云南白药对人体富血小板纤维蛋白释放细胞生长因子的影响

王礼钰; 和红兵; 谭锦章; 任晓斌

云南白药对人体富血小板纤维蛋白释放细胞生长因子的影响

昆明医科大学附属口腔医院牙周病科

基金项目:

基金：国家自然科学基金资助项目 (8166040056); 云南省教育厅科学研究基金资助项目 (2016ZDX065); 昆明医科大学研究生创新基金资助项目 (2018S177);

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出版历程
- 收稿日期: 2018-11-13

Effect of Yunnan Baiyao on Cytokines Releasing from Platelet Rich-Fibrin in Human

Funds:

基金：国家自然科学基金资助项目 (8166040056); 云南省教育厅科学研究基金资助项目 (2016ZDX065); 昆明医科大学研究生创新基金资助项目 (2018S177);

摘要

摘要: 目的检测口服云南白药前后富血小板纤维蛋白 (PRF) 中血小板衍生生长因子-AB (PDGF-AB) 、转化生长因子-β1 (TGF-β1) 、类胰岛素生长因子 (IGF) 的浓度变化情况。方法口服云南白药前后, 抽取志愿者静脉血10 m L制备PRF膜, 分别于37℃下静置1 d, 7 d, 14 d, 21 d, 28 d, 收集其渗出液, 并用ELISA法检测渗出液浓度。结果 (1) 生长因子释放情况:在不同时间点PRF渗出液中, TGF-β1浓度趋于平稳;14 d时PDGF-AB、7 d时IGF浓度达峰值; (2) 口服云南白药后生长因子浓度显著高于用药前 (P<0.05) 。结论 PRF能持久缓慢地释放细胞生长因子, 在口服云南白药后, 能有效刺激PRF释放更高浓度的生长因子。
- 云南白药 /
- 富血小板纤维蛋白 /
- 生长因子
Abstract: Objective To observe the variation in PRF before and after taking yunnan baiyao by detecting the concentration of three kinds of cytokines which are Platelet Derived Growth Factors (PDGF-AB) , Transforming Growth Factors (TGF-β) , Insulin-like Growth Factors (IGF) . Methods There were 3 volunteers who were approved by institutional ethical. We collected the venous blood 10 m L from them, in order to prepare for the PRF membrane. Then we put the blood under 37 ℃ and collected their exudates on 1 d, 7 d, 14 d, 21 d, 28 d separately, and used ELISA method to detect the concentration of the exudates. Results (1) The situation of the cytokines releasing: at different time points, TGF-β1 concentration were sable;on day 14 the concentration of PDGF-AB reached the highest, and on day 7 concentration of IGF-1 reached the peak. 2) After taking yunnan baiyao the concentration of cytokines was higher than before observably (P < 0.05) .Conclusion PRF can release the cytokines constantly, taking the yunnan baiyao can stimulate the PRF to release higher concentration of cytokines effectively.
- Yunnan baiyao /
- Platelet rich-fibrin /
- Cytokines

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参考文献(17)

[1]	[1]CHOUKROUN J, DISS A, SIMONPIERI A, et al.Platelet-rich fibrin (PRF) :A second generation platelet concentrate. Part V:Histologic evaluations of Choukroun’s PRF effects on bone allograft maturation in sinus lift[J].Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2006, 101 (3) :299-303.
[2]	[2] KANAYAMA T, HORII K, SENGA Y, et al. Crestal approach to sinus floor elevation for atrophic maxilla using platelet-rich fibrin as the only grafting material:a 1-year prospective study[J].Implant Dent, 2016, 25 (1) :32-38.
[3]	[3]MOUSSA M, EL-DAHAB OA, EL NAHASS H. Anterior maxilla augmentation using palatal bone block with platelet rich fibrin:a controlled tria[J].Int J Oral Maxillofac Implants, 2016, 31 (3) :708-715.
[4]	[4] DAS S, JHINGRAN R, BAINS VK, et al. Socket preservation by beta-tri-calcium phosphate with collagen compared to platelet-rich fibrin:a clinic-radiographic study[J].Eur J Dent, 2016, 10 (2) :264-276.
[5]	[5]KANAYAMA T, HORII K, SENGA Y, et al. Crestal approach to sinus floor elevation for atrophic maxilla using platelet-rich fibrin as the only grafting material:a 1-year prospective study[J].Implant Dent, 2016, 25 (1) :32-38.
[6]	[6]MOUSSA M, EL-DAHAB OA, EL NAHASS H. Anterior maxilla augmentation using palatal bone block with platelet rich fibrin:a controlled trial[J].Oral Maxillofac Implants, 2016, 31 (3) :708-715.
[7]	[7]WAGNER W, WEHRMANN M.Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin[J].Cell Mol Med, 2007, 11 (6) :1342-1351.
[8]	[8]HAUSER F, GAYDAROV N, BADOUD L, et al. Clinical and histologyical evaluation of postextraction platelet-rich fibrin socket filling:a prospective randomized controlled study[J].Implant Dent, 2013, 22 (3) :295-303.
[9]	[9]DAS S, JHINGRAN R, BAINS VK, et al. Socket preservation by betatricalcium phosphate with collagen compared to platelet-rich fibrin:a clinic-radiographic study[J].Dent, 2016, 10 (2) :264-276.
[10]	[10]MARRELLI M, TATULLO M. Influence of PRF in the healing of bone and gingival tissues. Clinical and histological evaluations[J].Eur Rev Med Pharmacol Sci, 2013, 17 (14) :1958-1962.
[11]孙洁, 张剑明, 李彦秋, 富血小板纤维蛋白超微结构的观察与探讨[J].口腔医学研究, 2010, 26 (01) :98-101.
[12]张小梅, 莫治强, 李俊明, 等.云南白药对围手术期血小板表面糖蛋白及超微结构影响的研究[J].昆明医学院学报, 2004, 25 (2) :56-59.
[13]杨吉林, 莫志强, 罗用宇, 等.口服云南白药胶囊对骨科病人术中出血量影响的研究[J].云南医药, 2003, 46 (3) :175-178.
[14]唐正龙, 王兴, 伊彪, 等.云南白药对血小板的释放与代谢反应的影响[J].贵阳医学院学报, 2009, 34 (3) :282-285.
[15]	[15]GIANNINI S, CIELO A, BONANOME L, et al.Comparison between PRP, PRGF and PRF:Lights and shadows in three similar but different protocols[J].Europcan Review for Medical and Pharmacological Sciences, 2015, 19 (6) :927-930.
[16]	[16]DOHAN DM, CHOUKROUN J, DISS A, et al.Platelet-rich fibrin (PRF) :a second-generation platelet concentrate. Part II:platelet-related biologic features[J].Oral Surg Oral Med Oral Radiol Endod, 2006, 101 (3) :e51-55.
[17]白彭, 叶平.两种胶原膜暴露于El腔环境中降解作用的对照研究[J].中国口腔种植学杂志, 2011, 16 (1) :56-60.

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