A Study on Diffusion Kurtosis Imaging in Acute Epilepsy Rats
-
摘要:
目的 探讨扩散峰度成像（diffusion kurtosis imaging，DKI）技术对急性期癫痫大鼠的研究价值。 方法 Li-Pilocarpine诱导急性期大鼠癫痫模型13只，作为模型组，选取9只正常大鼠作为对照组。采用DKI技术对2组研究对象进行扫描，测量双侧海马、双侧梨状皮层/内嗅皮层的部分各向异性（fractional anisotropy，FA）、平均扩散系数（mean diffusivity，MD）、平均峰度（mean kurtosis，MK）、平行峰度（axial kurtosis，K∥）、径向峰度（radial kurtosis，K⊥）。采用SPSS 17.0统计学软件进行统计学分析。 结果 与对照组相比，模型组大鼠双侧梨状皮层/内嗅皮层的MD降低，MK、K∥、K⊥升高，差异具有统计学意义（P < 0.05）。模型组大鼠双侧海马FA、MD、MK、K∥、K⊥的改变，差异无统计学意义（ P > 0.05）。 结论 DKI技术可以反映急性癫痫大鼠的微观病理改变，为急性期癫痫大鼠脑组织的病理生理改变提供理论依据。 Abstract:Objective To investigate brain abnormalities in rats with acute epilepsy by diffusional kurtosis imaging （DKI）. Methods MRI were performed on all rats. DKI images were obtained from 13 epilepsy rats and 9 normal controls. Fractional anisotropy （FA）, mean diffusivity （MD）, and mean kurtosis （MK）, axial kurtosis（K∥）, radial kurtosis（K⊥） of bilateral hippocampus, piriform cortex and entorhinal cortex were measured. SPSS 17.0 was used for statistical analysis. Results Compared with the control group, MD of bilateral piriform cortex/entorhinal cortex decreased, MK、K∥、K⊥ significantly increased in the model group （P < 0.05）. There were no statistically significant differences in FA, MD, MK, K∥ and K⊥ in the bilateral hippocampus of model group. Conclusion Using DKI to reveal the pathologic changes of rats with acute epilepsy provide theoretical basis for the pathophysiological changes of brain tissues of rats with acute epilepsy. -
Key words:
- Epilepsy /
- Rat /
- Diffusion kurtosis imaging
-
图 1 DKI在急性癫痫大鼠脑组织的测量位置
A：FA图；B：MD图；C：MK图；D：K∥图；E：K⊥图；F：T2WI。
Figure 1. Brain regions measured by DKI in acute epileptic rats
表 1 对照组和模型组大鼠左侧梨状皮层/内嗅皮层的DKI值比较（ $\bar x \pm s $）
Table 1. Comparisons of DKI values of the left piriform cortex/entorhinal cortex between control group and model group （ $\bar x\pm s $）
DKI指标值 FA MD MK K∥ K⊥ 对照组 0.16 ± 0.03 1.62 ± 0.12 0.93 ± 0.07 0.81 ± 0.06 1.00 ± 0.09 模型组 0.17 ± 0.03 1.21 ± 0.53 1.3 ± 0.44 1.11 ± 0.38 1.41 ± 0.48 t −1.304 2.44 −2.73 −2.632 −2.773 P 0.207 0.032* 0.020* 0.024* 0.019* *P < 0.05。 
下载: 导出CSV
表 2 正常对照组和模型组大鼠右侧梨状皮层/内嗅皮层的DKI值比较（ $ \bar x \pm s$）
Table 2. Comparisons of DKI values of the right piriform cortex/entorhinal cortex between control group and model group （ $\bar x \pm s $）
DKI指标值 FA MD MK K∥ K⊥ 对照组 0.14 ± 0.02 1.69 ± 0.08 0.89 ± 0.04 0.78 ± 0.04 0.95 ± 0.05 模型组 0.15 ± 0.02 1.27 ± 0.56 1.26 ± 0.46 1.09 ± 0.39 1.36 ± 0.5 t −1.13 2.46 −2.668 −2.633 −2.685 P 0.270 0.033* 0.023* 0.025* 0.023* *P < 0.05。
下载: 导出CSV
表 3 正常对照组和模型组大鼠左侧海马的DKI值比较（ $\bar x \pm s $）
Table 3. Comparisons of DKI values of left hippocampus between normal control group and model group （ $\bar x \pm s $）
DKI指标值 FA MD MK K∥ K⊥ 对照组 0.15 ± 0.04 1.62 ± 0.10 0.86 ± 0.23 0.80 ± 0.06 0.96 ± 0.1 模型组 0.13 ± 0.02 1.60 ± 0.5 0.87 ± 0.04 0.76 ± 0.05 0.93 ± 0.04 t 1.59 0.098 −0.216 1.557 0.98 P 0.127 0.923 0.831 0.135 0.33
下载: 导出CSV
表 4 正常对照组和模型组大鼠右侧海马的DKI值比较（ $\bar x \pm s $）
Table 4. Comparisons of DKI values of right hippocampus between control group and model group （ $\bar x\pm s $）
DKI指标值 FA MD MK K∥ K⊥ 对照组 0.13 ± 0.02 1.65 ± 0.11 0.91 ± 0.07 0.77 ± 0.05 0.98 ± 0.08 模型组 0.15 ± 0.03 1.56 ± 0.49 0.89 ± 0.05 0.76 ± 0.04 0.97 ± 0.06 t −1.68 0.589 0.413 0.538 0.41 P 0.115 0.563 0.684 0.596 0.68
