印记基因H19在不同质量精子和胚胎中的甲基化状态

相立峰; 王青青; 李明颖; 曾鹏; 李永刚

印记基因H19在不同质量精子和胚胎中的甲基化状态

云南省第一人民医院生殖医学科

基金项目:

基金：云南省卫生科技计划基金资助项目 (2014NS255);

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出版历程
- 收稿日期: 2018-04-12

A Study on Methylation Status of Imprinted Gene H19 and PEG1 in Different Quality of Sperms and Embryos

Funds:

基金：云南省卫生科技计划基金资助项目 (2014NS255);

摘要

摘要: 目的对印记基因H19在三种不同质量的精子中的甲基化状态以及精卵结合后形成胚胎的甲基化状态进行比较, 初步探索精子甲基化状态与受精后胚胎甲基化状态的关系.方法收集在云南省第一人民医院生殖医学科行IVF-ET治疗中废弃的精子91份和D3期低质量胚胎171份, 依据WHO第5版根据精子参数将精子样本分为3组, 分别分析3组的精子及受精后胚胎H19的甲基化状态.结果 B组在精子中和胚胎中H19甲基化状态异常比例显著高于另外2组 (P<0.05) .结论印记基因H19甲基化状态异常主要集中在异常参数精子组中, 提示其异常状态与精子质量降低有关;通过IVF、ICSI受精后的胚胎都出现同等比例的异常状态, 没有显著差异;异常精子的胚胎未出现异常, 这可能与胚胎自身的自我修复功能有关;而个别正常精子受精后的胚胎出现甲基化印记异常的原因可能与体外培养条件有关, 也有可能与卵子质量本身有关.
- 印记基因H19 /
- 精子 /
- 胚胎 /
- 甲基化 /
- 辅助生殖
Abstract: Objective By comparing the methylation status of imprinted gene H19 in three different quality spermatozoa and the methylation status of the embryos after the combination of spermatozoa, the sperm methylation status in the ART technique was preliminarily explored with the postnatal embryo methylation.Me thods In this study, a total of 91 spermatozoa and 91 low-quality embryos were collected from IVF-ET in the First People's Hospital of Yunnan Province. Among them, sperm samples were divided into three groups according to the sperm concentration parameter in the WHO fourth edition, then the relationship between different quality spermatozoa and postnatal embryo methylation were analyzed.Re s ults The abnormality rate of H19 methylation status in group B was significantly higher than that of the sperm group and the embryo group.Conclus ions The abnormalities of H19 methylation mainly concentrated in the abnormal parameters of spermatozoa, suggesting that the abnormal state was related to the decrease of sperm quality;Through IVF, ICSI fertilized embryos have the same proportion of the corresponding abnormal state with no significant difference; Abnormal sperm embryos did not show abnormality, which may be related to the self-repair function of the embryo itself.
- Imprinting gene H19 /
- Sperm /
- Embryo /
- Methylation /
- ART

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参考文献(12)

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