人参皂苷Rg1对NAFLD动物模型中同型半胱氨酸的调控机制

侯云鹤; 张皓鑫; 王依雨; 苏艳梅; 顾丹珊; 林少芳; 杨世昆; 李树德

人参皂苷Rg1对NAFLD动物模型中同型半胱氨酸的调控机制

1. 昆明医科大学基础医学院生物化学与分子生物学系
2. 昆明医科大学
3. 昆明医科大学基础医学院细胞生物学与医学遗传学系
4. 昆明市第一人民医院干疗科
5. 昆明医科大学第一附属医院移植科

基金项目:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2015FB046, 2017FE467); 云南省教育厅科学研究基金资助项目 (2017YJS076); 云南省大学生创新创业训练计划基金资助项目 (2017); 昆明医科大学大学生创新性实验计划基金资助项目 (2017) ;昆明医科大学硕士研究生创新基金资助项目 (2017S054);

计量
- 文章访问数: 2461
- HTML全文浏览量: 914
- PDF下载量: 108
- 被引次数: 0
出版历程
- 收稿日期: 2017-08-21

Regulatory Mechanism of Ginsenoside Rg1 on Homocysteine in NAFLD Model

Funds:

摘要

摘要: 目的建立非酒精性脂肪性肝病 (NAFLD) 的动物模型, 探讨人参皂苷Rg1调控同型半胱氨酸的分子机制.方法建立NAFLD动物模型, 测定正常对照组和模型对照组空腹状态下的血清甘油三酯 (TG) 和总胆固醇 (TC) , HE染色检测肝脏的形态学改变, 油红"O"染色检测肝脏的脂质沉积。各组取血清检测同型半胱氨酸 (Hcy) 含量, 免疫组化和Western blot检测肝脏组织内亚甲基四氢叶酸还原酶 (MTHFR) 和胱硫醚合成酶 (CBS) 蛋白表达的变化.结果与正常对照组比较, 模型对照组空腹TG和TC浓度增加 (P<0.05) ;HE染色显示肝细胞排列紊乱, 出现脂肪变性;油红“O”染色显示肝脏组织的脂质沉积增加.与正常对照组比较, 模型对照组的Hcy的浓度增加 (P<0.05) , 人参皂苷Rg1治疗后降低 (P<0.05) , 存在剂量依赖性;与正常对照组比较, 模型对照组的MTHFR和CBS的蛋白质在肝脏组织中表达降低 (P<0.05) .人参皂苷Rg1治疗后, MTHFR和CBS的蛋白质表达升高 (P<0.05) , 存在剂量依赖性.结论在NAFLD模型中, 人参皂苷Rg1可能通过增加肝脏组织的MTHFR和CBS表达, 降低同型半胱氨酸的含量, 从而对非酒精性脂肪肝病发挥保护作用.
- 非酒精性脂肪肝病 /
- 人参皂苷Rg1 /
- 同型半胱氨酸 /
- 亚甲基四氢叶酸还原酶 /
- 胱硫醚合成酶
Abstract: Objective To establish animal model of non-alcoholic fatty liver disease (NAFLD) and explore the molecular mechanism of homocysteine changes in SD rats with NAFLD by ginsenoside Rg1 treating. Me thods NAFLD animal model was established to determine serum triglyceride and total cholesterol in the fasting state. HE staining was used to detect the morphological changes of the liver, and lipidosis of the liver tissue was observed by the oil red "o" staining in the control group and model control group. The serous concentration of homocysteine wasmeasured in every group by ELISA. Immunohistochemistry and Western blot were used to detect the expression levels of methylenetetrahydrofolate reductase (MTHFR) and cystathionine synthetase (CBS) in liver tissues. Results The concentrations of triglyceride and total cholesterol in the model control group were disordered compared with the control group (P<0.05) , meanwhile the morphology of the liver tissue was disordered and lipidosis was obvious in the model control group. So those results indicated the NAFLD model was successful. The concentration of homocysteine in the model control group was increased and decreased by Ginsenoside Rg1 treating (P<0.05) . The protein expressions of MTHER and CBS in model control group were lower compared with the control group by immunohistochemistry and Western blot (P<0.05) . The protein expressions ofMTHFR and CBS in the ginsenoside Rg1 treatment group were higher compared with the model control group by immunohistochemistry and Western blot (P<0.05) . The results have dose-dependent. Conclusion Ginsenoside Rg1 is possible to increase the protein expressions of MTHFR and CBS and decrease the concentration of homocysteine in animal models of non-alcoholic fatty liver, thus it can play a protective effect in non-alcoholic fatty liver.
- NAFLD /
- Ginsenoside Rg1 /
- Homocysteine /
- MTHFR /
- CBS

