Beclin-1、LC3-Ⅱ在宫颈病变组织中的表达及意义
The Expression and Significance of Beclin-1 and LC3-Ⅱ in Cervical Lesions
-
摘要: 目的 检测Beclin-1、LC3-Ⅱ在宫颈病变组织及HPV感染中的表达, 并探讨其临床意义。方法选取昆明医科大学第二附属医院妇科2014年10月至2016年6月的行宫颈癌筛查 (HPV、TCT) 的患者, 筛查结果任意一项异常患者均行宫颈活检, 最终选取病检结果为正常的宫颈组织40例 (其中HPV感染20例, 无HPV感染20例) ;CINⅠ30例;CINⅡ/Ⅲ30例;年龄范围为25～55岁;妊娠及已行宫颈治疗过的患者不纳入研究范围。用Western Blot法检测正常宫颈组织、CINⅠ及CINⅡ/Ⅲ组织中Beclin-1、LC3-Ⅱ相对表达量。结果 (1) Beclin-1在正常的宫颈组织、CINⅠ及CINⅡ/Ⅲ组织中的相对表达量分别为 (0.561±0.041) 、 (0.421±0.018) 、 (0.351±0.016) ;LC3-Ⅱ在正常宫颈组织、CINⅠ及CINⅡ/Ⅲ组织中的相对表达量为 (0.683±0.027) 、 (0.435±0.029) 、 (0.325±0.017) ;Beclin-1、LC3-Ⅱ在3组间的相对表达量差异有统计学意义 (P <0.001) ;Beclin 1, LC3-Ⅱexist in the relative expression between the 3 group were statistically significant (P <0.001) (2) 病检结果为正常的宫颈组织中HPV感染组中Beclin-1和LC3-Ⅱ的相对表达量均显著低于无HPV感染组 (P <0.05) 。结论随着宫颈病变级别的增加, 自噬相关基因Beclin-1、LC3-Ⅱ的相对表达量呈下降趋势, 提示自噬基因活性下降或缺失可能参与宫颈癌的进展。Abstract: Objective To detect the expression and significance of Beclin-1 and LC3-Ⅱin cervical lesions and HPV infection, and explore their clinical significance. Methods We selected patients in the department of gynecology of the second affiliated hospital of kunming medical university form October 2014 to June 2016 to accept cervical cancer screening (HPV, TCT) , and if any result was abnormal, the patient should be given cervical biopsy. Finally, 40 cases of normal cervical tissues (including 20 cases of HPV infection and 20 cases of no HPV infection) were selected as the disease test result. CINⅠ30 cases; CINⅡ/Ⅲ30 cases; the age range from 25 to 55 years old. The patients who received cervical lesions treatment and pregnant women were not included in the study.Western Blot was used to detect the relative transcript level of Beclin-1, LC3-Ⅱin normal cervical tissues and CINⅠ, CINⅡ/Ⅲ. Results (1) The relative expression levels of Beclin-1 in normal cervical tissue and CIN Ⅰ and CIN Ⅱ/Ⅲ tissuses were (0.561±0.041) , (0.421±0.018) and (0.351±0.016) respectively;The relative expression levels of LC3-Ⅱ in normal cervical tissue and CIN Ⅰ and CIN Ⅱ/Ⅲ tissues were (0.683±0.027) , (0.435±0.029) and (0.325±0.017) . The difference in the relative expression of Beclin-1 and LC3-Ⅱ between the 3 groups were statistically significant (P < 0.001) .The relative expression of Beclin-1 and LC3-II in normal cervical tissues with HPV infection was significantly lower than that in normal cervical tissues without HPV infection (P < 0.05) . Conclusion With the increase of cervical lesion level, autophagy related genes Beclin-1, the relative expression of LC3-Ⅱ is on the decline, suggesting autophagy gene activity decline or loss may be involved in the progress of cervical cancer.
