云南白药对人体富血小板纤维蛋白释放细胞生长因子的影响

王礼钰; 和红兵; 谭锦章; 任晓斌

云南白药对人体富血小板纤维蛋白释放细胞生长因子的影响

昆明医科大学附属口腔医院牙周病科

基金项目:

基金：国家自然科学基金资助项目 (8166040056); 云南省教育厅科学研究基金资助项目 (2016ZDX065); 昆明医科大学研究生创新基金资助项目 (2018S177);

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出版历程
- 收稿日期: 2018-11-13

Effect of Yunnan Baiyao on Cytokines Releasing from Platelet Rich-Fibrin in Human

Funds:

基金：国家自然科学基金资助项目 (8166040056); 云南省教育厅科学研究基金资助项目 (2016ZDX065); 昆明医科大学研究生创新基金资助项目 (2018S177);

摘要

摘要: 目的检测口服云南白药前后富血小板纤维蛋白 (PRF) 中血小板衍生生长因子-AB (PDGF-AB) 、转化生长因子-β1 (TGF-β1) 、类胰岛素生长因子 (IGF) 的浓度变化情况。方法口服云南白药前后, 抽取志愿者静脉血10 m L制备PRF膜, 分别于37℃下静置1 d, 7 d, 14 d, 21 d, 28 d, 收集其渗出液, 并用ELISA法检测渗出液浓度。结果 (1) 生长因子释放情况:在不同时间点PRF渗出液中, TGF-β1浓度趋于平稳;14 d时PDGF-AB、7 d时IGF浓度达峰值; (2) 口服云南白药后生长因子浓度显著高于用药前 (P<0.05) 。结论 PRF能持久缓慢地释放细胞生长因子, 在口服云南白药后, 能有效刺激PRF释放更高浓度的生长因子。
- 云南白药 /
- 富血小板纤维蛋白 /
- 生长因子
Abstract: Objective To observe the variation in PRF before and after taking yunnan baiyao by detecting the concentration of three kinds of cytokines which are Platelet Derived Growth Factors (PDGF-AB) , Transforming Growth Factors (TGF-β) , Insulin-like Growth Factors (IGF) . Methods There were 3 volunteers who were approved by institutional ethical. We collected the venous blood 10 m L from them, in order to prepare for the PRF membrane. Then we put the blood under 37 ℃ and collected their exudates on 1 d, 7 d, 14 d, 21 d, 28 d separately, and used ELISA method to detect the concentration of the exudates. Results (1) The situation of the cytokines releasing: at different time points, TGF-β1 concentration were sable;on day 14 the concentration of PDGF-AB reached the highest, and on day 7 concentration of IGF-1 reached the peak. 2) After taking yunnan baiyao the concentration of cytokines was higher than before observably (P < 0.05) .Conclusion PRF can release the cytokines constantly, taking the yunnan baiyao can stimulate the PRF to release higher concentration of cytokines effectively.
- Yunnan baiyao /
- Platelet rich-fibrin /
- Cytokines

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参考文献(17)

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