Influence of DC-Chol Cationic Liposome Adjuvant on the Immune Effect of Tetravalent Influenza Vaccine
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摘要:
目的 观察阳离子脂质体DC-Chol修饰的四价流感疫苗的免疫原性。 方法 采用薄膜分散结合冻融-冻干法制备DC-Chol脂质体,检测其包封率。将小鼠分为七组,为DC-Chol脂质体疫苗组(250 µg/只、500 µg/只、750 µg/只、900 µg/只)、PBS组、中性脂质体组、疫苗原液组(n = 3),通过MTT法确定DC-Chol脂质体的最佳剂量。将小鼠分为四组,DC-Chol脂质体组、疫苗原液组、中性脂质体组以及PBS对照组,腹腔免疫小鼠,于第7天、14天、28天处死,MTT法和T淋巴细胞表面标记实验检测小鼠淋巴细胞增殖情况观察免疫原性。 结果 MTT实验表明,DC-Chol修饰脂质体的最佳用量为500 µg/鼠(P < 0.05);T淋巴细胞表面标记实验显示,DC-Chol 阳离子脂质体能明显增强脾淋巴细胞数(P < 0.05)。 结论 与中性脂质体相比,DC-Chol 阳离子脂质体作为四价流感疫苗的佐剂具有良好的免疫增强作用。 Abstract:Objective To study the immunogenicity of the tetravalent influenza vaccine modified by cationic liposome DC-Chol. Methods DC-Chol liposomes were prepared by thin film dispersion-freeze-thaw- freeze-drying method, and the encapsulation rate was determined. The mice were divided into seven groups, DC-Chol liposome vaccine group (250 µg/mouse, 500 µg/mouse, 750 µg/mouse, 900 µg/mouse), PBS group, vaccine stock solution group, neutral liposome group (n = 3)Determine the optimal dose of DC-Chol liposomes by MTT method. The mice were divided into 4 groups, DC-Chol liposome group, vaccine stock solution group, neutral liposome group and PBS control group. Mice were immunized through the peritoneum and sacrificed on the 7, 14, and 28 days. The proliferation of mouse lymphocytes was detected by MTT method and T lymphocyte surface labeling experiment to study its immunogenicity. Results The MTT experiment showed that the optimal dose of DC-Chol modified liposomes was 500 μg/rat (P < 0.05); T lymphocyte surface labeling experiments showed that DC-Chol cationic liposomes significantly increased the number of splenic lymphocytes (P < 0.05). Conclusion Compared with neutral liposomes, DC-Chol cationic liposomes as an adjuvant of tetrovalent influenza vaccine has good immune enhancement effect. -
Key words:
- Liposome /
- DC-Chol /
- Influenza vaccine /
- Cellular immunity
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表 1 不同含量的DC-Chol阳离子脂质体的包封率
Table 1. Encapsulation efficiency of DC-Chol cationic liposomes with different contents
DC-Chol含量(µg/鼠) 包封率(%) 250 59.17 500 70.44 750 68.78 900 68.78 -
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