Establishment of a Smooth Muscle Cell Model for Venous Grafts After Coronary Bypass Surgery
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摘要:
目的 探索冠脉搭桥静脉桥体外细胞模型的建立。 方法 体外培养大鼠静脉平滑肌细胞，分4组，细胞拉伸装置不同拉伸幅度拉伸细胞，通过观察细胞形态变化，CCK8检测观察细胞活性变化，Edu染色观察细胞增殖变化，划痕实验观察细胞迁移能力变化。 结果 与正常对照组相比较，5%张应力对细胞活度无显著影响（P > 0.05）；10%张应力明显促进细胞活力（P < 0.05）；而 15%张应力则明显抑制细胞活力（P < 0.01）。 与正常对照组相比，5%张应力对细胞增殖无显著影响（P > 0.05）；10%张应力明显促进细胞增殖（P < 0.01）；15%张应力明显抑制细胞增殖（P < 0.05）。与正常组对比，5%张应力对细胞迁移能力无明显影响（P > 0.05）；10%张应力促进细胞迁移能力（P < 0.05）；15%张应力明显抑制细胞迁移能力（P < 0.05）。 结论 在频率1HZ，拉伸变形幅度10%参数设定下，血管平滑肌细胞与临床静脉桥再狭窄增生细胞生物行为表现一致。 Abstract:Objective To explore the establishment of in vitro model of coronary artery bypass venous bridge in coronary artery bypass graft （CABG）. Methods Rat venous smooth muscle cells were cultured in vitro and divided into 4 groups. The cells were stretched by cell stretching device in order to simulate the mechanical environment of vein bridge. The changes of cell morphology were observed by CCK8 detection, cell activity was observed by Edu staining, and cell migration ability was observed by scratch test. Results Compared to the normal control group, 5% tensile stress had no significant effect on cell viability （P > 0.05）; 10% tensile stress significantly enhanced cell vitality （P < 0.05）; while 15% tensile stress significantly suppressed cell vitality （P < 0.01）. In terms of cell proliferation, 5% tensile stress showed no significant effect compared to the normal control group （P > 0.05）; 10% tensile stress significantly promoted cell proliferation （P < 0.01）; and 15% tensile stress significantly inhibited cell proliferation （P < 0.05）. Compared to the normal group, 5% tensile stress had no obvious impact on cell migration capability （P > 0.05）; 10% tensile stress promoted cell migration ability （P < 0.05）; and 15% tensile stress significantly inhibited cell migration ability （P < 0.05）. Conclusion Under the parameters of 1 Hz frequency and 10% stretch deformation, the biological behavior of vascular smooth muscle cells is consistent with that of clinical stenosis-restenosis cells. -
Key words:
- venous bridge /
- model /
- in vitro /
- venous smooth muscle cells /
- coronary artery bypass graft
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图 1 各组VSMC形态观察（×25）
A：正常对照组；B：5%张应力组；C：10% 张应力组；D：15%张应力组。
Figure 1. Morphology in each group of VSMC
图 3 各组细胞活力统计分析结果
与正常对照组比较，ns P > 0.05，**P > 0.05，***P > 0.01。
Figure 3. Statistical analysis of CCK8 in each group of VSMC
图 4 各组EDU染色结果
与正常对照组比较，**P < 0.05，***P < 0.01。
Figure 4. EDU staining in each group of VSMC
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