Effect of Osteoking on Bone Density and Bone Biomechanics of Tree Shrews in a Postmenopausal Osteoporotic Fracture Model
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摘要:
目的 研究恒古骨伤愈合剂对绝经后骨质疏松性骨折（OPF）模型树鼩骨密度及骨生物力学的影响。 方法 雌性滇西亚种树鼩双侧卵巢及子宫切除术建立骨质疏松（OP）模型，180 d后，对树鼩进行骨密度（BMD）测定，将20只OP模型造模成功的树鼩随机平均分为给药组（3 mL/kg）、模型组（等体积0.9%氯化钠溶液）。10只仅切除同体积大网膜树鼩分为正常组（等体积0.9%氯化钠溶液）。将给药组和模型组树鼩进行右侧后肢胫骨骨折术建立OPF模型。给药组每2天给药1次，连续给药90 d，其余组给予同剂量的生理盐水。处死树鼩后，进行双能X射线吸收法检测实验以及股骨三点弯曲实验。 结果 与正常组比较，给药组和模型组全身（含头部）、腰 + 盆骨、颈椎-尾椎、左骨盆、右骨盆、腰椎、腰 + 骶骨、全身（不含头部）BMD均有不同程度显著降低，差异有统计学意义（P < 0.05）；与模型组比较，给药组全身（含头部）、腰 + 盆骨、颈椎-尾椎、左骨盆、腰椎、腰 + 骶骨BMD均有显著提升，差异有统计学意义（P < 0.05），而右骨盆、全身（不含头部）BMD无统计学差异（ P > 0.05）。骨生物力学方面，与正常组相比，给药组能量吸收能力显著提升，而最大载荷和结构刚度无统计学差异（ P > 0.05）；模型组最大载荷、结构刚度、能量吸收能力均有显著降低，差异均有统计学意义（P < 0.05）；与给药组相比，模型组最大载荷、结构刚度、能量吸收能力均有显著降低，差异均有统计学意义（P < 0.05）。 结论 恒古骨伤愈合剂对OPF模型树鼩的BMD和骨生物力学方面均有改善作用。 Abstract:Objective To observe the effect of osteoking on bone density and bone biomechanics in a postmenopausal osteoporotic fracture（OPF）model tree shrew. Methods Female western Yunnan subspecies tree shrews were subjected to bilateral ovariectomy and hysterectomy to establish an osteoporosis（OP）model, and after 180 days, bone density（BMD）was measured in tree shrews, and 20 OP modeling successes were randomly and equally divided into administration（3 mL/kg）and model groups（equal volume of 0.9% sodium chloride solution）. Ten tree shrews with the same volume of large omentum removed were divided into normal groups（equal volume of 0.9% sodium chloride solution）. The tree shrews in the drug-administered and model groups were subjected to right hindlimb tibial fracture surgery to establish the OPF model. The administration group was given one dose of saline every 2 d for 90 d, and the remaining group was given the same dose of saline. Results Compared with the normal group, the BMD of whole body（including head）, lumbar + pelvic, cervical-caudal, left pelvis, right pelvis, lumbar spine, lumbar + sacral, and whole body（excluding head）were significantly reduced to different degrees in the administered and model groups, and the differences were statistically significant（P < 0.05）. Compared with the model group, the BMD of the whole body（including the head）, lumbar + pelvic, cervical-caudal, left pelvis, lumbar spine, and lumbar + sacral were significantly increased in the administration group, and the differences were statistically significant（P < 0.05）, while the BMD of the right pelvis and whole body（excluding the head）were not statistically different. In terms of bone biomechanics, energy absorption was significantly increased in the administered group compared to the normal group, while maximum load and structural stiffness were not statistically difference; the maximum load, structural stiffness, and energy absorption capacity of the model group were all significantly reduced, and the differences were statistically significant（P < 0.05）. The maximum load, structural stiffness, and energy absorption capacity of the model group were all significantly reduced compared to the drug-administered group, and the differences were statistically significant（P < 0.05）. Conclusion Osteoking can improve both BMD and bone biomechanics in the OPF model of tree shrew. -
Key words:
- Osteoking /
- Tree shrew /
- Osteoporosis /
- Bone density /
- Bone biomechanics
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表 1 各组树鼩骨密度比较结果[（
$\bar x \pm s$ ），g/cm2]Table 1. Comparison of bone density of tree shrews in each group[（
$\bar x \pm s$ ），g/cm2]区域 正常组（n = 10） 给药组（ n = 10） 模型组（ n = 10） 全身（含头部） 0.103 ± 0.012 0.097 ± 0.005* 0.087 ± 0.005**.# 腰 + 盆骨 0.097 ± 0.004 0.088 ± 0.003** 0.083 ± 0.004**.# 颈椎−尾椎 0.096 ± 0.003 0.084 ± 0.004** 0.078 ± 0.005**.## 左骨盆 0.099 ± 0.011 0.087 ± 0.006** 0.082 ± 0.004**.# 右骨盆 0.098 ± 0.005 0.091 ± 0.004* 0.088 ± 0.005** 腰椎 0.096 ± 0.002 0.090 ± 0.004** 0.083 ± 0.005**.# 腰 + 骶骨 0.093 ± 0.006 0.087 ± 0.004* 0.081 ± 0.005**.# 全身（不含头部） 0.092 ± 0.003 0.084 ± 0.004* 0.082 ± 0.004** 与正常组比较，*P < 0.05，**P < 0.01；与给药组比较，#P < 0.05，##P < 0.01。 
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表 2 各组树鼩骨生物力学特性统计分析结果[（
$\bar x \pm s$ ），n = 10]Table 2. Results of statistical analysis of the biomechanical properties of tree shrew bone in each group[（
$\bar x \pm s$ ），n = 10]项目 正常组 给药组 模型组 最大载荷（N） 50.13 ± 7.75 47.75 ± 4.43 41.31 ± 4.43**.## 结构刚度（N/mm） 72.52 ± 19.76 76.63 ± 6.42 53.18 ± 7.99**.## 能量吸收能力（N/mm） 53.64 ± 12.21 59.95 ± 9.81* 41.52 ± 8.32**.## 与正常组比较，*P < 0.05，**P < 0.01；与给药组比较，##P < 0.01。
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