根尖牙乳头干细胞成骨分化的研究进展

钱石兵; 史会萍; 李艳秋; 杨镕羽; 段开文

根尖牙乳头干细胞成骨分化的研究进展

1.
曲靖医学高等专科学校口腔学院，云南曲靖　655100
2.
昆明市延安医院口腔科，云南昆明　650051
3.
大理大学口腔学院，云南大理　671003

基金项目: 国家自然科学基金资助项目（81160135）；云南省生物医药重大科技专项基金（202102AA100007）；云南省教育厅科学研究基金教师类项目（2024J1609）

详细信息

作者简介:
钱石兵（1996～），男，云南曲靖人，医学硕士，助教，主要从事牙周病和口腔黏膜疾病的防治工作

通讯作者:
段开文，E-mail：kwduan@aliyun.com

中图分类号: R78
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- HTML全文浏览量: 83
- PDF下载量: 2
- 被引次数: 0
出版历程
- 收稿日期: 2023-12-05
- 网络出版日期: 2024-09-03

Research Progress on Osteogenic Differentiation of Apical Papilla Stem Cells

1.
School of Stomatology，Qujing Medical College，Qujing Yunnan 655100
2.
Dept. of Stomatology，The Affiliated Yan’an Hospital，Kunming Yunnan 650051
3.
School of Stomatology，Dali University，Dali Yunnan 671003，China

摘要

摘要: 根尖牙乳头干细胞（stem cells from apical papilla，SCAP）具有很强的多系分化潜能，其中成骨分化可以应用于骨组织再生，为口腔颌骨缺损治疗提供新思路。成骨分化是个复杂的网络调控过程，诸如各种细胞因子、表观遗传物质、各种信号分子和信号通路等内源性物质均可产生不同程度的影响。这些因素相互作用可以促进SCAP的增殖、迁移和成骨分化，但其在SCAP成骨分化的不同进程中的具体机制和内在联系各不相同。对近年来有关促进SCAP成骨分化的各种因素及其可能的调控机制研究文献进行综述，以期为其进一步的应用研究提供新信息。
- 根尖牙乳头干细胞 /
- 成骨分化 /
- 细胞因子 /
- 表观遗传 /
- 信号通路
Abstract: Stem cells from apical papilla （SCAP） have a strong multi-line differentiation potential, in which osteogenic differentiation can be applied to bone tissue regeneration, providing a new idea for the treatment of oral jaw defects. Osteogenic differentiation is a complex network regulation process, and endogenous substances such as various cytokines, epigenetic material, various signaling molecules and signaling pathways can have different degrees of influence. The interaction of these factors can promote the proliferation, migration and osteogenic differentiation of SCAP, but the specific mechanisms and internal links in different processes of osteogenic differentiation of SCAP are different. In this paper, the factors that promote osteogenic differentiation of SCAP and their possible regulatory mechanisms were reviewed to provide new information for further application research.
- Apical papilla stem cells /
- Osteogenic differentiation /
- Cytokines /
- Epigenetic inheritance /
- Signal path

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参考文献(50)

