灯盏花乙素对人心脏微血管内皮细胞中PKCε表达的调控作用

陈晨; 王友兰; 刘伟军; 饶嫱; 杜庭彦; 杜晓华; 杨为民; 朱秀芬

灯盏花乙素对人心脏微血管内皮细胞中PKCε表达的调控作用

1. 昆明医科大学药学院暨云南省天然药物药理重点实验室
2. 昆明市医学科学研究所
3. 曲靖市第一人民医院药剂科

基金项目:

基金：国家自然科学基金资助项目 (81560589, 30960450, 81173110); 云南省科技厅联合专项基金资助项目[ (2014FA010, 2017FE467 (-019) ];

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出版历程
- 收稿日期: 2018-01-12

Regulation of Scutellarin on PKCε in Human Cardiac Microvascular Endothelial Cells

Funds:

基金：国家自然科学基金资助项目 (81560589, 30960450, 81173110); 云南省科技厅联合专项基金资助项目[ (2014FA010, 2017FE467 (-019) ];

摘要

摘要: 目的探讨灯盏花乙素 (scutellarin, SCU) 对正常及缺氧再给氧 (hypoxia reoxygenation, HR) 处理时人心脏微血管内皮细胞 (human cardiac microvascular endothelial cells, HCMECs) 中PKCε蛋白及m RNA表达的调控作用.方法以正常与缺氧再给氧2种方式培养HCMECs, 实验分组为:在正常培养实验中分为正常对照 (Control) 组、SCU (0.1μM, 1μM, 10μM) 3种剂量组;HR培养实验中分为Control组、Model (HR) 组、HR处理的SCU (0.1μM, 1μM, 10μM) 3种剂量组.分别以Western blot与RT-PCR方法检测HCMECs中p-PKCε与PKCε蛋白与m RNA表达的变化.结果在正常培养的HCMECs中, SCU (0.1μM, 1μM, 10μM) 剂量组均可显著上调PKCε的蛋白及m RNA的表达 (P<0.05) , 对p-PKCε也有上调趋势;在HR培养的细胞实验中, Model组PKCε有下调趋势, 而SCU 10μM组可显著上调PKCε的蛋白及m RNA的表达 (P<0.05) , 并且SCU 10μM亦可显著上调p-PKCε (P<0.05) .结论 SCU对正常及HR处理时HCMECs中PKCε蛋白及m RNA的表达有明显的上调作用.
- 灯盏花乙素 /
- 人心脏微血管内皮细胞 /
- 缺氧再给氧 /
- PKCε
Abstract: Objective To investigate the regulation of scutellarin (SCU) on protein and m RNA expression of PKCε in Human cardiac microvascular endothelial cells (HCMECs) induced by hypoxia reoxygenation or normal. Me thods We cultured HCMECs in two ways: normal and hypoxia reoxygenation (HR) . The normal groups were divided into one control group and three SCU (0.1 μM, 1 μM, 10 μM) groups;HR treatment induded one control group and four groups of HR, HR+ SCU 0.1 μM, HR+SCU 1 μM and HR+SCU 10 μM. Protein and m RNA expression of PKCε was determined by Western blot and RT-PCR respectively.Re s ults In normal cultured, SCU (0.1 μM, 1 μM and 10 μM) significantly up-regulated the protein and m RNA expression of PKCε (P <0.05) , and for p-PKCε, there was an up-regulated tendency. In the HR treatment, the model group had a down regulation tendency, but SCU 10 μM group could significantly up-regulate the protein and m RNA expression of PKCε (P <0.05) , and SCU 10 μM also could significantly up-regulated p-PKCε (P <0.05) . Conclus ion SCU up-regulates the expression of PKCε protein and m RNA in HCMECs induced by hypoxia reoxygenation or normal.
- Scutellarin /
- HCMECs /
- hypoxia reoxygenation /
- PKCε

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参考文献(19)

