组蛋白去乙酰化酶2在人舌鳞状细胞癌中的表达

李建娇; 代晓明

组蛋白去乙酰化酶2在人舌鳞状细胞癌中的表达

昆明医科大学第一附属医院整形外科

基金项目:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2013FB147, 2010CD187); 昆明医科大学硕士研究生创新科研基金资助项目 (2017S017);

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出版历程
- 收稿日期: 2018-03-19

A Study on Expression of Histone Deacetylase-2 in Humantongue Squamous Cell Carcinomas

Funds:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2013FB147, 2010CD187); 昆明医科大学硕士研究生创新科研基金资助项目 (2017S017);

摘要

摘要: 目的采用免疫组织化学染色的方法, 研究组蛋白去乙酰化酶2 (HDAC2) 蛋白在人舌正常粘膜, 不典型增生及鳞状细胞癌中的表达, 并分析其与患者的临床指标及生存状况的相关性.方法标本分为:舌鳞状细胞癌组 (n=96) 、舌粘膜不典型增生组 (n=24) 、正常舌粘膜组 (n=13) 行免疫组织化学染色, 阳性细胞占比≥5%为阳性.Kaplan-Merier生存分析法分析患者生存状况.结果 96例舌鳞状细胞癌样本中有86例为阳性表达 (89.6%) , HDAC2的过表达与患者年龄、性别、烟酒嗜好、病理分级、淋巴结转移等因素之间的差异无统计学意义 (P>0.05) , 但与癌症的TNM分期之间差异有统计学意义 (P<0.001) .24例不典型增生组织中有14例 (58.3%) 呈阳性表达, 正常舌粘膜组织中有7例 (53.8%) 阳性表达样本.HDAC2蛋白在舌鳞状细胞癌患者中的阳性表达率显著高于不典型增生组和正常舌粘膜组 (P<0.00) .HDAC2的表达与患者术后生存情况之间无相关性 (P=0.184) , 有淋巴结转移的患者术后生存时间明显短于无淋巴结转移的患者 (P<0.000) .结论 HDAC2在人舌鳞状细胞癌中的阳性表达率明显高于不典型增生组和正常舌粘膜组.HDAC2阳性表达的患者肿瘤分期级别较高, 并且有淋巴结转移者的术后生存时间明显低于无淋巴结转移者.提示HDAC2可作为舌鳞癌预后的一个可靠的分子参考指标.
- 组蛋白去乙酰化酶2 /
- 临床病理 /
- 舌鳞状细胞癌 /
- 免疫组化
Abstract: Objective To study the expression of HDAC2 and the correlations with pathological parameters in human tongue squamous cell carcinoma, and to analyze the correlation between them and patients' clinical indicators and survival status.Methods A total of 96 cases of humantongue squamous cell carcinoma (SCC) , 24 dysplasia (ED) , 13 normal mucosa (NOM) were involved in this study. The expression of HDAC2 was evaluated by ICH. Kaplan-Meier was used to research cumulative surviving of SCC patients.Result Among 96 cases of SCC samples, 86 cases showed positive expression (89.6%) , The differences between the overexpression of HDAC2 and the pathological grading, lymph node metastasis, the patient's age and gender were insignificant (all P>0.05) . However, the differences of TNM presented statistically significant (χ2 = 18.600, P<0.001) . 14 out of 24 ED (58.3%) were positive expression of HDAC2, there were 7 cases (53.8%) of positive expression samples in NOM. The expression of HDAC2 in SCCs was significantly higher than that in ED and NOM (χ2= 118.407, P<0.00) . There was no significant difference of cumulative surviving between the positive and negative SCC patients (P = 0.184) . However cumulative surviving of patients with lymph node metastasis was significantly shorter than those with no metastasis (P<0.000) .Conclusions The expression of HDAC2 in SCC was significantly higher than that in ED and NOM, the TNM stage of patients presented the significant difference of expression of HDAC2, patients with lymph node metastasis were significantly shorter than those with no metastasis, and demonstration can be used as a reliable reference molecule for the evaluating prognosis of SCC.
- Pathological parameters /
- Histone deacetylases-2 /
- Immunohisochemistry /
- Tongue squamous cell carcinoma

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参考文献(16)

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