DC-Chol阳离子脂质体佐剂对流感疫苗免疫效果的影响

刘艳红; 赵祥月; 谢青昕; 普梦笛; 鲁卫东

doi:10.12259/j.issn.2095-610X.S20210302

DC-Chol阳离子脂质体佐剂对流感疫苗免疫效果的影响

doi: 10.12259/j.issn.2095-610X.S20210302

昆明医科大学药学院暨云南省天然药物药理重点实验室，云南昆明　650500

基金项目: 云南省生物医药重大科研专项基金资助项目（2018ZF006）

详细信息

作者简介:
刘艳红（1995～），女，云南大理人，在读硕士研究生，主要从事颗粒型疫苗佐剂研究工作

通讯作者:
鲁卫东，E-mail：13064244163@163.com

中图分类号: R392
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出版历程
- 收稿日期: 2020-12-10
- 刊出日期: 2021-04-09

Influence of DC-Chol Cationic Liposome Adjuvant on the Immune Effect of Tetravalent Influenza Vaccine

School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products，Kunming Medical University，Kunming Yunnan 650500，China

摘要

摘要: 目的观察阳离子脂质体DC-Chol修饰的四价流感疫苗的免疫原性。方法采用薄膜分散结合冻融-冻干法制备DC-Chol脂质体，检测其包封率。将小鼠分为七组，为DC-Chol脂质体疫苗组（250 µg/只、500 µg/只、750 µg/只、900 µg/只）、PBS组、中性脂质体组、疫苗原液组（n = 3），通过MTT法确定DC-Chol脂质体的最佳剂量。将小鼠分为四组，DC-Chol脂质体组、疫苗原液组、中性脂质体组以及PBS对照组，腹腔免疫小鼠，于第7天、14天、28天处死，MTT法和T淋巴细胞表面标记实验检测小鼠淋巴细胞增殖情况观察免疫原性。结果 MTT实验表明，DC-Chol修饰脂质体的最佳用量为500 µg/鼠（P < 0.05）；T淋巴细胞表面标记实验显示，DC-Chol 阳离子脂质体能明显增强脾淋巴细胞数（P < 0.05）。结论与中性脂质体相比，DC-Chol 阳离子脂质体作为四价流感疫苗的佐剂具有良好的免疫增强作用。
- 脂质体 /
- DC-Chol /
- 流感疫苗 /
- 细胞免疫
Abstract: Objective To study the immunogenicity of the tetravalent influenza vaccine modified by cationic liposome DC-Chol. Methods DC-Chol liposomes were prepared by thin film dispersion-freeze-thaw- freeze-drying method, and the encapsulation rate was determined. The mice were divided into seven groups, DC-Chol liposome vaccine group （250 µg/mouse, 500 µg/mouse, 750 µg/mouse, 900 µg/mouse）, PBS group, vaccine stock solution group, neutral liposome group （n = 3）Determine the optimal dose of DC-Chol liposomes by MTT method. The mice were divided into 4 groups, DC-Chol liposome group, vaccine stock solution group, neutral liposome group and PBS control group. Mice were immunized through the peritoneum and sacrificed on the 7, 14, and 28 days. The proliferation of mouse lymphocytes was detected by MTT method and T lymphocyte surface labeling experiment to study its immunogenicity. Results The MTT experiment showed that the optimal dose of DC-Chol modified liposomes was 500 μg/rat （P < 0.05）; T lymphocyte surface labeling experiments showed that DC-Chol cationic liposomes significantly increased the number of splenic lymphocytes （P < 0.05）. Conclusion Compared with neutral liposomes, DC-Chol cationic liposomes as an adjuvant of tetrovalent influenza vaccine has good immune enhancement effect.
- Liposome /
- DC-Chol /
- Influenza vaccine /
- Cellular immunity

HTML全文

图 1 不同剂量DC-Chol 7 d时的SI

与PBS比较，^*P < 0.05；与原液比较，^#P < 0.05。

Figure 1. SI of different doses of DC-Chol on 7 d

下载: 全尺寸图片幻灯片

图 2 一个免疫周期的SI

与PBS比较，^*P < 0.05；与原液比较，^#P < 0.05。

Figure 2. SI of an immune cycle

下载: 全尺寸图片幻灯片

图 3 14 d、28 d时 CD4⁺/CD8⁺比值

与PBS比较，^*P < 0.05；与原液比较，^#P < 0.05。

Figure 3. CD4⁺/CD8⁺ on 4 d 28 d

下载: 全尺寸图片幻灯片

表 1 不同含量的DC-Chol阳离子脂质体的包封率

Table 1. Encapsulation efficiency of DC-Chol cationic liposomes with different contents

DC-Chol含量（µg/鼠）包封率（%）

250 59.17
500 70.44
750 68.78
900 68.78

下载: 导出CSV

参考文献(18)

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