云南白药对人体富血小板纤维蛋白释放细胞生长因子的影响

王礼钰; 和红兵; 谭锦章; 任晓斌

云南白药对人体富血小板纤维蛋白释放细胞生长因子的影响

昆明医科大学附属口腔医院牙周病科

基金项目:

基金：国家自然科学基金资助项目 (8166040056); 云南省教育厅科学研究基金资助项目 (2016ZDX065); 昆明医科大学研究生创新基金资助项目 (2018S177);

计量
- 文章访问数: 2323
- HTML全文浏览量: 889
- PDF下载量: 80
- 被引次数: 0
出版历程
- 收稿日期: 2018-11-13

Effect of Yunnan Baiyao on Cytokines Releasing from Platelet Rich-Fibrin in Human

Funds:

基金：国家自然科学基金资助项目 (8166040056); 云南省教育厅科学研究基金资助项目 (2016ZDX065); 昆明医科大学研究生创新基金资助项目 (2018S177);

摘要

摘要: 目的检测口服云南白药前后富血小板纤维蛋白 (PRF) 中血小板衍生生长因子-AB (PDGF-AB) 、转化生长因子-β1 (TGF-β1) 、类胰岛素生长因子 (IGF) 的浓度变化情况。方法口服云南白药前后, 抽取志愿者静脉血10 m L制备PRF膜, 分别于37℃下静置1 d, 7 d, 14 d, 21 d, 28 d, 收集其渗出液, 并用ELISA法检测渗出液浓度。结果 (1) 生长因子释放情况:在不同时间点PRF渗出液中, TGF-β1浓度趋于平稳;14 d时PDGF-AB、7 d时IGF浓度达峰值; (2) 口服云南白药后生长因子浓度显著高于用药前 (P<0.05) 。结论 PRF能持久缓慢地释放细胞生长因子, 在口服云南白药后, 能有效刺激PRF释放更高浓度的生长因子。
- 云南白药 /
- 富血小板纤维蛋白 /
- 生长因子
Abstract: Objective To observe the variation in PRF before and after taking yunnan baiyao by detecting the concentration of three kinds of cytokines which are Platelet Derived Growth Factors (PDGF-AB) , Transforming Growth Factors (TGF-β) , Insulin-like Growth Factors (IGF) . Methods There were 3 volunteers who were approved by institutional ethical. We collected the venous blood 10 m L from them, in order to prepare for the PRF membrane. Then we put the blood under 37 ℃ and collected their exudates on 1 d, 7 d, 14 d, 21 d, 28 d separately, and used ELISA method to detect the concentration of the exudates. Results (1) The situation of the cytokines releasing: at different time points, TGF-β1 concentration were sable;on day 14 the concentration of PDGF-AB reached the highest, and on day 7 concentration of IGF-1 reached the peak. 2) After taking yunnan baiyao the concentration of cytokines was higher than before observably (P < 0.05) .Conclusion PRF can release the cytokines constantly, taking the yunnan baiyao can stimulate the PRF to release higher concentration of cytokines effectively.
- Yunnan baiyao /
- Platelet rich-fibrin /
- Cytokines

HTML全文

参考文献(17)

[1]	[1]CHOUKROUN J, DISS A, SIMONPIERI A, et al.Platelet-rich fibrin (PRF) :A second generation platelet concentrate. Part V:Histologic evaluations of Choukroun’s PRF effects on bone allograft maturation in sinus lift[J].Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2006, 101 (3) :299-303.
[2]	[2] KANAYAMA T, HORII K, SENGA Y, et al. Crestal approach to sinus floor elevation for atrophic maxilla using platelet-rich fibrin as the only grafting material:a 1-year prospective study[J].Implant Dent, 2016, 25 (1) :32-38.
[3]	[3]MOUSSA M, EL-DAHAB OA, EL NAHASS H. Anterior maxilla augmentation using palatal bone block with platelet rich fibrin:a controlled tria[J].Int J Oral Maxillofac Implants, 2016, 31 (3) :708-715.
[4]	[4] DAS S, JHINGRAN R, BAINS VK, et al. Socket preservation by beta-tri-calcium phosphate with collagen compared to platelet-rich fibrin:a clinic-radiographic study[J].Eur J Dent, 2016, 10 (2) :264-276.
[5]	[5]KANAYAMA T, HORII K, SENGA Y, et al. Crestal approach to sinus floor elevation for atrophic maxilla using platelet-rich fibrin as the only grafting material:a 1-year prospective study[J].Implant Dent, 2016, 25 (1) :32-38.
[6]	[6]MOUSSA M, EL-DAHAB OA, EL NAHASS H. Anterior maxilla augmentation using palatal bone block with platelet rich fibrin:a controlled trial[J].Oral Maxillofac Implants, 2016, 31 (3) :708-715.
[7]	[7]WAGNER W, WEHRMANN M.Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin[J].Cell Mol Med, 2007, 11 (6) :1342-1351.
[8]	[8]HAUSER F, GAYDAROV N, BADOUD L, et al. Clinical and histologyical evaluation of postextraction platelet-rich fibrin socket filling:a prospective randomized controlled study[J].Implant Dent, 2013, 22 (3) :295-303.
[9]	[9]DAS S, JHINGRAN R, BAINS VK, et al. Socket preservation by betatricalcium phosphate with collagen compared to platelet-rich fibrin:a clinic-radiographic study[J].Dent, 2016, 10 (2) :264-276.
[10]	[10]MARRELLI M, TATULLO M. Influence of PRF in the healing of bone and gingival tissues. Clinical and histological evaluations[J].Eur Rev Med Pharmacol Sci, 2013, 17 (14) :1958-1962.
[11]孙洁, 张剑明, 李彦秋, 富血小板纤维蛋白超微结构的观察与探讨[J].口腔医学研究, 2010, 26 (01) :98-101.
[12]张小梅, 莫治强, 李俊明, 等.云南白药对围手术期血小板表面糖蛋白及超微结构影响的研究[J].昆明医学院学报, 2004, 25 (2) :56-59.
[13]杨吉林, 莫志强, 罗用宇, 等.口服云南白药胶囊对骨科病人术中出血量影响的研究[J].云南医药, 2003, 46 (3) :175-178.
[14]唐正龙, 王兴, 伊彪, 等.云南白药对血小板的释放与代谢反应的影响[J].贵阳医学院学报, 2009, 34 (3) :282-285.
[15]	[15]GIANNINI S, CIELO A, BONANOME L, et al.Comparison between PRP, PRGF and PRF:Lights and shadows in three similar but different protocols[J].Europcan Review for Medical and Pharmacological Sciences, 2015, 19 (6) :927-930.
[16]	[16]DOHAN DM, CHOUKROUN J, DISS A, et al.Platelet-rich fibrin (PRF) :a second-generation platelet concentrate. Part II:platelet-related biologic features[J].Oral Surg Oral Med Oral Radiol Endod, 2006, 101 (3) :e51-55.
[17]白彭, 叶平.两种胶原膜暴露于El腔环境中降解作用的对照研究[J].中国口腔种植学杂志, 2011, 16 (1) :56-60.

相关文章(20)

[1]	袁艳波, 阮兆娟, 曹良菊, 杨万娟, 蒋愉, 周雪. 2型糖尿病患者浓缩生长因子纤维蛋白凝胶含量分析, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230720
[2]	周箐竹, 龚桌, 赵娴, 刘博言, 黄文莉, 赫佳, 刘旒, 浦仆飞. 重组人表皮生长因子对云南高海拔地区敏感性肌肤的修复效果, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220627
[3]	蒋玉娥, 钟兆铭, 高艳章, 王伟, 孙瑞梅, 孙传政. 转化生长因子β1在血小板促进甲状腺未分化癌侵袭迁移中的作用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20221201
[4]	廖勇, 郭一雲, 周红宇, 冯海魄, 陈根定, 杨水清. 叶酸联合阿伦磷酸钠对绝经后骨质疏松患者骨代谢、CCL4及纤维细胞生长因子水平的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210716
[5]	金锦花, 李科岩, 张玲, 王玉慧, 邓晓明. 成纤维细胞生长因子受体FGFR1真核表达质粒的构建及其在HEK293T细胞的表达, 昆明医科大学学报.
[6]	刘俊, 和红兵, 任晓斌. 云南白药对LPS诱导小鼠头颅骨炎症模型NF-κ B和NFATc1的影响, 昆明医科大学学报.
[7]	谭锦章, 和红兵, 任晓斌, 王礼钰. 云南白药联合辅酶Q10辅助治疗慢性牙周炎临床疗效, 昆明医科大学学报.
[8]	衡旭华, 包晓红, 邹剑杰, 李长龙, 唐洁, 刘明春, 倪焕娟. 高血压患者血清血小板衍生生长因子-B与颈动脉IMT的相关性, 昆明医科大学学报.
[9]	储雯. 云南白药对体外培养人牙周膜成纤维细胞C-fos、C-jun基因表达的影响, 昆明医科大学学报.
[10]	赵瑜. 人血管内皮生长因子A（VEGFA）的原核载体构建、蛋白表达及鉴定, 昆明医科大学学报.
[11]	李璠. 单核细胞趋化蛋白-1和血管内皮生长因子mRNA在急性甲醇中毒大鼠脑组织的表达, 昆明医科大学学报.
[12]	刘栋. 血清生存素水平及结缔组织生长因子对慢性乙型肝炎纤维化进展的临床意义, 昆明医科大学学报.
[13]	张敏. 外源性碱性成纤维细胞生长因子诱导下自体静脉桥接修复面神经缺损, 昆明医科大学学报.
[14]	蒋爱梅. 脂肪干细胞结合碱性成纤维细胞生长因子辅助颗粒脂肪移植的实验研究, 昆明医科大学学报.
[15]	张敏. 翻转静脉桥接加碱性成纤维细胞生长因子修复面神经缺损的实验研究, 昆明医科大学学报.
[16]	. 云南白药对骨折愈合过程中血管内皮生长因子表达的影响, 昆明医科大学学报.
[17]	. 氟康唑、云南白药、铝镁加混悬液联合治疗霉菌性食管炎52例分析, 昆明医科大学学报.
[18]	刀保细. 氟康唑、云南白药、铝镁加混悬液联合治疗霉菌性食管炎52例分析, 昆明医科大学学报.
[19]	. 云南白药在鼻内窥镜术围手术期止血的疗效观察, 昆明医科大学学报.
[20]	杨晓文. 高迁移率族蛋白1及生长因子受体Ⅲ在乳腺癌组织中的表达及其临床意义, 昆明医科大学学报.