甲基苯丙胺对大鼠海马5-HT、IL-6及NF-κB表达的影响

杨金凤; 蒙国懿; 黄兆奎; 洪仕君; 李利华; 邢豫明; 赵永娜

甲基苯丙胺对大鼠海马5-HT、IL-6及NF-κB表达的影响

1. 昆明医科大学药学院暨云南省天然药物药理重点实验室
2. 昆明医科大学法医学院
3. 云南省公安厅禁毒处毒品分析与禁毒技术公安部重点实验室
4. 昆明医科大学国际教育学院

基金项目:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB018);

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出版历程
- 收稿日期: 2018-02-12

Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus

Funds:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB018);

摘要

摘要: 目的研究甲基苯丙胺 (methamphetamine, METH) 对大鼠海马5-羟色胺 (5-Hydroxytryptophan、5-HT) 、白介素-6 (interleukin-6, IL-6) 及核因子-κB (nuclear factor kappa B, NF-κB) 的影响.方法将30只SD大鼠随机分为正常对照组、METH 1 d组、METH 3 d组、METH 5 d组、METH 7 d组、每组6只.分别在每天9:00和21:00腹腔注射METH[15 mg/ (kg·次) ];每次腹腔注射METH后, 结合刻板行为观察, 对各组进行评分;建立1 d、3 d、5 d、7 d组METH急性中毒大鼠模型.于末次给药24 h后分离海马组织, 应用酶联免疫吸附试验 (enzyme linked immunoorbent assay, ELISA) 检测实验大鼠海马5-HT、IL-6的含量;Western blot检测大鼠海马NF-κBp65的含量.结果与正常对照组比较, METH 1 d、3 d、5 d、7 d组大鼠海马5-HT含量均逐渐减少, 其中5 d组 (P<0.05) 和7 d组 (P<0.01) 差异具有统计学意义.与正常组对照组比较, METH 1 d、3 d、5d、7 d组大鼠海马IL-6的表达含量逐渐升高, 且5 d组 (P<0.01) 、7 d组 (P<0.01) 差异有统计学意义.NF-κB表达含量亦逐渐升高, 与正常组比较, 7 d组差异具有统计学意义 (P<0.01) .结论 METH可使大鼠海马5-HT含量下降、IL-6和NF-κB蛋白表达明显增加.
- 甲基苯丙胺 /
- 海马组织 /
- 5-HT /
- IL-6 /
- NF-κB
Abstract: Objective To study the effects of methamphetamine on the expression of 5-Hydroxytryptophan (5-HT) , interleukin-6 (IL-6) and nuclear fact kappa B (NF-κB) in rats' hippocampus. Methods Thirty SD rats were randomly allocated into normal control group, METH1 d group, METH3 d group, METH5 d group and METH7 d group, with 6 rats in each group.Intraperitoneal injections of METH [15 mg/ (kg·time) ] were conducted at9:00 am and 21:00 pm every day.After injection, scoring was performed in combination with observation on their stereotyped behavior. We established different models for rats suffering from 1-day, 3-day, 5-day, and 7-day METH acute poisoning. After 24 hours of the last intraperitoneal injections of METH, hippocampal tissues were isolated.Enzyme linked immunoorbent assay (ELISA) was applied to detect the expression level of 5-HT and IL-6 in rats hippocampus. Western blot was used to detect the expression level of NF-κBp65.Re s ults Compared with the control group, the expression of 5-HT was reduced in different METH groups, and the decrease was statistically significant in METH5 d and METH7 d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group, the expression of IL-6 was increased in different METH groups, and the increase was statistically significant in METH5 d and METH7 d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group, the expression of NF-κB was increased in different groups, and the increase was statistically significant only in METH7 d group (P <0.01) . Conclus ion METH administration could result in a significant reduce of 5-HT expression and increases of IL-6 and NF-κB expressions in rats hippocampus.
- Methamphetamine /
- Hippocampal tissue /
- 5-HT /
- IL-6 /
- NF-κB

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参考文献(18)

