甲基苯丙胺对大鼠海马5-HT、IL-6及NF-κB表达的影响

杨金凤; 蒙国懿; 黄兆奎; 洪仕君; 李利华; 邢豫明; 赵永娜

甲基苯丙胺对大鼠海马5-HT、IL-6及NF-κB表达的影响

1. 昆明医科大学药学院暨云南省天然药物药理重点实验室
2. 昆明医科大学法医学院
3. 云南省公安厅禁毒处毒品分析与禁毒技术公安部重点实验室
4. 昆明医科大学国际教育学院

基金项目:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB018);

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出版历程
- 收稿日期: 2018-02-12

Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus

Funds:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB018);

摘要

摘要: 目的研究甲基苯丙胺 (methamphetamine, METH) 对大鼠海马5-羟色胺 (5-Hydroxytryptophan、5-HT) 、白介素-6 (interleukin-6, IL-6) 及核因子-κB (nuclear factor kappa B, NF-κB) 的影响.方法将30只SD大鼠随机分为正常对照组、METH 1 d组、METH 3 d组、METH 5 d组、METH 7 d组、每组6只.分别在每天9:00和21:00腹腔注射METH[15 mg/ (kg·次) ];每次腹腔注射METH后, 结合刻板行为观察, 对各组进行评分;建立1 d、3 d、5 d、7 d组METH急性中毒大鼠模型.于末次给药24 h后分离海马组织, 应用酶联免疫吸附试验 (enzyme linked immunoorbent assay, ELISA) 检测实验大鼠海马5-HT、IL-6的含量;Western blot检测大鼠海马NF-κBp65的含量.结果与正常对照组比较, METH 1 d、3 d、5 d、7 d组大鼠海马5-HT含量均逐渐减少, 其中5 d组 (P<0.05) 和7 d组 (P<0.01) 差异具有统计学意义.与正常组对照组比较, METH 1 d、3 d、5d、7 d组大鼠海马IL-6的表达含量逐渐升高, 且5 d组 (P<0.01) 、7 d组 (P<0.01) 差异有统计学意义.NF-κB表达含量亦逐渐升高, 与正常组比较, 7 d组差异具有统计学意义 (P<0.01) .结论 METH可使大鼠海马5-HT含量下降、IL-6和NF-κB蛋白表达明显增加.
- 甲基苯丙胺 /
- 海马组织 /
- 5-HT /
- IL-6 /
- NF-κB
Abstract: Objective To study the effects of methamphetamine on the expression of 5-Hydroxytryptophan (5-HT) , interleukin-6 (IL-6) and nuclear fact kappa B (NF-κB) in rats' hippocampus. Methods Thirty SD rats were randomly allocated into normal control group, METH1 d group, METH3 d group, METH5 d group and METH7 d group, with 6 rats in each group.Intraperitoneal injections of METH [15 mg/ (kg·time) ] were conducted at9:00 am and 21:00 pm every day.After injection, scoring was performed in combination with observation on their stereotyped behavior. We established different models for rats suffering from 1-day, 3-day, 5-day, and 7-day METH acute poisoning. After 24 hours of the last intraperitoneal injections of METH, hippocampal tissues were isolated.Enzyme linked immunoorbent assay (ELISA) was applied to detect the expression level of 5-HT and IL-6 in rats hippocampus. Western blot was used to detect the expression level of NF-κBp65.Re s ults Compared with the control group, the expression of 5-HT was reduced in different METH groups, and the decrease was statistically significant in METH5 d and METH7 d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group, the expression of IL-6 was increased in different METH groups, and the increase was statistically significant in METH5 d and METH7 d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group, the expression of NF-κB was increased in different groups, and the increase was statistically significant only in METH7 d group (P <0.01) . Conclus ion METH administration could result in a significant reduce of 5-HT expression and increases of IL-6 and NF-κB expressions in rats hippocampus.
- Methamphetamine /
- Hippocampal tissue /
- 5-HT /
- IL-6 /
- NF-κB

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参考文献(18)

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