肠道菌群结构在非酒精性脂肪性肝病患者中的改变

薛平燕; 江艳; 徐玉善; 袁惠; 李璇; 宋亚贤; 刘华

doi:10.12259/j.issn.2095-610X.S20201120

肠道菌群结构在非酒精性脂肪性肝病患者中的改变

doi: 10.12259/j.issn.2095-610X.S20201120

薛平燕^{1, 2},
江艳¹,
徐玉善^1, ,,
袁惠³,
李璇⁴,
宋亚贤¹,
刘华⁵

1.
昆明医科大学第一附属医院内分泌一科，云南昆明　650032
2.
曲靖市第二人民医院内分泌科，云南曲靖　655000
3.
昆明医科大学第一附属医院超声医学科
4.
神经外二科
5.
检验科，云南昆明　650032

基金项目: 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2017FE468（-044）]

详细信息

作者简介:
薛平燕（1983～），女，云南宣威人，医学硕士，主治医师，主要从事糖尿病及其并发症研究工作

通讯作者:
徐玉善，E-mail：xuyushan1019@126.com

中图分类号: R575.4
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出版历程
- 收稿日期: 2020-09-21
- 网络出版日期: 2020-12-03
- 刊出日期: 2020-11-25

Changes of Intestinal Flora in Non-alcoholic Fatty Liver Disease

Ping-yan XUE^{1, 2},
Yan JIANG¹,
Yu-shan XU^{1
, ,},
Hui YUAN³,
Xuan LI⁴,
Ya-xian SONG¹,
Hua LIU⁵

1.
Dept. of Endocrinology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032
2.
Dept. of Endocrinology，The 2nd People’s Hospital of Qujing City，Qujing Yunnan 655000
3.
Dept. of Ultrasound Medicine
4.
Dept. of Neurosurgery
5.
The Medical Laboratory Department，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

摘要

摘要: 目的测定非酒精性脂肪性肝病（nonalcoholic fatty liver disease，NAFLD）患者肠道中目标细菌的数量，探讨肠道菌群在NAFLD发生发展中的作用。方法随机选取150例NAFLD患者，60例健康对照者，采用实时荧光定量PCR法检测粪便中目标细菌DNA的表达量。结果 NAFLD患者粪便中的乳酸杆菌、直肠真杆菌数量显著增加（P = 0.00、P = 0.007），双歧杆菌、多形拟杆菌数量显著降低（P = 0.00、P = 0.022）。结论 NAFLD患者肠道中存在乳酸杆菌、直肠真杆菌、双歧杆菌、多形拟杆菌的结构失衡，肠道菌群失衡可能参与NAFLD的发生发展，改善肠道菌群失衡对NAFLD具有一定治疗价值。
- 肠道菌群 /
- NAFLD /
- 拟杆菌门 /
- 厚壁菌门
Abstract: Objective To observe the changes of intestinal flora in patients with non-alcoholic fatty liver disease （NAFLD） and the role in occurrence and development of the disease. Methods One hundred and fifty patients who were diagnosed as NAFLD and 60 healthy controls were randomly selected. Used the relative quantification of Real Time PCR to detecte the expression level of DNA of target bacteria. Results Compared to the control group, the quantity of Lactobacillus and Eubacterium rectale in NAFLD patients were significant increased, Bifidobacterium and Bacteroides thetaiotaomicron were decreased. Conclusion The quantity of Lactobacillus, Eubacterium rectale, Bifidobacterium, Bacteroides thetaiotaomicron in patients with NAFLD may exist structural imbalance of intestinal flora, gut microbiome probably participate in the occurrence and development of NAFLD, improve the intestinal flora imbalance has a certain treatment value in NAFLD.
- Intestinal flora /
- Non-alcoholic fatty liver disease /
- Phylum firmicutes /
- Bacteroidetes

HTML全文

图 1 多形拟杆菌

Figure 1. Bacteroides thetaiotaomicron

下载: 全尺寸图片幻灯片

图 2 直肠真杆菌

Figure 2. Eubacterium rectale

下载: 全尺寸图片幻灯片

图 3 双歧杆菌

Figure 3. Bifidobacterium

下载: 全尺寸图片幻灯片

图 4 乳酸杆菌

Figure 4. Lactobacillus

下载: 全尺寸图片幻灯片

图 5 GAPDH

Figure 5. GAPDH

下载: 全尺寸图片幻灯片

图 6 溶解曲线图

A：多形拟杆菌；B：直肠真杆菌；C：双歧杆菌；D：乳酸杆菌；E：GAPDH。

Figure 6. Melted curve

下载: 全尺寸图片幻灯片

图 7 实时PCR扩增曲线图

A：多形拟杆菌；B：直肠真杆菌；C：双歧杆菌；D：乳酸杆菌；E：GAPDH。

Figure 7. The curve of real-time PCR

下载: 全尺寸图片幻灯片

表 1 NAFLD组与对照组目标细菌比较[M（P25，P75）]

Table 1. The target bacteria comparation of NAFLD and the control group[M（P25，P75）]

目标细菌	NAFLD组	正常对照组	Z	P
多形拟杆菌	1.99（0.671，6.311）	1.854，6.267	−2.23	0.022^*
直肠真杆菌	0.31（0.028，1.444）	0.003，0.340	−3.67	0.007^*
双歧杆菌	0.59（0.237，2.148）	1.053，10.602	−5.06	< 0.001^*
乳酸杆菌	0.24（0.001，0.247）	0.001，0.203	−2.68	< 0.001^*
^*P < 0.05。

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