miR-223-3p在不同分期的糖尿病肾脏疾病血浆中的表达差异及意义

何君一; 李斓; 李嵘; 黄云华; 杨秋萍; 吴亚楠; 张丽华

miR-223-3p在不同分期的糖尿病肾脏疾病血浆中的表达差异及意义

1. 昆明医科大学第一附属医院老年内分泌科
2. 昆明医科大学第一附属医院糖尿病科
3. 云南省第一人民医院肾内科
4. 昆明医科大学第一附属医院内分泌科一科

基金项目:

基金：国家自然科学基金资助项目 (81560126); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2017FE468 (-042) ]; 云南省应用基础研究基金资助项目 (2016FB127); 成都医学院科研基金资助项目 (CYZ15-08);

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出版历程
- 收稿日期: 2018-02-23

Expression and Significance of Plasma miR-223-3p in Diabetic Kidney Diseaseat Different Stages

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摘要

摘要: 目的探索mi R-223-3p在糖尿病肾脏疾病 (DKD) 患者血浆中的表达情况及其临床意义.方法应用实时荧光定量PCR技术 (q RT-PCR) 分别检测糖尿病无肾病组60例 (DM组) 、糖尿病早期肾病组60例 (Micro-DKD组) , 糖尿病临床期肾病组60例 (Macro-DKD) 和正常健康对照组60例 (Control组) 血浆中mi R-223-3p的表达变化, 分析其与糖尿病肾脏疾病的关系;运用基因预测分析软件预测mi R-223-3p靶基因.结果与Control组相比, 其余3组患者血浆中mi R-223-3p的表达水平明显下降 (P<0.001) , 随着病情严重程度的进展, 血浆中mi R-223-3p的表达水平下降更加明显.生物信息学分析得出IL6ST和PRKCE为mi R-223-3p的靶基因.结论 mi R-223-3p表达水平在DKD患者血浆中表达下降并与严重程度有关, 可能通过调控其下游靶基因在糖尿病肾脏疾病发生过程中起重要作用.
- miR-223-3p /
- 糖尿病肾脏疾病 /
- 实时荧光定量PCR /
- 靶基因
Abstract: Objective To explore the expression of miR-223-3 p in the plasma of patients with diabetic kidney disease and its clinical significance. Me thods The expression levels of plasma miR-223-3 p in normoalbuminuric group (DM) , microoalbuminuria group (Micro-DKD) , macroalbuminuria group (Macro-DKD) and healthy controls were measured by quantitative real-time polymerase chain reaction (q RT-PCR) . To analysis the relationship with clinical pathological parameters, target genes of miR-223-3 p were predicted with bioinformatics software. Re s ults The levels of miR-223-3 p in the plasma of the remaining three groups were significantly lower than those in the healthy controls (P <0.001) , and the decrease was positively correlated with the severity of the disease. The potential target genes of miR-223-3 p identified by bioinformatics softwares include IL6 ST and PRKCE. Conclus ion The expressionof miR-223-3 pin diabetic kidney disease (DKD) patients' plasma decreased and was positively correlated with the severity of the disease. It may play an important role in the development and progression of diabetic kidney disease through its target genes.
- Diabetic kidney disease /
- miR-223-3p /
- Target genes /
- Quantitative real-time PCR

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