HTML全文

参考文献(17)

[1]	[1]CABALLERIA L, PERA G, RORDRIGUEZ L, et al.Metabolic syndrome and nonalcoholic fatty liver disease in a Spanish population influence of the diagnostic criteria used[J].Eur J Gastroenterol Hepatol, 2012, 24 (9) :1007-1011.
[2]	[2]ISSA D, PATEL V, SANYAL A J.Future therapy for non-alcoholic fatty liver disease[J].Liver Int, 2018, 38 (Suppl 1) :56-63.
[3]	[3]ALBA L M, LINDOR K L.Non-alcoholic fatty liver disease[J].Alimentary Pharmacology&Therapeutics, 2003, 17 (8) :977-986.
[4]	[4]HU Y, LIU J, DONG X, et al.Clinical study of serum homocysteine and non-alcoholic fatty liver disease in euglycemic patients[J].Med Sci Monit, 2016, 22 (1) :4146-4151.
[5]	[5]KASAPOGLU B, TURKAY C, YALCIN K S, et al.MTHFR677C/T and 1298A/C mutations and non-alcoholic fatty liver disease[J].Clin Med (Lond) , 2015, 15 (3) :248-251.
[6]	[6]NIU S, WANG L, HE M, et al.Exendin-4 regulates redox homeostasis in rats fed with high-fat diet[J].Acta BiochimBiophys Sin (Shanghai) , 2015, 47 (6) :397-403.
[7]	[7] LINDSEI K, SARNA, YAW L, et al.The CBS/CSE system:a potential therapeutic target in NAFLD[J].Physiol Pharmacol, 2015, 93 (1) :1-11.
[8]	[8] YOUNOSSIZM, KOENIGAB, ABDELATIFDF, et al.Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes[J].Hepatology, 2016, 64 (1) :73-84.
[9]	[9]DAY C P, JAMES O F.Steatohepatitis:A tale of two"hits"[J].Gastroenterology, 1998, 114 (4) :842-845.
[10]	[10]FOURET G, GAILLET S, LECOMTE J.20-Week follow-up of hepatic steatosis installationand liver mitochondrial structure and activity and their interrelation in rats fed a high fat high fructose diet[J].Br J Nutr, 2018, 119 (4) :368-380.
[11]	[11]GUO X F, GAO J L, LI J M, et al.fat-1 mice prevent high fat plus high-sugar diet-induced non-alcoholic fatty liver disease[J].Food Funct, 2017, 8 (11) :4053-4061.
[12]	[12]FRANCO BROCHADO MJ, DOMENICI F A.Methylenetetrahydrofolatereductase gene polymorphism and serum homocysteine levels in nonalcoholic fatty liver disease[J].Ann Nutr Metab, 2013, 63 (3) :193-199.
[13]	[13]SARNA L K, SIOW Y L, O K.The CBS/CSE system:a potential therapeutic target in NAFLD[J].Can J Physiol Pharmacol, 2015, 93 (1) :1-11.
[14]王雅楠, 李治纲, 张鹏, 等.高同型半胱氨酸血症中肝脏的脂肪变性[J].昆明医科大学学报, 2012, 33 (11) :19-22.
[15]	[15]ZONG Y, AI Q L, ZHONG L M, et al.Ginsenoside Rg1 attenuates lipopolysaccharide-induced inflammatory responses via the phospholipase C-γ1 signaling pathway in murine BV-2 microglial cells[J].Curr Med Chem, 2012, 19 (5) :770-779.
[16]	[16]GENG J, PENG W, HUANG Y, et al.Ginsenoside-Rg1from Panaxnotoginseng prevents hepatic fibrosis induced by thioacetamide in rats[J].European Journal of Pharmacology, 2010, 634 (1-3) :162-169.
[17]	[17]CHEN Z, LI C, YANG C, et al.Lipid regulation effects of raw and processed notoginseng radix et rhizome on steatotic hepatocyte L02 cell[J].Biomed Res Int, 2016, 2016 (29) :2919034.