[1]李学峰, 丘平明, 等.甲基苯丙胺对相关脑区的神经毒性作用[J].解剖学杂志, 2007, 30 (1) :63-65.
[2]	[2]JOHN D, ROBINSON, CHRISTOPHER D, et al.Methamphetamine-induced neurotoxicity disrupts pharmacologically evoked dopamine transients in the dorsomedial and dorsolateral striatum[J].Neurotox Res, 2014, 26 (2) 152-167.
[3]	[3]AMANDA SIERRA, ASTRID E, CARDONA, et al.Functional diversity of microglia-how heterogenenous are they to begin with[J].Frontiers in Cellular Neuroscience, 2013, 14 (5) :1-18.
[4]	[4]BALDWIN A S.The NF-κB and IκB proteins:new discoveries and insights[J].Annu Rev Immunol, 1996, 14 (1) :649-683.
[5]	[5]KARIN M, BEN-NERIAH Y.Phosphorylation meets ubiquitination the control of NF-κB activity[J].Annu Rev Immunol, 2000, 18 (1) :621-663.
[6]	[6]RYANT, MOTT, MARTIANVENDRAME, et al.Neuronal expression of CD22:Novel nechanism for inhibiting microglial Proinflammatory cytokine production[J].GLIA, 2004, 46 (4) :369-379.
[7]	[7]SAMS-DODD F.Effects of continuous D-amphetamine and phencyclidine administration onsocial behaviour, stereotyped behaviour, and locomotor activity in rats[J].Neuropsychopha Rmacology, 1998, 19 (1) :18-25.
[8]	[8]THOMAS D M, DOWGIERT J, GEDDES T J, et al.Microglial activation is a pharmacologically specific marker for the neurotoxic amphetamines[J].Neurosci Lett, 2004, 367 (3) :349-354.
[9]	[9]GONCALVES J, RIBEIRO C F, MALVA J O, et al.Protective role of neuropeptide Y Y (2) receptors in cell death and microglial response following methamphetamine injury[J].Eur J Neurosci, 2012, 36 (9) :3173-3183.
[10]	[10]AIZENMAN E, MCCORD M C, SAADI R A, et al.Complex role of zinc in methamphetamine toxicity in vitro[J].Neuroscience, 2010, 171 (1) :31-39.
[11]	[11]MCCANN U D, KUWABARA H, KUMAR A, et al.Persistent cognitive and dopamine transporter deficits in abstinent methamphetamine users[J].Synapse, 2008, 62 (2) :91-100.
[12]苟红艳.CCK-8对甲基苯丙胺诱导神经损伤的影响及其抗炎机制研究[D].石家庄:河北医科大学, 2015.
[13]	[13]GONCALVES J, MARITINS T, MILHAZES N, et al.Methamphetmine-induced neuroinflammation of neuronal dysfunction in the mice hippocampus:preventive effect of indomethacin[J].Eur J Neurosci, 2010, 31 (2) :315-326.
[14]	[14]LEE Y W, HENNING B, YAO J, et al.Methamphetamine induces AP-1 and NF-kappa B binding and transanctivation in human brain endothedial cell[J].J Neurosci Res, 2001, 66 (4) :583-591.
[15]	[15]SHIFERA A S.The zinc finger domain of ikkgamma (Nemo) protein in health and disease[J].Cell Mol Med, 2010, 14 (10) :2404-2414.
[16]	[16]BAKER R G, HAYDEN M S, GHOSH S.NF-κB, Inflammation, and metabolic disease[J].Cell Metabolism, 2011, 13 (4) :11-22.
[17]	[17] MOTT R T, AIT-GHEZALA G, TOWN T, et al.Neuronal expression of CD22:novel mechanism for inhibiting microglial proinflammatory cytokine production.[J].Glia, 2004, 46 (4) :369-379.
[18]	[18]NAGATSU T.Parkinson.5 disease:changes in apoptosis-related faetors suggesting Possible gene therapy[J].Neural Transm, 2002, 109 (526) :731-745.

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