长链非编码RNA APTR、HEIH、FAS-ASA1、FAM83H-AS1、DICER1-AS1、PR-lncRNA在肺癌中的表达

张腾飞; 何越峰; 张磊; 张莉; 杨凯云; 刘莲清; 谭慧

doi:10.12259/j.issn.2095-610X.S20210409

长链非编码RNA APTR、HEIH、FAS-ASA1、FAM83H-AS1、DICER1-AS1、PR-lncRNA在肺癌中的表达

doi: 10.12259/j.issn.2095-610X.S20210409

1.
昆明医科大学第三附属医院胸外一科，云南昆明　650118
2.
昆明医科大学公共卫生学院，云南昆明　650500

基金项目: 国家自然科学基金资助项目（81860572）；云南省卫生科技计划基金资助项目（2016NS108）

详细信息

作者简介:
张腾飞（1995年～），男，河南西平人，在读硕士研究生，主要从事肿瘤学研究工作

通讯作者:
谭慧，E-mail：tanhuikm@163.com

中图分类号: R734.2
计量
- 文章访问数: 3633
- HTML全文浏览量: 2536
- PDF下载量: 109
- 被引次数: 4
出版历程
- 收稿日期: 2020-12-28
- 刊出日期: 2021-04-01

Expression of Long Non-coding RNA APTR，HEIH，FAS-ASA1，FAM83H-AS1，DICER1-AS1，PR-lncRNA in Lung Cancer

1.
Dept. of Thoracic Surgery，The 3rd Affiliated Hospital of Kunming Medical University，Kunming Yunnan，650118
2.
School of Public Health，Kunming Medical University，Kunming Yunnan 650500，China

摘要

摘要: 目的探讨长链非编码RNA（Long non-coding RNA，IncRNA）APTR、HEIH、FAS-ASA1、FAM83H-AS1、DICER1-AS1、PR-lncRNA在肺癌患者中的表达。方法收集昆明医科大学第三附属医院肺癌手术患者的癌组织，癌旁正常组织及肺癌淋巴结转移组织，利用TRI z o l提取总 RNA，再用SYBR Green实时荧光定量 PCR检测每种基因的RNA相对表达量。结果 APTR在淋巴组织中表达升高（P < 0.05），HEIH在淋巴组织中表达下降（P < 0.05），FAM83H-AS1和、PR-lncRNA在肺癌组织中表达升高（P < 0.05）。结论 APTR在淋巴组织中高表达，可能有促进肺癌淋巴结转移的作用；FAM83H-AS1及PR-lncRNA癌组织中表达上调，可能会促进肺癌的发生发展。
- 肺癌 /
- 长链非编码RNA /
- 表达 /
- 癌基因
Abstract: Objective To investigate the expression of long non-coding RNA （IncRNA） APTR, HEIH, FAS-ASA1, FAM83H-AS1, DICER1-AS1 and PR-lncRNA in lung cancer patients. METHODS Total RNA was extracted by TRI Z O L from cancer tissues, adjacent normal tissues and lymph node metastases of lung cancer patients in the Third Affiliated Hospital of Kunming Medical University. The relative RNA expression of each gene was detected by SYBR GR e e N real-time quantitative fluorescence PCR. RESULTS The expression of APTR was increased in lymphoid tissues （P < 0.05）, the expression of HEIH was decreased in lymphoid tissues （P < 0.05）, and the expression of FAM83H-AS1 and PR-lncRNA were increased in lung cancer tissues （P < 0.05）. Conclusion Increased expression of APTR in lymphoid tissue may promote lymph node metastasis of lung cancer; increased expression of FAM83H-AS1 and PR-lncRNA in cancer tissue may promote the occurrence and development of lung cancer.
- Lung cancer /
- LncRNA /
- Expression /
- Oncogene

HTML全文

表 1 每种基因在不同部位的表达量（$\bar{x} \pm s$）

Table 1. The genes expression in different tissues （$\bar{x} \pm s$）

基因	正常组织	癌组织	淋巴组织
APTR	8.38 ± 2.83	9.72 ± 4.36^*	12.05 ± 6.06
HEIH	10.81 ± 1.97	11.13 ± 2.17^*	8.67 ± 2.70
FAS-ASA1	6.01 ± 3.73	7.45 ± 3.73	6.79 ± 2.74
FAM83H-AS1	7.07 ± 2.70	10.02 ± 3.89^*	8.38 ± 2.80
DICER1-AS1	8.61 ± 2.71	10.30 ± 3.80	9.83 ± 2.38
PR-lncRNA	7.12 ± 2.76	9.36 ± 3.96^*	9.13 ± 3.60
与正常组织相比，^*P < 0.05。

