人参皂苷Rg3对体外培养小鼠神经干细胞增殖的影响

张凤兰; 赵瑜; 袁婧; 杨璐军; 鲁琳

人参皂苷Rg3对体外培养小鼠神经干细胞增殖的影响

张凤兰¹,
赵瑜²,
袁婧^1,3,
杨璐军⁴,
鲁琳^1,5

1. 昆明医科大学分子临床医学研究院云南省干细胞和再生医学重点实验室
2. 昆明医科大学生物医学工程研究中心
3. 云南省肿瘤医院病理科
4. 深圳市宝安区人民医院
5. 云南大学生命科学院

基金项目:

基金：云南省教育厅科学研究基金资助项目 (2014C039Y, 2016ZDX041);

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出版历程
- 收稿日期: 2018-02-24

Effect of Ginsenoside Rg3 on Mouse Neural Stem Cells Proliferation in Vitro

Zhang Feng Lan ¹,
Zhao Yu ²,
Yuan Jing ^1,3,
Yang Lu Jun ⁴,
Lu Lin ^1,5

Funds:

基金：云南省教育厅科学研究基金资助项目 (2014C039Y, 2016ZDX041);

摘要

摘要: 目的研究人参皂苷Rg3对体外培养小鼠神经干细胞 (NSCs) 增殖的影响.方法分离培养胎鼠NSCs, 使用神经球悬浮培养法传代, 特异性抗体Nestin和Sox2鉴定NSCs.采用Brd U标记法和CCK-8法检测不同浓度Rg3 (20 n M、50 n M、250 n M和500 n M) 对NSCs增殖的影响.结果传代2次后的神经球几乎全为Nestin/Sox2免疫荧光双阳性细胞, 表明其为纯度较高的NSCs.Brd U标记法表明与对照组、20 n M组、250 n M组和500 n M组相比, 50 n MRg3组Brd U阳性细胞相对比率最高, 差异有统计学意义 (P<0.01) , 但其他各组之间差异无统计学意义 (P>0.05) .CCK-8法检测, 结果显示50 n M Rg3在450 nm处吸光值最高, 且与对照组 (P<0.01) 、20 n M组 (P<0.05) 和500 n M组相比, 差异有统计学意义 (P<0.01) , 但其他各组之间无统计学差异 (P>0.05) .结论人参皂苷Rg3能促进体外培养小鼠NSCs的增殖, 且其最佳浓度是50 n M.
- 神经干细胞 /
- 人参皂苷Rg3 /
- 增殖 /
- BrdU /
- CCK-8
Abstract: Objective To investigate the effect of ginsenoside Rg3 on mouse neural stem cells (NSCs) proliferation in vitro.Me thods Fetal cortices of embryonic 14 d (E14) C57 BL/6 mouse were isolated for culturing primary NSCs.After being passaged twice, NSCs specific antibodies, Nestin and Sox2 were identified by immunocyto chemistry staining. CCK-8 assay and Brd U labelling assay were used to measure NSCs proliferation in response to different ginsenoside Rg3 concentrations (20 n M, 50 n M, 250 n M and 500 n M) . Re s ults Primary and passaged NSCs were successfully cultured and almost all the cells were both Nestin and Sox2 positive NSCs, which indicated high purity of NSCs. Brd U labelling assay showed the group of 50 n M Rg3 had the highest relative ratio of Brd U positive percentage, and the difference with other groups was statistically significant (P<0.01) .CCK-8 assay also showed 50 n M Rg3 group had the highest OD value, which was significantly higher than the control group (P<0.01) , 20 n M group (P<0.05) and 500 n M group (P<0.01) , while other groups had no significant difference between each other (P >0.05) . Conclus ion 50 n M ginsenoside Rg3 could significantly promote mouse NSCs proliferation in vitro.
- Neural stem cells /
- Ginsenoside Rg3 /
- Proliferation /
- BrdU /
- CCK-8

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参考文献(16)

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