特利加压素联合FK-409在大鼠减体积肝移植模型中对移植肝的保护作用

莽源祎; 李立; 冉江华; 张升宁; 刘静; 刘剑; 高杨; 李来邦

特利加压素联合FK-409在大鼠减体积肝移植模型中对移植肝的保护作用

昆明医科大学附属甘美医院昆明市第一人民医院肝胆一血管外科

基金项目:

基金：云南省科技厅昆明医科大学应用基础研究联合专项基金资助项目 (2010CD213);

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出版历程
- 收稿日期: 2017-12-19

The Effect of Terlipressin and FK-409 on Protection of Liver Graft Injury after Reduced-Size Liver Transplantation in Rats

Funds:

基金：云南省科技厅昆明医科大学应用基础研究联合专项基金资助项目 (2010CD213);

摘要

摘要: 目的在大鼠减体积肝移植模型中联合应用特利加压素和FK-409探究其对受体移植肝的保护作用.方法使用30%体积供肝行SD大鼠同系肝移植, 监测术后门静脉压力、血谷丙转氨酶 (ALT) 、总胆红素水平和术后生存时间, 构建有效的减体积肝移植模型.利用减体积肝移植模型作为载体, 根据不同的干预手段将受体大鼠分为特利加压素处理组、FK-409处理组、特利加压素处理联合FK-409处理组和对照组, 监测术后各组门静脉压力、血谷丙转氨酶 (ALT) 、总胆红素水平和术后生存时间, 统计并分析特利加压素处理联合FK-409处理对减体积肝移植移植肝的保护作用.结果在减体积肝移植模型中, 特利加压素联合FK-409处理组与对照组比较, 术后门静脉压力显著小于对照组, 差异有统计学意义 (P<0.05) , 术后血清ALT、总胆红素显著低于对照组, 差异有统计学意义 (P<0.05) , 术后生存时间较对照组明显延长, 差异有统计学意义 (P<0.05) , 在减体积肝移植模型中联合使用特利加压素、FK-409可有效保护移植肝脏.结论特利加压素联合FK-409可有效保护大鼠减体积肝移植中移植肝, 特利加压素联合FK-409方案可能成为减体积肝移植术后小肝综合症防治的潜在药物处理方案.
- 特利加压素 /
- FK-409 /
- 减体积肝移植
Abstract: Object To detect the effect of terlipressin and FK-409 on protection of liver graft injury after reduced-size liver transplantation in rats. Me thods We performed liver transplantation using 30% volume liver graft in rats. ALT, total bilirubin, portal vein pressure and survival rate were detected after operation. We divided recipient rats into four groups including terlipressin treatment group, FK-409 treatment group, terlipressin groups and FK-409 treatment group. ALT, total bilirubin, portal vein pressure and survival rate were detected in each group after operation. We compared results of four groups and found potential therapy which protected liver graft injury after reduced-size liver transplantation. Re s ults In reduced-size liver transplantation model, terlipressin and FK-409 treatment group showed lower portal vein pressure (P <0.05) , lower level of ALT and bilirubin (P<0.05) and longer survival time (P<0.05) compared with the control group. Using terlipressin and FK-409 could reduce liver graft injury after reduced-size liver transplantation. Conclus ion Using terlipressin and FK-409 could reduced liver graft injury, which might be the potential therapy for small-for-size syndrome after reduced-size liver transplantation.
- Terlipressin /
- FK-409 /
- Reduced-size liver transplantation

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参考文献(21)