相关文章(20)

[1]	门欣怡, 赵静, 申永椿, 季辉, 王秀霞. 外周血免疫球蛋白、血沉、同型半胱氨酸与儿童中枢神经系统血管炎病情程度的关系及对预后的影响, 昆明医科大学学报.
[2]	刘燃, 林志, 杨帆, 段继坤. 人参皂苷Rg1通过Sestrin2保护心肌细胞的作用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230614
[3]	李柳, 张晓婵, 茹琪, 王硕, 戚国庆, 赵红亮. 四氢叶酸还原酶基因多态性与冠心病严重程度的相关性, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210525
[4]	邓苙, 方红丽, 曹光琼, 高慧芳. 健康人群同型半胱氨酸与血脂的水平关系, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210107
[5]	曹光琼, 邓苙, 方红丽, 高慧芳, 奎帅. 用纵向队列法分析昆明地区体检人群同型半胱氨酸对血压、血糖及血脂的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210603
[6]	皮婷, 梁月琴, 丁莉, 杨玲, 向盈盈. 妊娠期女性MTHFR 677C > T基因多态性分布特征及其与妊娠高血压疾病的相关性, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210215
[7]	阮愕舒, 王烁, 苏惠, 尹凤琼, 钱忠义, 杨建宇. 人参皂苷Rg1对2型糖尿病大鼠的肾脏保护作用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210625
[8]	王礴, 夏咸松, 李进涛, 段小花. 细胞色素P450 2E1在甘草甜素治疗非酒精性脂肪肝病中的作用, 昆明医科大学学报.
[9]	朱为梅, 华鹏, 罗宇超, 杨红玲, 李亚山, 赵红宇. 低铅染毒抑制非酒精性脂肪性肝病大鼠脂肪酸分解加重其肝脏损伤, 昆明医科大学学报.
[10]	张梅. 高同型半胱氨酸血症对2型糖尿病患者血糖控制的影响, 昆明医科大学学报.
[11]	张红珊. 原发性高血压患者血Hcy与踝-臂脉搏波传导速度和颈动脉粥样硬化的相关性, 昆明医科大学学报.
[12]	刘师节. 老年冠心病患者冠脉病变和血浆同型半胱氨酸水平的关系, 昆明医科大学学报.
[13]	蔡亚梅. 无症状性脑梗死认知障碍与Hcy和hs-CRP的关系, 昆明医科大学学报.
[14]	张媛. 2型糖尿病合并非酒精性脂肪肝病患者Chemerin与氧化应激变化及临床意义, 昆明医科大学学报.
[15]	柴雁菁. 同型半胱氨酸对葡萄糖代谢和骨骼肌p-Akt（Ser-473)的影响, 昆明医科大学学报.
[16]	陶红飞. 高血压患者血液同型半胱氨酸值与性别的相关性分析, 昆明医科大学学报.
[17]	王雅楠. 高同型半胱氨酸血症中肝脏的脂肪变性, 昆明医科大学学报.
[18]	. TRB3在同型半胱氨酸抑制内皮细胞增殖中的作用研究, 昆明医科大学学报.
[19]	. 乙型肝炎患者氨基酸代谢与同型半胱氨酸浓度变化分析, 昆明医科大学学报.
[20]	. 血清同型半胱氨酸与冠脉病变的相关性研究, 昆明医科大学学报.