新型抗菌物质DAPC对变异链球菌粘附作用的影响
Effect of DAPC on Adhesion of Streptococcus Mutans
-
摘要: 目的 研究新型抗菌物质新型氨基酸衍生物抗菌物质-焦谷氨酸双癸基二甲基氨基羧酸盐 (N, N-di-n-decyl-N, N-dimethyl-ammonium 5-oxopyrrolidine-2-carboxylate, DAPC) 对变异链球菌粘附的影响.方法采用2倍稀释法测定DAPC和葡萄糖酸氯己定对变异链球菌的最低抑菌浓度和最低杀菌浓度.在此基础上, 以葡萄糖酸氯己定为阳性对照, PBS为阴性对照, 结晶紫法测定比较短时浸泡药物后釉质片表面生物膜的形成量.结果DAPC对变异链球菌的MIC为0.003 125 0%, MBC为0.006 250 0%;葡萄糖酸氯己定对变异链球菌的MIC为0.001 562 5%, MBC为0.003 125 0%.2种药物MIC浸泡后生物膜形成量与PBS浸泡组差异无统计学意义 (P>0.05) .而随着浓度升高, 当葡萄糖酸氯己定和DAPC浓度分别为0.12%和0.24%时, 实验组生物膜形成量显著低于PBS组 (P<0.01) , 且在24 h后与葡萄糖酸氯己定组差异无统计学意义 (P>0.05) .结论 DAPC对变异链球菌有明显的抗菌活性, 可持续较长时间抑制细菌粘附作用.Abstract: Objective To analyze the property of the new antibacterial agent (N, N-di-n-decyl-N, N-dimethyl-ammonium 5-oxopyrrolidine-2-carboxylate, DAPC) to prevent Streptococcus mutans' adhesion on tooth surface.Method determine the minimal inhibitory concentration and minimal bactericidal concentration of DAPC and Chlorhexidine gluconate by liquid dilution method.Set the Chlorhexidine gluconate as the positive control, while PBS as the negative control.Use the crystal violet staining to measure the quantity of biofilm.Re s ult The MIC of DAPC on Streptococcus mutans was 0.0031250% and MBC was 0.0062500% . The MIC of Chlorhexidine gluconate was 0.0015625% , while the MBC was 0.0031250% .When concentration of the two antibacterial agents was MIC, the quantity of biofilm have no significance among three groups.However, when concentration of Chlorhexidine gluconate and DAPC were 0.12% and 0.24% respectively, biofilm of experimental group was lower than PBS, and there is no significance between Chlorhexidine gluconate group and DAPC group after 24 hours incubation.Conclus ion New antibacterial agent DAPC have significant effect in inhibiting of Streptococcus mutans.And the residual of DAPC on teeth can maintain a long time of antibacterial effect to inhibit Streptococcus mutans' adhesion.
-
Key words:
- DAPC /
- Chlorhexidine gluconate /
- Streptococcus mutans /
- Adhesion
-
[1]周学东.实用口腔微生物学与技术[M].北京:人民卫生出版社, 2009:191. [2] [2]RK C.Oxygenating agents may inhibit tooth staining effect of chlorhexidine[J].The Journal of Evidence-Based Dental Practice, 2013, 13 (1) :9-10. [3]陆可望, 陆可信, 张磊.一种氨基酸双链季氨基羧酸盐、制备方法及用作杀微生物剂[P].美国:CN103012237A, 2013-04-03. [4] [4]AUSCHILL T M, HEIN N, HELLWIG E, et al.Effect of two antimicrobial agents on early in situ biofilm formation[J].Journal of Clinical Periodontology, 2005, 32 (2) :147-152. [5] [5]WIMPENNY J, MANZ W, SZEWZYK U.Heterogeneity in biofilms[J].Fems Microbiology Reviews, 2000, 24 (5) :661. [6]樊明文.口腔生物学[M].北京:人民卫生出版社, 2004:190-194. [7] [7]LANG N, BRECX M C.Chlorhexidine digluconate-an agent for chemical plaque control and prevention of gingival inflammation[J].Journal of Periodontal Research, 2010, 21 (s16) :74-89. [8] [8]ITO T, ICHINOSAWA T, SHIMIZU T.Streptococcal adhesin Ssp A/B analogue peptide inhibits adherence and impacts biofilm formation of Streptococcus mutans[J].Plos One, 2017, 12 (4) :e0175483. [9]张晨, 刘雅丽, 陈思佳, 等.柠檬精油、柠檬烯、茶多酚对变形链球菌表面疏水性及黏附的影响[J].实用口腔医学杂志, 2016, 32 (4) :471-474. [10] [10]LATIMER J, MUNDAY J L, BUZZA K M, et al.Antibacterial and anti-biofilm activity of mouthrinses containing cetylpyridinium chloride and sodium fluoride[J].Bmc Microbiology, 2015, 15 (1) :169. [11] [11]BONESVOLL P, LKKEN P, RLLA G, et al.Retention of chlorhexidine in the human oral cavity after mouth rinses[J].Archives of Oral Biology, 1974, 19 (11) :1025-1029. [12] [12]MURATA H, KOEPSEL R R, MATYJASZEWSKI K, et al.Permanent, non-leaching antibacterial surface-2:how high density cationic surfaces kill bacterial cells[J].Biomaterials, 2007, 28 (32) :4870-4879. -
点击查看大图
计量
- 文章访问数: 1922
- HTML全文浏览量: 669
- PDF下载量: 74
- 被引次数: 0
下载:
