UPLC-MS/MS法研究滇乌碱在大鼠体内组织的分布

卢发焕; 李继印; 李树华; 赵文嵩; 王蕊; 解润芳; 杨凯润

UPLC-MS/MS法研究滇乌碱在大鼠体内组织的分布

1. 腾冲市公安局
2. 昆明医科大学法医学院
3. 云南省公安厅刑侦总队

基金项目:

基金：云南省科技计划基金资助项目 (2013FB118); 昆明医科大学科技创新团队建设基金资助项目 (CXTD201604); 云南省教育厅科学研究基金资助项目 (2017z DX161);

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出版历程
- 收稿日期: 2018-03-13

Tissue Distribution of Yunaconitine in Rats by UPLC-MS/MS Method

Funds:

基金：云南省科技计划基金资助项目 (2013FB118); 昆明医科大学科技创新团队建设基金资助项目 (CXTD201604); 云南省教育厅科学研究基金资助项目 (2017z DX161);

摘要

摘要: 目的建立灌胃给药滇乌碱的大鼠中毒模型, 用高效液相色谱-串联质谱 (UPLC-MS/MS) 法测定大鼠血液及各组织中滇乌碱的含量, 研究滇乌碱在大鼠体内的分布情况.方法将SD大鼠随机分为3组, 分别按2.2 mg/kg, 1.1 mg/kg和0.7 mg/kg剂量灌胃高、中、低剂量组大鼠, 给药2 h后处死, 迅速采取大鼠的心血、胃、小肠、肝脏、胰腺、肾脏、肺脏、脾脏、心脏、膀胱、睾丸和全脑, 液-液萃取法处理后, 用UPLC-MS/MS法测定各生物检材中滇乌碱的含量.结果滇乌碱大鼠中毒模型给药剂量为1.1 mg/kg, 滇乌碱在大鼠体内分布由高到低的顺序为胃、小肠、肝脏、胰腺、肾脏、肺脏、脾脏、心脏、膀胱、睾丸、心血和大脑.结论滇乌碱在大鼠体内分布广泛, 尤以胃、小肠和肝脏中含量最高, 该结论为黄草乌中毒的检验材料选取提供依据.
- 黄草乌 /
- 滇乌碱 /
- UPLC-MS/MS /
- 大鼠 /
- 体内分布
Abstract: Objective To establish an acute yunaconitine poisoning rat model with a single oral administration and to determine the contents of yunaconitine in rat tissues by UPLC-MS/MS method, then investigate the distribution of yunaconitine in rats. Me thod The rats were randomly divided into three groups and were intragastrically administered a single dose of 2.2 mg/kg, 1.1 mg/kg, 0.7 mg/kg yunaconitine, respectively.. The rats were killed 2 h later, the stomach tissue, intestine tissue, liver tissue, pancreas tissue, kidney tissue, lung tissue, spleen tissue, heart tissue, bladder tissue, testis tissue, brain tissue and heart blood samples were collected. The contents of yunaconitine in the biological materials were determined by UPLC-MS/MS method after the biological samples extracted by liquid-liquid extraction. Re s ult A rat model of the yunaconitine poisoning was made with a single dose of 1.1 mg/kg, the concentrations of yunaconitine displayed in the organs with the following order:stomach, small intestine, liver, pancreas, kidney, lung, spleen, heart, bladder, testis, heart blood and brain. Conclus ion Yunaconitine was widely distributed in rats, especially the levels in the stomach, small intestine and liver were the highest. The conclusion provides a basis for the selection of test materials for the poisoning of Aconitum vilmorinianum Kom.
- Aconitum vilmorinianum Kom /
- Yunaconitine /
- UPLC-MS/MS /
- Rat /
- Tissue distribution

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参考文献(18)

[1]李继印, 洪仕君, 黄舒柏清, 等.UPLC-MS/MS法检测黄草乌中毒人体肝脏中的主要生物碱成分[J].河南科技大学学报 (医学版) , 2017, 35 (4) :306-309.
[2]	[2]LANTANG, LINGYE, CHANGLV, et al.Involvement of CYP3A4/5 and CYP2D6 in the metabolism of aconitine using human liver microsomes and recombinant CYP450 enzymes[J].Toxicology Letters, 2011, 202 (1) :47-54.
[3]	[3]LINGYE, TAOWANG, CAIHUAYANG, et al.Microsomal cytochrome P450-mediated metabolism of hypaconitine, an active and highly toxic constituent derived from Aconitum species[J].Toxicology Letters, 2011, 204 (1) :81-91.
[4]	[4]XUE FAN, SHAN-SHAN YIN, XUE-JING LI, et al.Hydroxylation metabolisms of crassicauline a in rats under toxic dose[J].European Journal of Drug Metabolism and Pharmacokinetics, 2017, 42 (5) :857-869.
[5]李继印, 李绍鹏, 张瑞林, 等.UPLC-MS/MS法同时测定云南黄草乌中滇乌碱、粗茎乌头碱甲和塔拉萨敏的含量[J].云南大学学报:自然科学版, 2017, 39 (4) :650-655.
[6]张荣平, 陈泗英, 周俊.滇乌碱结构改造[J].云南植物研究, 1998, 20 (4) :474-478.
[7]郭志俊, 杨竹雅, 谭文红, 等.制黄草乌化学成分研究[J].中药材, 2015, 38 (5) :988-991.
[8]海青山, 马晓霞, 杨榆青, 等.滇西乌头中三种二萜生物碱相关药效和毒性的对比[J].昆明医科大学学报, 2017, 38 (1) :18-22.
[9]郭志俊, 段小花, 陈翠玲, 等.制黄草乌中滇乌碱及其转化产物8-去乙酰基滇乌碱药效和毒性的初步研究[J].中国中医药信息杂志, 2015, 22 (10) :60-63.
[10]	[10]YANG C, ZHANG T, LI Z, et al.P-glycoprotein is responsible for the poor intestinal absorption and low toxicity of oral aconitine:In vitro, in situ, in vivo and in silico studies[J].Toxicology and Applied Pharmacology, 2013, 273 (3) :561-568.
[11]	[11]YE L, YANG X, YANG Z, et al.The role of efflux transporters on the transport of highly toxic aconitine, mesaconitine, hypaconitine, and their hydrolysates, as determined in cultured Caco-2 and transfected MDCKII cells[J].Toxicology Letters, 2013, 216 (2-3) :86-99.
[12]熊耀嘉, 字淑慧, 吴静, 等.HPLC法同时测定直缘乌头中草乌甲素及滇乌碱的含量[J].中国药师, 2013, 16 (10) :1463-1465.
[13]	[13]ZHANG X, WANG X, LIU S, et al.HPLC determination four esteric akaloids from aconitun sungpanense hand.-Mazz[J].Medicinal Plant, 2014, 5 (8) :15-22.
[14]赫伟, 马晓霞, 张彬若, 等.HPLC法同时测定长喙乌头中3种主要二萜类生物碱含量[J].药物分析杂志, 2015, (10) :1829-1832.
[15]	[15]SONG L, ZHANG H, LIU X, et al.Rapid determination of yunaconitine and related alkaloids in aconites and aconite-containing drugs by ultra high-performance liquid chromatography-tandem mass spectrometry[J].BiomedChromatogr, 2012, 26 (12) :1567-1574.
[16]	[16]CHI KONG LAI, WING TAT POON, YAN WO CHAN.Hidden aconite poisoning:Identification of yunaconitine and related aconitum alkaloids in urine by liquid chromatography-tandem mass spectrometry[J].Journal of Analytical Toxicology, 2006, 30 (7) :426-433.
[17]	[17]NG S W, CHING C K, CHAN A Y W, et al.Simultaneous detection of 22 toxic plant alkaloids (aconitum alkaloids, solanaceous tropane alkaloids, sophora alkaloids, strychnos alkaloids and colchicine) in human urine and herbal samples using liquid chromatography-tandem mass spectrometry[J].Journal of Chromatography B, 2013, 942-943 (24) :63-69.
[18]	[18]ZHANG P, ZHANG F, WANG Z, et al.Simultaneous determination of four trace aconitum alkaloids in urine using ultra performance liquid chromatography-mass spectrometry[J].Se Pu, 2013, 31 (3) :211-217.

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