[1]	[1]WANG M, SUN G B, ZHANG J Y, et al.Elatoside C protects the heart from ischaemia/reperfusion injury through the modulation of oxidative stress and intracellular Ca2+homeostasis[J].Int J Cardiol, 2015, 185 (2) :167-176.
[2]	[2]GROVER-PAEZ F, ZAVALZA-GMEZ AB.Endothelial dysfunction and cardiovascular risk factors[J].Diabetes Res Clin Pract, 2009, 84 (1) :1-10.
[3]	[3]MARX N, GRANT P J.Endothelial dysfunction and cardiovascular disease the lull before the storm[J].Diab Vasc Dis Res, 2007, 4 (2) :82-83.
[4]	[4]STEINBERG S F.Structural basis of protein kinase C isoform function[J].Physiol Rev, 2008, 88 (4) :1341-1378.
[5]	[5]LAI L, ZHANG X, ZHANG X, et al.Lazaroid U83836E protects the heart against ischemia reperfusion injury via inhibition of oxidative stress and activation of PKC[J].Mol Med Rep 2016, 13 (1) :3993-4000.
[6]	[6]AKITA Y.Protein kinase C-epsilon (PKC-epsilon) :Iits unique structure and function[J].J Biochem, 2002, 132 (6) :847-852.
[7]	[7]CHEN Y, TIAN Q.The role of protein kinase C epsilon in neural signal transduction and neurogenic diseases[J].Front Med, 2011, 5 (1) :70-76.
[8]	[8]HUND T J, LERNER D L, YAMADA K A, et al.Protein kinase Cepsilon mediates salutary effects on electrical coupling induced by ischemic preconditioning[J].Heart Rhythm, 2007, 4 (9) :1183-1193.
[9]	[9]INAGAKI K, CHURCHILL E, MOCHLY-ROSEN D.Epsilon protein kinase C as a potential therapeutic target for the ischemic heart[J].Cardiovas Res, 2006, 70 (15) :222-230.
[10]	[10]HAO Z, PAN S S, SHEN Y J, et al.Exercise preconditioning-induced early and late phase of cardioprotection is associated withprotein kinase C epsilon translocation[J].Circ J, 2014, 78 (7) :1636-1645.
[11]	[11]TANG L, PENG Y, XU T, et al.The effects of quercetin protect cardiomyocytes from A/R injury is related to its capability to increasing expression and activity of PKC epsilon protein[J].Mol Cell Biochem, 2013, 382 (1) :145-152.
[12]	[12]LIN L L, LIU A J, LIU J G, et al.Protective effects of scutellarin and breviscapine on brain and heart ischemia in rats[J].J Cardiovasc Pharmacol, 2007, 50 (1) :327-332.
[13]	[13]ZHANG H F, HU X M, WANG L X, et al.Protective effects of scutellarin against cerebral ischemia in rats:evidence for inhibition of the apoptosis-inducing factor pathway[J].Planta Med, 2009, 75 (1) :121-126.
[14]	[14]XIAOHUA D U, CHEN CHEN, MIN ZHANG, et al.Scutellarin reduces endothelium dysfunction through the PKG-I pathway[J].Evidence-Based Complementary and Alternative Medicine, 2015, 2015 (1) :1-12.
[15]张人伟, 张元玲, 王杰生.灯盏花黄酮类成分的分离鉴定[J].中草药, 1988, 19 (5) :7-9.
[16]朱君荣, 李运曼.灯盏花素冰片注射液对实验性脑缺血的保护作用[J].中国药科大学学报, 2007, 38 (5) :456-460.
[17]	[17]YANG W, LUST R M, BOFFERDING A, et al.Nitric oxide and catalase-sensitive relaxation by scutellarin in the mouse thoracic aorta[J].J Cardiovasc Pharmacol, 2009, 53 (1) :66-76.
[18]杨翼, 李章华, 石武谛, 等.热预处理对超负荷训练大鼠心肌蛋白激酶C表达的影响[J].中国康复理论与实践, 2015, 21 (1) :49-53.
[19]	[19]KORZICK D H, RISHEL ME, BOWLES D K.Exercise and hypercholesterolemia produce disparate shifts in coronary PKC expression[J].Med Sci Sports Exerc, 2005, 37 (3) :381-388.

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