-
Key words:
- Cervical lesions /
- Autophagy /
- Beclin-1 /
- LC3-Ⅱ
-
[1] [1]SIEGEL R, MA J, ZOU et al.Cancer statistics, 2014[J].CA Cancer J Clin, 2014, 64 (1) :9-29. [2]胡幸, 符青云, 何凯宁, 等.P16和Ki-67免疫组化检测在宫颈上皮内病变病理诊断中的应用[J].广东医科大学学报, 2018, 36 (1) :61-64. [3] [3]ZHANG C, SYES T W, LIU R.Role of endoplasmic reticulum stress, autophagy, andinflammation in cardiovascular disease[J].Front Cardiovase Med, 2017, 4:29. [4] [4]WANG Y, WANG Y L, HUNANG X, et al.lbutilide protects against cardiomyocytes injury via inhabiting endoplamic reticulum and mitichondrial stress pathways[J].Heral Vwssels, 2016, 36 (1) :5-11. [5] [5]RUTTON RW, LUO S, RUBINSZTEIN DC. Autophagic activity in neuronal cell death[J].Neurosci Bull, 2015, 31 (4) :382-394. [6] [6]HAH Y S, KIM J G, CHO H Y, et al.Procyanidins from Vitis vinifera seeds induce apoptotic and autophagic cell death via generation of reacctive oxygen species in squamous cell carcinoma cells[J].Oncol Lett, 2017, 14 (2) :1925-1932. [7]郭亚男, 赵瑞红, 刘青.自噬与糖尿病肾病关系的研究进展[J].河北医科大学学报, 2016, 10 (4) :855-858. [8]邬琳, 郭金珠.EGFR和自噬标记蛋白Beclin1在宫颈癌组织中的表达及意义[J].实用癌症杂志, 2017, 7 (32) :1079-1081. [9]李秀丹, 石立力, 姜晓艳.血管平滑肌细胞自噬与糖尿病动脉粥样硬化关系的研究进展[J].临床心血管病杂志, 2019, 35 (1) :92-95. [10] [10]ZHOU GUO-HUA, YAO WEI-SHEN, ZHANG XU.Expression of Beclin1 and LC3 in the tissue of invasive ductal carcinoma[J].Hainan Med, 2013, 24 (5) :630-632. [11] [11]KUBISCH J, TU..REI D, F..O LDVA..RI-NAGY L, et al.Complex regulation of autophagy in cancer-integrated approaches to discover the networks that hold a double-edged sword[J].Semin Cancer Biol, 2013, 23 (4) :252-261. [12] [12]ZHONG Y, MORRIS D H, JIN L, et al.Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing beclin1-vps34 complex achitecture and reducing intracellular hosphatidylinositol-3 phoshpate levels[J].J Biol Chem, 2014, 289 (38) :26021-26037. [13] [13]BOUTOUJA F, BRINKMEIER R, MASTALSKI T, et al.Regulation of the tumor-suppressor BECLIN 1 by distinct ubiquitination cascades[J]. Int J Mol Sci, 2017, 18 (12) :E2541. [14]杨威, 杨红杰, 莫瑞祥, 等.Beclin1在人肝癌组织中的表达及TGF-β对肝癌细胞增殖的影响[J].国际检验医学杂志, 2015, 36 (1) :108-109. [15] [15]WILKINSON D S, JARIWALA J S, ANDERSON E, et al.Phosphorylation of LC3 by the Hippo kinases STK3/STK4 is essential for autophagy[J].Mol Cell, 2015, 57 (1) :55-68. [16]童焰, 谭布珍, 方玉婷, 等.细胞自噬在宫颈癌中的研究进展[J].解剖学报, 2017, 48 (1) :111-114. [17] [17]YAN HONG, WU ZHENG-SHENG, JIANG DONG-RUI, et al.Expression of autophagy-related gene LC3 and apoptosis-related gene XIAP in breast cancer and their significance[J].J Clin Exp Pathol, 2016, 32 (4) :409-412. [18]段昌虎, 刘晓晨, 豆发福, 等.HBx及Beclin1在肝癌中表达的关系研究[J].肝脏, 2017, 22 (1) :20-23. [19]王凯, 郑洋, 邢雅玲, 等.细胞自噬在病毒感染与免疫调节中的作用机制[J].中国生物化学与分子生物学报, 2011, 27 (11) :987-992. -
点击查看大图
计量
- 文章访问数: 2081
- HTML全文浏览量: 727
- PDF下载量: 123
- 被引次数: 0
下载:
