G6PD表达敲减对人宫颈癌细胞miRNAs表达谱的影响

胡滔; 刘光彩; 洪颖; 陈红兰; 常业飞; 姜水; 檀雅欣; 习杨彦彬; 陈波

G6PD表达敲减对人宫颈癌细胞miRNAs表达谱的影响

1. 云南省第三人民医院医学检验科
2. 昆明医科大学基础医学院
3. 昆明医科大学科研实验中心

基金项目:

基金：国家自然科学基金资助项目 (81502377); 云南省应用基础研究计划资助项目 (2016FB139); 云南省教育厅科学研究基金资助项目 (2016ZDX044);

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出版历程
- 收稿日期: 2018-07-14

Effect of G6PD Knockdown on the Expression of miRNAs in Cervical Cancer Cells

Funds:

基金：国家自然科学基金资助项目 (81502377); 云南省应用基础研究计划资助项目 (2016FB139); 云南省教育厅科学研究基金资助项目 (2016ZDX044);

摘要

摘要: 目的比较G6PD表达敲减后人宫颈癌细胞HPV-C33A与HPV16+Siha以及HPV18+Hela中mi RNAs的表达差异, 进一步了解G6PD致瘤的分子机制。方法构建G6PD表达敲减的人宫颈癌细胞株, 运用mi RNAs芯片对各细胞株中mi RNAs进行对比分析, q RT-PCR对结果进行验证。结果 (1) 成功获得G6PD表达敲减的人宫颈癌细胞株C33A、Siha和Hela; (2) 与HPV-C33A相比, G6PD表达干扰后高危型HPV16+/18+人宫颈癌细胞株中出现多种mi RNAs差异表达的情况 (P<0.05) 。结论 G6PD在高危型HPV+人宫颈癌中的致瘤性与多种mi RNAs的差异表达相关。
- 宫颈癌 /
- 高危型人乳头瘤病毒16/18型 /
- G6PD /
- miRNAs
Abstract: Objective To explore the expression changes of miRNAs in HRHPV (16 and 18) infected (+) cervical cancer cells after G6 PD expression knock-down. Methods The G6 PD expression in HPV-C33 A, HPV16+Siha and HPV18+Hela cells was inhibited by RNA interference target G6 PD. Human micro RNA Microarray Kit and q RT-PCR were employed to determine the expression changes of mi RNA between different cell lines.Results The expression of G6 PD significantly decreased after RNA interference in different cervical cancer cell lines.Mi RNA microarrayand q RT-PCR analysis revealed that numerousmiRNAs in HPV+ Hela/Siha cells were expressional different from the control HPV-C33 A cells, following G6 PD knockdown. Conclusion The expression changes of various miRNAs following G6 PDknockdown are associated with the oncogenesis events of G6 PD in HR HPV infected cervical cancer cells.
- Cervical cancer /
- High-risk human papillomavirus (HR HPV) 16/18 infection /
- G6PD /
- miRNAs

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参考文献(24)

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