右美托咪定抑制大鼠创伤性脑损伤后神经细胞凋亡

曹艳; 蒋鸿雁; 王艳雪; 吴抖威; 钱传云; 吴海鹰; 李坪

doi:10.12259/j.issn.2095-610X.S20210202

右美托咪定抑制大鼠创伤性脑损伤后神经细胞凋亡

doi: 10.12259/j.issn.2095-610X.S20210202

曹艳¹,
蒋鸿雁¹,
王艳雪²,
吴抖威¹,
钱传云³,
吴海鹰^3, ,,
李坪^1, ,

1.
昆明医科大学人体解剖学与组织胚胎学系，云南昆明　650500
2.
郑州大学附属郑州中心医院急诊科，河南郑州　450007
3.
昆明医科大学第一附属医院急诊科，云南昆明　650500

基金项目: 国家自然科学基金资助项目（82060241，81960817）；云南省创新团队基金资助项目（2019HC014）

详细信息

作者简介:
曹艳（1996～），女，河南巩义人，在读硕士研究生，主要从事脑损伤和神经胶质细胞研究。蒋鸿雁与曹艳对本文有同等贡献

通讯作者:
吴海鹰，E-mail：why0209@hotmail.com

李坪，E-mail：kyzplp@163.com

中图分类号: R651.15
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出版历程
- 收稿日期: 2020-12-09
- 刊出日期: 2021-03-05

Dexmedetomidine Inhibits Neuronal Apoptosis after Traumatic Brain Injury in Rats

1.
Dept. of Human Anatomy and Histology & Embryolog，Kunming Medical University，Kunming Yunnan 650500
2.
Dept. of Emergency，Zhengzhou Central Hospital Affiliated to Zhengzhou University，Henan Zhengzhou 450007
3.
Dept. of Emergency，The First Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

摘要

摘要: 目的探讨右美托咪定（DEX）对创伤性脑损伤（TBI）的神经保护作用是否与抑制神经细胞凋亡有关，是否涉及SIRT1信号通路。方法将SD大鼠随机分为Sham组、TBI + NS组、TBI + DEX组和TBI + DEX + EX527组。采用干/湿重法评估脑创伤后脑组织含水量；神经功能缺损评分检查神经功能受损情况；Western blot法检测大鼠皮质Bcl-2和Bax蛋白的表达水平。结果实验结果显示DEX干预后可减少脑创伤后促凋亡的调控蛋白Bax表达，差异有统计学意义（P < 0.05），增加抑制凋亡的调控蛋白Bcl-2表达，差异有统计学意义（P < 0.05），减轻脑水肿，差异有统计学意义（P < 0.05）和改善神经功能，差异有统计学意义（P < 0.05），上述观测指标的变化可被SIRT1抑制剂EX527逆转。结论提示DEX通过抑制神经细胞凋亡而减轻脑创伤后的继发性损害，该神经保护作用与SIRT1信号通路之间存在一定相关性。
- 右美托咪定 /
- 创伤性脑损伤 /
- 神经细胞凋亡
Abstract: Objective To discuss whether the neuroprotective effect of dexmedetomidine （DEX） against traumatic brain injury （TBI） is related to the inhibition of neuronal apoptosis and whether it is involved in SIRT1 signaling pathway. Methods SD rats were randomly divided into 4 groups: Sham group, TBI + NS group, TBI + DEX group and TBI + DEX + EX527 group. The water content of brain tissue after traumatic brain injury was evaluated by dry/wet weight method; the neurological function was evaluated by neurological severity score; the expression of Bcl-2 and Bax proteins in rat cortex were detected by Western blot. Results DEX decreased the expression of apoptosis-promoting regulatory protein Bax （P < 0.05）, increased the expression of apoptosis-inhibiting regulatory protein Bcl-2 （P < 0.05）, alleviated brain edema （P < 0.05） and improvedthe neurological function after traumatic brain injury （P < 0.05）. The changes of the above indexes were reversed by SIRT1 inhibitor EX527. Conclusions DEX can reduce the secondary damage after traumatic brain injury by inhibiting the apoptosis of nerve cells. There may be a certain correlation between the neuroprotective effect and SIRT1 signaling pathway.
- Dexmedetomidine /
- Traumatic brain injury /
- Neuronal apoptosis

HTML全文

图 1 DEX干预对TBI所致脑水肿的影响

与Sham组比较，*P < 0.05；与TBI + NS组比较，^#P < 0.05；与DEX50和DEX150组比较，^△P < 0.05。

Figure 1. Effect of DEX administration on TBI-induced brain edema

下载: 全尺寸图片幻灯片

图 2 DEX干预对TBI所致神经功能缺损的影响

与Sham组比较，*P < 0.05；与TBI + NS组比较，^#P < 0.05；与TBI + DEX100组比较，^△P < 0.05。

Figure 2. Effect of DEX administration on TBI-induced neurological deficits

下载: 全尺寸图片幻灯片

图 3 DEX干预对TBI后Bax和Bcl-2蛋白表达的影响

A：大脑皮层Bax和Bcl-2蛋白条带；B：Bax蛋白条带灰度值分析；C：Bcl-2蛋白条带灰度值分析。与Sham组比较，*P < 0.05；与TBI + NS组比较，^#P < 0.05；与TBI + DEX100组比较，^△P < 0.05。

Figure 3. Effect of DEX administration on Bax and Bcl-2 protein expression after TBI

下载: 全尺寸图片幻灯片

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