下载: 导出CSV
-
[1] Singh A,Trevick S. The epidemiology of global epilepsy[J]. Neurol Clin,2016,34(4):837-847. doi: 10.1016/j.ncl.2016.06.015 [2] Jensen J H,Helpern J A. MRI quantification of non-Gaussian water diffusion by kurtosis analysis[J]. NMR Biomed,2010,23(7):698-710. doi: 10.1002/nbm.1518 [3] Fieremans E,Jensen J H,Helpern J A. White matter characterization with diffusional kurtosis imaging[J]. Neuroimage,2011,58(1):177-188. doi: 10.1016/j.neuroimage.2011.06.006 [4] Glenn G R,Jensen J H,Helpern J A,et al. Epilepsy-related cytoarchitectonic abnormalities along white matter pathways[J]. Journal of Neurology,Neurosurgery,and Psychiatry,2016,87(9):930-936. doi: 10.1136/jnnp-2015-312980 [5] Surova Y,Nilsson M,Lampinen B,et al. Alteration of putaminal fractional anisotropy in parkinson's disease:A longitudinal diffusion kurtosis imaging study[J]. Neuroradiology,2018,60(3):247-254. doi: 10.1007/s00234-017-1971-3 [6] Karlsen R H,Einarsen C,Moe H K,et al. Diffusion kurtosis imaging in mild traumatic brain injury and postconcussional syndrome[J]. Journal of Neuroscience Research,2019,97(5):568-581. doi: 10.1002/jnr.24383 [7] 龚霞蓉,吴昆华,赵英,等. 锂-匹罗卡品诱导癫痫大鼠急性期MRI表现[J]. 中华神经医学杂志,2019,18(10):1035-1037. [8] Jones D K,Knösche T R,Turner R. White matter integrity,fiber count,and other fallacies:The do's and don'ts of diffusion MRI[J]. Neuroimage,2013,73(6):239-254. [9] Jensen J H,Helpern J A,Tabesh A. Leading non-gaussian corrections for diffusion orientation distribution function[J]. NMR Biomed,2014,27(2):202-211. doi: 10.1002/nbm.3053 [10] Jensen J H,Helpern J A,Ramani A,et al. Diffusional kurtosis imaging:The quantification of non-gaussian water diffusion by means of magnetic resonance imaging[J]. Magn Reson Med,2005,53(6):1432-1440. doi: 10.1002/mrm.20508 [11] Lazar M,Jensen J H,Xuan L,et al. Estimation of the orientation distribution function from diffusional kurtosis imaging[J]. Magn ResonMed,2008,60(4):774-781. doi: 10.1002/mrm.21725 [12] Hori M,Fukunaga I,Masutani Y,et al. New diffusion metrics for spondylotic myelopathy at an early clinical stage[J]. Eur Radiol,2012,22(8):1797-1802. doi: 10.1007/s00330-012-2410-9 [13] Veraart J,Poot D H,Van Hecke W,et al. More accurate estimation of diffusion tensor parameters using diffusion kurtosis imaging[J]. Magn Reson Med,2011,65(1):138-145. [14] Choy M, Cheung K K, Thomas D L, et al. Quantitative MRI predicts status epilepticus-induced hippocampal injury in the lithium-pilocarpine rat model[J], Epilepsy Research, 2010, 88（2-3）: 221-230. [15] 曾丁巳,肖新兰. 扩散峰度成像在中枢神经系统的应用[J]. 临床放射学杂志,2011,30(9):1400-1402. [16] 陈建新,付丽媛,梁永刚,等. 颞叶癫痫患者发作间期内侧颞叶磁 共振动态磁敏感对比增强灌注成像研究[J]. 中国CT和MRI杂志,2017,15(1):7-10. [17] 丁秀芳. 癫痫持续状态模型中海马神经元损伤及其可能机制的研究[D]. 济南: 山东大学硕士论文, 2016. [18] Valle-Dorado M G,Santana-Gómez C E,Orozco-Suárez S A,et al. The mast cell Stabilizer sodium cromoglycate reduces histamine release and status Epilepticus-induced neuronal damage in the rat hippocampus[J]. Neuropharmacology,2015,92(5):49-55. [19] Engelhorn T,Doerfler A,Weise J,et al. Cerebral perfusion alterations during the acute phase of experimental generalized status epilepticus:Prediction of survival by using perfusion-weighted MR imaging and histopathology[J]. AJNR,2005,26(6):1563-1570. -
点击查看大图
计量
- 文章访问数: 3403
- HTML全文浏览量: 2102
- PDF下载量: 11
- 被引次数: 0