[1]	[1]MEIRELLES J, NIOR R F, SALVALAGGIO P, et al.Liver transplantation:history, outcomes and perspectives[J].Einstein, 2015, 13 (1) :149.
[2]	[2]YAGI S, NAGAI K, KADABA P, et al.Impact of oxygen free radicals on liver graft microcirculation and function after partial liver transplantation in rats[J].Liver Transplantation, 2010, 45 (10) :S126-S126.
[3]	[3]RAJAKUMAR A, KALIAMOORTHY I, RELA M, et al.Small-for-size syndrome:bridging the gap between liver transplantation and graft recovery[J].Seminars in Cardiothoracic&Vascular Anesthesia, 2017, 21 (3) :252.
[4]	[4]DAHM F, GEORGIEV P, CLAVIEN P A.Small-for-size syndrome after partial liver transplantation:definition, mechanisms of disease and clinical implications[J].American Journal of Transplantation, 2005, 5 (11) :2605.
[5]	[5]SALMAN A, ELGAREM N, SHOLKAMY A, et al.Exploring portal vein hemodynamic velocities as a promising, attractive horizon for small-for-size syndrome prediction after living-donor liver transplantation:an egyptian center study.[J].Transplant Proc, 2016, 48 (6) :2135-2139.
[6]	[6]ITO T, KIUCHI T, YAMAMOTO H, et al.Changes in portal venous pressure in the early phase after living donor liver transplantation:pathogenesis and clinical implications[J].Transplantation, 2003, 75 (8) :1313-1317.
[7]	[7]TUCKER O N, HEATON N.The'small for size'liver syndrome.[J].Current Opinion in Critical Care, 2005, 11 (2) :150.
[8]李来邦, 陈奕明, 张升宁, 等.小体积肝移植动物模型的建立[J].昆明医科大学学报, 2014, 35 (11) :31-34.
[9] 冯彦虎, 谷保红, 胡继科, 等."灌溉法"防治小体积肝移植后门静脉高压[J].中华肝胆外科杂志, 2017, 23 (5) :237-331.
[10] 中华医学会器官移植学分会.中国活体肝移植小肝综合征临床诊治指南 (2016版) [J].中华移植杂志:电子版, 2017, 11 (10) :70-74.
[11]郝保兵, 张峰.活体肝移植小肝综合症的防治[J].肝胆外科杂志, 2015, 23 (4) :243-244.
[12]朱志军.小肝综合征的防治策略与进展[J].中国普外基础与临床杂志, 2017, 23 (8) :923-926.
[13]	[13]MIANO C, GARCIA-TSAO G.Clinical pharmacology of portal hypertension[J].Gastroenterology Clinics of North America, 2010, 39 (3) :681-695.
[14]	[14]SAND BOWN L, RICKSTEN S, HOULTZ E, et al.Vasopressin induced changes in splanchnic blood flow and hepatic and portal venous pressures in liver resection[J].Acta Anaesthesiologica Scandinavica, 2016, 60 (5) :607-615.
[15]	[15]HUSSAIN Q, HAIDER S, SOLANGI N M, et al.Estimating effect of terlipressin on portal pressure in cirrhosis by observing hepatic vein Doppler waveform[J].Jpma the Journal of the Pakistan Medical Association, 2013, 63 (5) :604-608.
[16]夏正新, 张志飞.特利加压素联合生长抑素治疗肝硬化失代偿期并急性上消化道大出血的疗效观察[J].药物评价研究, 2017, 39 (12) :1745-1748.
[17]	[17]REN Z, XU Y, ZHU S.The effect of terlipressin on hepatic hemodynamics in small for size livers[J].Hepato gastroenterology, 2012, 59 (113) :208.
[18]蓝柳根, 彭民浩, 卢景宁, 等.FK409对大鼠肝脏移植缺血再灌注损伤后JAK2/STAT3信号通路的影响[J].肝胆外科杂志, 2008, 16 (5) :386-389.
[19]熊赓, 李立.血小板活化因子对肝移植缺血再灌注损伤的影响[J].吉林医学, 2013, 34 (5) :922-23.
[20]尚维伟, 熊奇如.一氧化氮减轻肝移植缺血再灌注损伤的研究进展[J].肝胆外科杂志, 2012, 20 (3) :233-235.
[21]	[21]MAN K, LEE T K, LIANG T B, et al.FK 409 ameliorates small for size liver graft injury by attenuation of portal hypertension and down regulation of Egr-1 pathway.[J].Annals of Surgery, 2004, 240 (1) :159.

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