was reduced, concentrations of bone formation markers (OC, PINP and BALP) were reduced, m RNA and protein expression levels of TGFβ, as well as the phosphorylated level of Smad2/3 were downregulated.The difference was statistically significant (P<0.05) . After treatment with OK, compared with model group, all the above effects were ameliorated in different degree, a dose dependent manner was observed in OK treatment group, and the treatment effects of alendronate sodium (1 μmol/L) , 100 mL/L and 200 mL/L OK group were statistically significant (P <0.05) .Conclusion OK can promote the proliferation of osteoblasts by activating TGFβ/Smad signaling pathway to achieve the effect of treating postmenopausal OP.
- Osteoking /
- Osteoporosis /
- Bone marrow stromal stem cells /
- TGFβ/Smad signaling pathway

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