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人体混合感染巨片吸虫和巨片吸虫与肝片吸虫的中间型

张勇 白庭富 庄杉杉 李树德 田明 王文林

张勇, 白庭富, 庄杉杉, 李树德, 田明, 王文林. 人体混合感染巨片吸虫和巨片吸虫与肝片吸虫的中间型[J]. 昆明医科大学学报, 2018, 39(04): 40-46.
引用本文: 张勇, 白庭富, 庄杉杉, 李树德, 田明, 王文林. 人体混合感染巨片吸虫和巨片吸虫与肝片吸虫的中间型[J]. 昆明医科大学学报, 2018, 39(04): 40-46.
Zhang Yong , Bai Ting Fu , Zhuang Shan Shan , Li Shu De , Tian Ming , Wang Wen Lin . Faschiola gigantica and Intermediate Form between Faschiola gigantica and Faschola hepatica from a Mixed Infected Body[J]. Journal of Kunming Medical University, 2018, 39(04): 40-46.
Citation: Zhang Yong , Bai Ting Fu , Zhuang Shan Shan , Li Shu De , Tian Ming , Wang Wen Lin . Faschiola gigantica and Intermediate Form between Faschiola gigantica and Faschola hepatica from a Mixed Infected Body[J]. Journal of Kunming Medical University, 2018, 39(04): 40-46.

人体混合感染巨片吸虫和巨片吸虫与肝片吸虫的中间型

基金项目: 

基金: 国家自然科学基金资助项目 (81360252, 81360128); 云南公共卫生与疾病防控协同创新中心基金资助项目 (2014YNPHXT01, 2016YNPHXT05);

Faschiola gigantica and Intermediate Form between Faschiola gigantica and Faschola hepatica from a Mixed Infected Body

Funds: 

基金: 国家自然科学基金资助项目 (81360252, 81360128); 云南公共卫生与疾病防控协同创新中心基金资助项目 (2014YNPHXT01, 2016YNPHXT05);

  • 摘要: 目的 通过线粒体nad1和细胞核ITS1序列, 对从云南省玉溪地区一女性片形吸虫病患者体内取出的2条片形吸虫成虫进行虫种鉴定, 获知寄生于人体的片形吸虫种类.方法 片形吸虫标本保存于75%乙醇溶液, 提取全基因组DNA, PCR扩增线粒体基因NADH脱氢酶亚基1 (nad1) 和细胞核r DNA的第一内转录间隔区 (ITS1) 序列, 正反引物双向测序.所测序列与Gen Bank中Fasciola属相关同源序列组成DNA数据集, 进行ITS1单倍型分析和构建nad1的NJ系统发育树.结果测得片形吸虫标本的ITS1和nad1序列长度分别是422 bp和542bp.2条片形吸虫标本的nad1序列完全一致, 属于F.gigantica (Fg型) .但是ITS1单倍型不一致, 标本YXGD01在该基因中属于F.gigantica (Fg型) , 标本YXGD02属于F.hepatica (Fh型) .基于nad1 DNA数据集构建的NJ系统发育树, 明显分为F.gigantica和F.hepatica 2个类群.结论 基于线粒体nad1和细胞核ITS1序列, 从云南省玉溪地区一片形吸虫病患者体内取出的2条片形吸虫属于不同类型, 标本YXGD01鉴定为F.gigantica, 标本YXGD02可能是F.gigantica与F.hepatica杂交生成的中间型个体, 但需要更多的实验证据.
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  • 收稿日期:  2018-01-19

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