[1]	Morsczeck C. Dental stem cells for tooth regeneration: how far have we come and where next?[J]. Expert Opin Biol Ther,2023,23(6):527-537. doi: 10.1080/14712598.2023.2208268
[2]	Sonoyama W,Liu Y,Fang D,et al. Mesenchymal stem cell-mediated functional tooth regeneration in swine[J]. PLoS One,2006,1(1):e79-86. doi: 10.1371/journal.pone.0000079
[3]	Liu Q,Gao Y,He J. Stem cells from the apical papilla (SCAPs): past,present,prospects,and challenges[J]. Biomedicines,2023,11(7):2047-2059. doi: 10.3390/biomedicines11072047
[4]	Bakopoulou A,Leyhausen G,Volk J,et al. Comparative analysis of in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP)[J]. Arch Oral Biol,2011,56(7):709-721. doi: 10.1016/j.archoralbio.2010.12.008
[5]	Chen K,Xiong H,Huang Y,et al. Comparative analysis of in vitro periodontal characteristics of stem cells from apical papilla (SCAP) and periodontal ligament stem cells (PDLSCs)[J]. Arch Oral Biol,2013,58(8):997-1006. doi: 10.1016/j.archoralbio.2013.02.010
[6]	Chang H H,Chang M C,Wu I H,et al. Role of ALK5/Smad2/3 and MEK1/ERK signaling in transforming growth factor beta 1-modulated growth,collagen turnover,and differentiation of stem cells from apical papilla of human tooth[J]. J Endod,2015,41(8):1272-1280. doi: 10.1016/j.joen.2015.03.022
[7]	Li J,Ge L,Zhao Y,et al. TGF-beta2 and TGF-beta1 differentially regulate the odontogenic and osteogenic differentiation of mesenchymal stem cells[J]. Arch Oral Biol,2022,64(3):105357.
[8]	Yu S,Li J,Zhao Y,et al. Comparative secretome analysis of mesenchymal stem cells from dental apical papilla and bone marrow during early odonto/osteogenic differentiation: potential role of transforming growth factor-beta2[J]. Front Physiol,2020,11(3):41-53.
[9]	Zhang W,Zhang X,Ling J,et al. Proliferation and odontogenic differentiation of BMP2 genetransfected stem cells from human tooth apical papilla: an in vitro study[J]. Int J Mol Med,2014,34(4):1004-1012. doi: 10.3892/ijmm.2014.1862
[10]	Zhang W,Zhang X,Li J,et al. Foxc2 and BMP2 induce osteogenic/odontogenic differentiation and mineralization of human stem cells from apical papilla[J]. Stem Cells Int,2018,9(7):2363917.
[11]	Zhang W,Zhang X,Ling J,et al. Osteo-/odontogenic differentiation of BMP2 and VEGF gene-co-transfected human stem cells from apical papilla[J]. Mol Med Rep,2016,13(5):3747-3754. doi: 10.3892/mmr.2016.4993
[12]	Press T,Viale-Bouroncle S,Felthaus O,et al. EGR1 supports the osteogenic differentiation of dental stem cells[J]. Int Endod J,2015,48(2):185-192. doi: 10.1111/iej.12299
[13]	Wang J,Zhang H,Zhang W,et al. Bone morphogenetic protein-9 effectively induces osteo/odontoblastic differentiation of the reversibly immortalized stem cells of dental apical papilla[J]. Stem Cells Dev,2014,23(12):1405-1416. doi: 10.1089/scd.2013.0580
[14]	Wang F,Jiang Y,Huang X,et al. Pro-Inflammatory cytokine TNF-alpha attenuates BMP9-induced osteo/ odontoblastic differentiation of the stem cells of dental apical papilla (SCAPs)[J]. Cell Physiol Biochem,2017,41(5):1725-1735. doi: 10.1159/000471865
[15]	Zhang H,Wang J,Deng F,et al. Canonical Wnt signaling acts synergistically on BMP9-induced osteo/odontoblastic differentiation of stem cells of dental apical papilla (SCAPs)[J]. Biomaterials,2015,39(1):145-154.
[16]	Zhu X Y,Diao S,Yang D M,et al. The Mechanism of GREM1's effect on osteogenic/odontogenic differentiation of stem cells from apical papilla[J]. Sichuan Da Xue Xue Bao Yi Xue Ban,2021,52(3):409-415.
[17]	Wang S,Mu J,Fan Z,et al. Insulin-like growth factor 1 can promote the osteogenic differentiation and osteogenesis of stem cells from apical papilla[J]. Stem Cell Res,2012,8(3):346-356. doi: 10.1016/j.scr.2011.12.005
