干扰CD151基因表达对裸鼠肺腺癌A549细胞肺转移的实验
Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549
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摘要: 目的 探讨RNA干扰抑制CD151基因表达对肺腺癌A549细胞肺转移的影响.方法 将构建的RNA干扰抑制CD151基因表达的稳转细胞A549-sh RNA、A549-sh NC、A549-Blank 3组细胞通过尾静脉注射入裸鼠BALB/c-nu, 30 d后解剖, 通过肉眼和HE染色后观察肺转移情况.结果 A549-sh RNA、A549-sh NC、A549-Blank各组裸鼠肺转移率及肺组织上的转移病灶数依次为:62.5% (5/8) 与 (4.7±1.5) , 100% (7/7) 与 (9.3±0.8) , 75% (6/8) 与 (8.2±1.5) .转移病灶数, A549-sh RNA与A549-sh NC (P=0.000) , A549-sh RNA与A549-Blank (P=0.006) 比较, 差异有统计学意义.结论 RNA干扰抑制CD151基因表达能减少A549细胞裸鼠的肺转移.CD151可能成为抑制NSCLC侵袭和转移的潜在的治疗靶点之一.Abstract: Objective To explore the influence of CD151 inhibition by RNA interference on lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. METHODS: Constructed CD151 inhibition by RNA interference cell lines A549-sh RNA, A549-sh NC and A549-Blank were injected into the tail veins of BALB/c nude mice. Lung metastasis was measured through naked eye and pathology of HE stain. Re s ults Lung metastasis were 62.5% (5/8) and (4.7±1.5) , 100% (7/7) and (9.3±0.8) , 75% (6/8) and (8.2±1.5) , respectively in A549-sh RNA, A549-sh NC and A549-Blank. There were differences between A549-sh RNA and A549-sh NC (P = 0.000) and between A549-sh RNA and A549-Blank (P = 0.006) .Conclus ions Inhibition of CD151 gene expression can reduce lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. CD151 may become one of the potential therapeutic targets aiming at inhibitung invasion and metastasis of NSCLC.
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Key words:
- CD151 /
- Non-small cell lung cancer /
- BALB/c-nude mice /
- Lung metastasis
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