灯盏花乙素对人心脏微血管内皮细胞中PKCε表达的调控作用

陈晨; 王友兰; 刘伟军; 饶嫱; 杜庭彦; 杜晓华; 杨为民; 朱秀芬

灯盏花乙素对人心脏微血管内皮细胞中PKCε表达的调控作用

1. 昆明医科大学药学院暨云南省天然药物药理重点实验室
2. 昆明市医学科学研究所
3. 曲靖市第一人民医院药剂科

基金项目:

基金：国家自然科学基金资助项目 (81560589, 30960450, 81173110); 云南省科技厅联合专项基金资助项目[ (2014FA010, 2017FE467 (-019) ];

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出版历程
- 收稿日期: 2018-01-12

Regulation of Scutellarin on PKCε in Human Cardiac Microvascular Endothelial Cells

Funds:

基金：国家自然科学基金资助项目 (81560589, 30960450, 81173110); 云南省科技厅联合专项基金资助项目[ (2014FA010, 2017FE467 (-019) ];

摘要

摘要: 目的探讨灯盏花乙素 (scutellarin, SCU) 对正常及缺氧再给氧 (hypoxia reoxygenation, HR) 处理时人心脏微血管内皮细胞 (human cardiac microvascular endothelial cells, HCMECs) 中PKCε蛋白及m RNA表达的调控作用.方法以正常与缺氧再给氧2种方式培养HCMECs, 实验分组为:在正常培养实验中分为正常对照 (Control) 组、SCU (0.1μM, 1μM, 10μM) 3种剂量组;HR培养实验中分为Control组、Model (HR) 组、HR处理的SCU (0.1μM, 1μM, 10μM) 3种剂量组.分别以Western blot与RT-PCR方法检测HCMECs中p-PKCε与PKCε蛋白与m RNA表达的变化.结果在正常培养的HCMECs中, SCU (0.1μM, 1μM, 10μM) 剂量组均可显著上调PKCε的蛋白及m RNA的表达 (P<0.05) , 对p-PKCε也有上调趋势;在HR培养的细胞实验中, Model组PKCε有下调趋势, 而SCU 10μM组可显著上调PKCε的蛋白及m RNA的表达 (P<0.05) , 并且SCU 10μM亦可显著上调p-PKCε (P<0.05) .结论 SCU对正常及HR处理时HCMECs中PKCε蛋白及m RNA的表达有明显的上调作用.
- 灯盏花乙素 /
- 人心脏微血管内皮细胞 /
- 缺氧再给氧 /
- PKCε
Abstract: Objective To investigate the regulation of scutellarin (SCU) on protein and m RNA expression of PKCε in Human cardiac microvascular endothelial cells (HCMECs) induced by hypoxia reoxygenation or normal. Me thods We cultured HCMECs in two ways: normal and hypoxia reoxygenation (HR) . The normal groups were divided into one control group and three SCU (0.1 μM, 1 μM, 10 μM) groups;HR treatment induded one control group and four groups of HR, HR+ SCU 0.1 μM, HR+SCU 1 μM and HR+SCU 10 μM. Protein and m RNA expression of PKCε was determined by Western blot and RT-PCR respectively.Re s ults In normal cultured, SCU (0.1 μM, 1 μM and 10 μM) significantly up-regulated the protein and m RNA expression of PKCε (P <0.05) , and for p-PKCε, there was an up-regulated tendency. In the HR treatment, the model group had a down regulation tendency, but SCU 10 μM group could significantly up-regulate the protein and m RNA expression of PKCε (P <0.05) , and SCU 10 μM also could significantly up-regulated p-PKCε (P <0.05) . Conclus ion SCU up-regulates the expression of PKCε protein and m RNA in HCMECs induced by hypoxia reoxygenation or normal.
- Scutellarin /
- HCMECs /
- hypoxia reoxygenation /
- PKCε

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参考文献(19)

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