[18]	Wang Y,Pang X,Wu J,et al. MicroRNA hsa-let-7b suppresses the odonto/osteogenic differentiation capacity of stem cells from apical papilla by targeting MMP1[J]. J Cell Biochem,2018,119(8):6545-6554. doi: 10.1002/jcb.26737
[19]	Ma S,Liu G,Jin L,et al. IGF-1/IGF-1R/hsa-let-7c axis regulates the committed differentiation of stem cells from apical papilla[J]. Sci Rep,2016,6(11):36922-36933.
[20]	Cao Y,Xia D S,Qi S R,et al. Epiregulin can promote proliferation of stem cells from the dental apical papilla via MEK/Erk and JNK signalling pathways[J]. Cell Prolif,2013,46(4):447-456. doi: 10.1111/cpr.12039
[21]	Li Y,Yan M,Wang Z,et al. 17beta-estradiol promotes the odonto/osteogenic differentiation of stem cells from apical papilla via mitogen-activated protein kinase pathway[J]. Stem Cell Res Ther,2014,5(6):125-149. doi: 10.1186/scrt515
[22]	Wang Y,Lu Y,Li Z,et al. Oestrogen receptor alpha regulates the odonto/osteogenic differentiation of stem cells from apical papilla via ERK and JNK MAPK pathways[J]. Cell Prolif,2018,51(6):e12485-12494. doi: 10.1111/cpr.12485
[23]	Pang X,Zhuang Y,Li Z,et al. Intermittent administration of parathyroid hormone enhances odonto/osteogenic differentiation of stem cells from the apical papilla via JNK and P38 MAPK pathways[J]. Stem Cells Int,2020,11(2):5128128.
[24]	Zhang J,Zhao I S,Yu O Y,et al. Layer-by-layer self-assembly polyelectrolytes loaded with cyclic adenosine monophosphate enhances the osteo/odontogenic differentiation of stem cells from apical papilla[J]. J Biomed Mater Res A,2021,109(2):207-218. doi: 10.1002/jbm.a.37017
[25]	Su S,Zhu Y,Li S,et al. The transcription factor cyclic adenosine 3',5'-monophosphate response element-binding protein enhances the odonto/osteogenic differentiation of stem cells from the apical papilla[J]. Int Endod J,2017,50(9):885-894. doi: 10.1111/iej.12709
[26]	Zhang Y,Yuan L,Meng L,et al. Guanine and nucleotide binding protein 3 promotes odonto/osteogenic differentiation of apical papilla stem cells via JNK and ERK signaling pathways[J]. Int J Mol Med,2019,43(1):382-392.
[27]	Xiao M,Yao B,Zhang B D,et al. Stromal-derived Factor-1alpha signaling is involved in bone morphogenetic protein-2-induced odontogenic differentiation of stem cells from apical papilla via the Smad and Erk signaling pathways[J]. Exp Cell Res,2019,381(1):39-49. doi: 10.1016/j.yexcr.2019.04.036
[28]	Liu J,Wang X,Song M,et al. MiR-497-5p regulates osteo/odontogenic differentiation of stem cells from apical papilla via the smad signaling pathway by targeting smurf2[J]. Front Genet,2020,11(10):582366.
[29]	Li Z,Ge X,Lu J,et al. MiR-141-3p regulates proliferation and senescence of stem cells from apical papilla by targeting YAP[J]. Exp Cell Res,2019,383(2):111562. doi: 10.1016/j.yexcr.2019.111562
[30]	Xiao Y,Chen L,Xu Y,et al. Circ-ZNF236 mediates stem cells from apical papilla differentiation by regulating LGR4-induced autophagy[J]. Int Endod J,2024,57(4):431-450. doi: 10.1111/iej.14021
[31]	Jia Q,Chen X,Jiang W,et al. The regulatory effects of long noncoding RNA-ANCR on dental tissue-derived stem cells[J]. Stem Cells Int,2016,7(8):3146805.
[32]	Wang L,Yang H,Lin X,et al. KDM1A regulated the osteo/dentinogenic differentiation process of the stem cells of the apical papilla via binding with PLOD2[J]. Cell Prolif,2018,51(4):e12459-12467. doi: 10.1111/cpr.12459
[33]	Diao S,Yang D M,Dong R,et al. Enriched trimethylation of lysine 4 of histone H3 of WDR63 enhanced osteogenic differentiation potentials of stem cells from apical papilla[J]. J Endod,2015,41(2):205-211. doi: 10.1016/j.joen.2014.09.027
[34]	Gao R,Dong R,Du J,et al. Depletion of histone demethylase KDM2A inhibited cell proliferation of stem cells from apical papilla by de-repression of p15INK4B and p27Kip1[J]. Mol Cell Biochem,2013,379(1-2):115-122. doi: 10.1007/s11010-013-1633-7
