Abstract: Objective To establish the methamphetamine induced rat conditioned place preference (CPP model, compare the behaviour effects of different time courses of methamphetamine administration and the intervention effect of gastrodin on rat. Methods The rats were intraperitoneally injected (ip) with 10 mg/kg of methamphetamine for 10 days;after confirming the CPP effects were established in rats, the animals of experimental groups were injected with 10 mg/kg of methamphetamine for 7 days, 14 days, and 28 days respectively, to establish three methamphetamine dependent rat model with different dependent times. And then, the gastrodin intervention groups were given 10 mg/kg of gastrodin for 28 days. Then, CPP test were conducted. Results Compared with the control group, the times spent in the drug-paired chamber were increasd significantly in groups of MA-dependent 7 days, 14 days, and 28 days. Moreover, there was statistical difference between 14 days group and 7 days group or 28 days group, but there was no statistical difference between 7 days group and 28 days group.There was significant difference between the MA+Gastrodin groups and the MA groups, and gastrodin intervention groups had shorter time in the drug-paired chamber. Conclusions Constant injection of methamphetamine for 7 days, l4 days, and 28 days can successfully induce CPP effects and establish MA dependent rat model. The CPP effect of methamphetamine 14 days group is more significant than other groups. Gastrodin inervention can alleviate the CPP effect of rat.