下载: 导出CSV

表 2 各基因的表达与ki-67 Spearman等级相关结果

Table 2. The correlation between genes and Ki-67 Spearman rank

基因	r	P
APTR	0.05	0.9081
HEIH	−0.07	0.0687
FAS-ASA1	−0.56	0.1463
FAM83H-AS1	−0.52	0.1915
DICER1-AS1	−0.85	0.0075^*
PR-lncRNA	−0.10	0.8215
^*P < 0.05。

下载: 导出CSV

参考文献(20)

[1]	Esfandi F,Taheri M,Omrani M D,et al. Expression of long non-coding RNAs(lncRNAs)has been dysregulated in non-small cell lung cancer tissues[J]. BMC Cancer,2019,9(1):222.
[2]	Zhang Y,Li Z,Zhang Y,et al. Molecular mechanism of HEIH and HULC in the proliferation and invasion of hepatoma cells[J]. Int J Clin Exp Med,2015,8(8):12956-12962.
[3]	Lu S,Dong W,Zhao P,et al. lncRNA FAM83H-AS1 is associated with the prognosis of colorectal carcinoma and promotes cell proliferation by targeting the Notch signaling pathway[J]. Oncol Lett,2018,15(2):1861-1868.
[4]	Barr J A,Hayes K E,Brownmiller T,et al. Long non-coding RNA FAM83H-AS1 is regulated by human papillomavirus 16 E6 independently of p53 in cervical cancer cells[J]. Sci Rep,2019,9(1):3662. doi: 10.1038/s41598-019-40094-8
[5]	Cui C,Zhai D,Cai L,et al. Long noncoding RNA HEIH promotes colorectal cancer tumorigenesis via Counteracting miR-939‒mediated transcriptional repression of Bcl-xL[J]. Cancer Res Treat,2018,50(3):992-1008. doi: 10.4143/crt.2017.226
[6]	Guan H,Shang G,Cui Y,et al. Long noncoding RNA APTR contributes to osteosarcoma progression through repression of miR-132-3p and upregulation of yes-associated protein 1[J]. J Cell Physiol,2019,234(6):8998-9007. doi: 10.1002/jcp.27572
[7]	Gu Z,Hou Z,Zheng L,et al. LncRNA DICER1-AS1 promotes the proliferation,invasion and autophagy of osteosarcoma cells via miR-30b/ATG5[J]. Biomed Pharmacother,2018,104(8):110-118.
[8]	Sergio Torres Bayona,Paula Aldaz,Jaione Auzmendi Iriarte,et al. PR-LncRNA signature regulates glioma cell activity through expression of SOX factors[J]. Sci Rep,2018,24(1):12746.
[9]	Sayad A,Hajifathali A,Hamidieh A A,et al. Fas-antisense long noncoding RNA and acute myeloid leukemia:Is there any relation[J]. Asian Pac J Cancer Prev,2018,19(1):45-48.
[10]	S W,Zhou,B B,Su,Y,Zhou. Expression of FAS-AS1 in breast cancer and its effect on the host gene sFas.[J]. Zhonghua Yi Xue Za Zhi,2016,96(12):949-953.
[11]	Hu X H,Dai J,Shang H L,et al. High levels of long non-coding RNA DICER1-AS1 are associated with poor clinical prognosis in patients with osteosarcoma[J]. Eur Rev Med Pharmacol Sci,2018,22(22):7640-7645.
[12]	Shan H,Yang Y,Zhu X,et al. FAM83H-AS1 is associated with clinical progression and modulates cell proliferation,migration,and invasion in bladder cancer[J]. J Cell Biochem,2019,120(3):4687-4693. doi: 10.1002/jcb.27758
[13]	Dou Q,Xu Y,Zhu Y,et al. LncRNA FAM83H-AS1 contributes to the radioresistance,proliferation,and metastasis in ovarian cancer through stabilizing HuR protein[J]. Eur J Pharmacol,2019,852(5):134-141.
[14]	Yang F,Lv S X,Lv L,et al. Identification of lncRNA FAM83H-AS1 as a novel prognostic marker in luminal subtype breast cancer[J]. Onco Targets Ther,2016,15(9):7039-7045.
[15]	Yang L,Xu L,Wang Q,et al. Dysregulation of long non-coding RNA profiles in human colorectal cancer and its association with overall survival[J]. Oncol Lett,2016,12(5):4068-4074. doi: 10.3892/ol.2016.5138
[16]	Zhang J,Feng S,Su W,et al. Overexpression of FAM83H-AS1 indicates poor patient survival and knockdown impairs cell proliferation and invasion via MET/EGFR signaling in lung cancer[J]. Sci Rep,2017,15(7):42819.
[17]	Yang F,Zhang L,Huo X S,et al. Long noncoding RNA high expression in hepatocellular carcinoma facilitates tumor growth through enhancer of zeste homolog 2 in humans[J]. Hepatology,2011,54(5):1679-1689. doi: 10.1002/hep.24563
[18]	杨哲锋,李娟,马志元,等. 血浆LncRNA-HEIH表达水平与肝癌发病风险的关联分析[J]. 现代肿瘤医学,2015,23(01):80-84. doi: 10.3969/j.issn.1672-4992.2015.01.23
[19]	He Y,Meng X M,Huang C,et al. Long noncoding RNAs:Novel insights into hepatocelluar carcinoma[J]. Cancer Lett,2014,344(1):20-27. doi: 10.1016/j.canlet.2013.10.021
[20]	Negishi M,Wongpalee S P,Sarkar S,et al. A new lncRNA,APTR,associates with and represses the CDKN1A/p21 promoter by recruiting polycomb proteins[J]. PLoS One,2014,18(4):e95216.

相关文章(20)

[1]	杨倩蓉, 曹雪, 杨娇, 赵燕秋, 沈楠, 孙娅娇. 肺癌患者病耻感现状及影响因素, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20250108
[2]	张江, 赵喜娟, 吴江, 杨秉坤, 杨妮, 周丽萍. 肺癌放疗患者衰弱现状及影响因素分析, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20250218
[3]	李杨, 刘容, 刘朝敏, 徐龙玉, 张文静. 基于生物信息学识别与肺癌患者预后和免疫微环境相关的铜死亡基因, 昆明医科大学学报.
[4]	王缨, 傅聪, 傅颖. 肺癌患者血清LDH、CysC、PWR水平检测意义, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20240424
[5]	陈雪雅, 许金美, 李智, 梁燕, 姚宇峰, 何凤权, 严志凌. HOXD-AS2、MIR3142HG基因多态性与宫颈上皮内瘤变的相关性, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20241103
[6]	谢飞飞, 辛隐子, 徐敏, 李景涵, 王伟. 长链非编码RNA在软骨发育及骨关节炎中作用机制的研究进展, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20241001
[7]	顾君, 何泽喜, 栾婷, 王海峰, 王剑松, 丁明霞. 外泌体长链非编码RNA在膀胱癌中的研究进展, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220110
[8]	郭方圆, 李文亮, 徐凡, 魏向群. 非编码RNA 在宫颈癌组织中的表达及临床意义, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220527
[9]	李梦仙, 俞建昆, 温艳萍, 吴翰欣, 高凌, 刘晓晓, 邰文琳. 剪接因子SRSF9在肺癌中的表达量变化及意义, 昆明医科大学学报.
[10]	阎婷婷, 田丹, 李新, 俎云芬, 李崇阳, 尚学琴. MicroRNA Let-7a与p53基因在肺癌中表达的相关性, 昆明医科大学学报.
[11]	范敏娟, 钟云华, 沈雯, 袁开芬, 赵国厚, 王蜀昆, 温林俏. 肺癌中热休克转录因子2促进热休克蛋白的表达, 昆明医科大学学报.
[12]	吕超绍. rhIFN-γ对白血病K562细胞CD123表达的影响, 昆明医科大学学报.
[13]	赵杰. 半乳糖凝集素3在COPD和肺癌中的表达及其意义, 昆明医科大学学报.
[14]	喻丽. TNIP1真核表达载体的构建及表达, 昆明医科大学学报.
[15]	曾玉剑. Toll-like Receptor 9 在大鼠胰腺表达及与大鼠急性胰腺炎相关性的研究, 昆明医科大学学报.
[16]	肖肖. YB-1在中晚期宫颈癌组织中的表达及其临床意义, 昆明医科大学学报.
[17]	魏洁. Twist在中晚期宫颈癌组织中的表达及临床意义研究, 昆明医科大学学报.
[18]	杨建华. Tenascin-W在结直肠癌中的表达及临床意义, 昆明医科大学学报.
[19]	杜兴华. ALX4基因在肿瘤中的甲基化与表达, 昆明医科大学学报.
[20]	胡振良. 重组蛋氨酸裂解酶原核体系的表达及纯化, 昆明医科大学学报.

施引文献

期刊类型引用(4)

1.	李景峰，吴淑君，李继德. AGAP2-AS1在非小细胞肺癌组织中的表达及临床意义. 实用癌症杂志. 2023(08): 1253-1255 . 百度学术
2.	丁建花，韩向春. IncRNA在肝细胞癌中的重要作用及临床应用. 现代诊断与治疗. 2023(24): 3669-3671 . 百度学术
3.	刘晨，姚俊朝，张祥. LncRNA FAS-AS1通过抑制过度的自噬促进胶质瘤细胞对顺铂的敏感性. 药物分析杂志. 2022(02): 294-301 . 百度学术
4.	程明，王伟伟，吕世琪，高勇. CEA、CA125、CYFRA21-1、NSE及SCC对NSCLC诊断和化疗效果评估的临床价值. 分子诊断与治疗杂志. 2022(07): 1099-1103 . 百度学术