[35]	Su X,Yang H,Shi R,et al. Depletion of SNRNP200 inhibits the osteo-/dentinogenic differentiation and cell proliferation potential of stem cells from the apical papilla[J]. BMC Dev Biol,2020,20(1):22-32. doi: 10.1186/s12861-020-00228-y
[36]	Xu J,Yu B,Hong C,et al. KDM6B epigenetically regulates odontogenic differentiation of dental mesenchymal stem cells[J]. Int J Oral Sci,2013,5(4):200-205. doi: 10.1038/ijos.2013.77
[37]	Li W,Lin X,Yang H,et al. Depletion of HOXA5 inhibits the osteogenic differentiation and proliferation potential of stem cells from the apical papilla[J]. Cell Biol Int,2018,42(1):45-52. doi: 10.1002/cbin.10860
[38]	Gao R T,Zhan L P,Meng C,et al. Homeobox B7 promotes the osteogenic differentiation potential of mesenchymal stem cells by activating RUNX2 and transcript of BSP[J]. Int J Clin Exp Med,2015,8(7):10459-10470.
[39]	Yang H,Liang Y,Cao Y,et al. Homeobox C8 inhibited the osteo-/dentinogenic differentiation and migration ability of stem cells of the apical papilla via activating KDM1A[J]. J Cell Physiol,2020,235(11):8432-8445. doi: 10.1002/jcp.29687
[40]	Wu Z,Wang J,Dong R,et al. Depletion of MEIS2 inhibits osteogenic differentiation potential of human dental stem cells[J]. Int J Clin Exp Med,2015,8(5):7220-7230.
[41]	Yang H,Fan J,Cao Y,et al. Distal-less homeobox 5 promotes the osteo-/dentinogenic differentiation potential of stem cells from apical papilla by activating histone demethylase KDM4B through a positive feedback mechanism[J]. Exp Cell Res,2019,374(1):221-230. doi: 10.1016/j.yexcr.2018.11.027
[42]	Yang H,Cao Y,Zhang J,et al. DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013[J]. Stem Cell Res Ther,2020,11(1):271-286. doi: 10.1186/s13287-020-01791-8
[43]	Wan F,Gao L,Lu Y,et al. Proliferation and osteo/odontogenic differentiation of stem cells from apical papilla regulated by Zinc fingers and homeoboxes 2: An in vitro study[J]. Biochem Biophys Res Commun,2016,469(3):599-605. doi: 10.1016/j.bbrc.2015.11.135
[44]	Zhang J,Wang Z,Jiang Y,et al. Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro[J]. Exp Cell Res,2015,332(2):259-266. doi: 10.1016/j.yexcr.2015.01.020
[45]	Wang H,Cao Y. WIF1 enhanced dentinogenic differentiation in stem cells from apical papilla[J]. BMC Oral Health,2019,19(1):25-32. doi: 10.1186/s12903-018-0700-6
[46]	Jin L,Cao Y,Yu G,et al. SFRP2 enhances the osteogenic differentiation of apical papilla stem cells by antagonizing the canonical WNT pathway[J]. Cell Mol Biol Lett,2017,22(8):14-27.
[47]	Yang H,Li G,Han N,et al. Secreted frizzled-related protein 2 promotes the osteo/odontogenic differentiation and paracrine potentials of stem cells from apical papilla under inflammation and hypoxia conditions[J]. Cell Prolif,2020,53(1):e12694-12704. doi: 10.1111/cpr.12694
[48]	Zhou M,Guo S,Yuan L,et al. Blockade of LGR4 inhibits proliferation and odonto/osteogenic differentiation of stem cells from apical papillae[J]. J Mol Histol,2017,48(5-6):389-401. doi: 10.1007/s10735-017-9737-0
[49]	Cheng Q,Zeng K,Kang Q,et al. The antimicrobial peptide LL-37 promotes migration and odonto/osteogenic differentiation of stem cells from the apical papilla through the Akt/Wnt/beta-catenin signaling pathway[J]. J Endod,2020,46(7):964-972. doi: 10.1016/j.joen.2020.03.013
[50]	Liu J,Du J,Chen X,et al. The effects of mitogen-activated protein kinase signaling pathways on lipopolysaccharide-mediated osteo/odontogenic differentiation of stem cells from the apical papilla[J]. J Endod,2019,45(2):161-167. doi: 10.1016/j.joen.2018.10.009

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