长链非编码RNA APTR、HEIH、FAS-ASA1、FAM83H-AS1、DICER1-AS1、PR-lncRNA在肺癌中的表达

张腾飞; 何越峰; 张磊; 张莉; 杨凯云; 刘莲清; 谭慧

doi:10.12259/j.issn.2095-610X.S20210409

长链非编码RNA APTR、HEIH、FAS-ASA1、FAM83H-AS1、DICER1-AS1、PR-lncRNA在肺癌中的表达

doi: 10.12259/j.issn.2095-610X.S20210409

1.
昆明医科大学第三附属医院胸外一科，云南昆明　650118
2.
昆明医科大学公共卫生学院，云南昆明　650500

基金项目: 国家自然科学基金资助项目（81860572）；云南省卫生科技计划基金资助项目（2016NS108）

详细信息

作者简介:
张腾飞（1995年～），男，河南西平人，在读硕士研究生，主要从事肿瘤学研究工作

通讯作者:
谭慧，E-mail：tanhuikm@163.com

中图分类号: R734.2
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出版历程
- 收稿日期: 2020-12-28
- 刊出日期: 2021-04-01

Expression of Long Non-coding RNA APTR，HEIH，FAS-ASA1，FAM83H-AS1，DICER1-AS1，PR-lncRNA in Lung Cancer

1.
Dept. of Thoracic Surgery，The 3rd Affiliated Hospital of Kunming Medical University，Kunming Yunnan，650118
2.
School of Public Health，Kunming Medical University，Kunming Yunnan 650500，China

摘要

摘要: 目的探讨长链非编码RNA（Long non-coding RNA，IncRNA）APTR、HEIH、FAS-ASA1、FAM83H-AS1、DICER1-AS1、PR-lncRNA在肺癌患者中的表达。方法收集昆明医科大学第三附属医院肺癌手术患者的癌组织，癌旁正常组织及肺癌淋巴结转移组织，利用TRI z o l提取总 RNA，再用SYBR Green实时荧光定量 PCR检测每种基因的RNA相对表达量。结果 APTR在淋巴组织中表达升高（P < 0.05），HEIH在淋巴组织中表达下降（P < 0.05），FAM83H-AS1和、PR-lncRNA在肺癌组织中表达升高（P < 0.05）。结论 APTR在淋巴组织中高表达，可能有促进肺癌淋巴结转移的作用；FAM83H-AS1及PR-lncRNA癌组织中表达上调，可能会促进肺癌的发生发展。
- 肺癌 /
- 长链非编码RNA /
- 表达 /
- 癌基因
Abstract: Objective To investigate the expression of long non-coding RNA （IncRNA） APTR, HEIH, FAS-ASA1, FAM83H-AS1, DICER1-AS1 and PR-lncRNA in lung cancer patients. METHODS Total RNA was extracted by TRI Z O L from cancer tissues, adjacent normal tissues and lymph node metastases of lung cancer patients in the Third Affiliated Hospital of Kunming Medical University. The relative RNA expression of each gene was detected by SYBR GR e e N real-time quantitative fluorescence PCR. RESULTS The expression of APTR was increased in lymphoid tissues （P < 0.05）, the expression of HEIH was decreased in lymphoid tissues （P < 0.05）, and the expression of FAM83H-AS1 and PR-lncRNA were increased in lung cancer tissues （P < 0.05）. Conclusion Increased expression of APTR in lymphoid tissue may promote lymph node metastasis of lung cancer; increased expression of FAM83H-AS1 and PR-lncRNA in cancer tissue may promote the occurrence and development of lung cancer.
- Lung cancer /
- LncRNA /
- Expression /
- Oncogene

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表 1 每种基因在不同部位的表达量（$\bar{x} \pm s$）

Table 1. The genes expression in different tissues （$\bar{x} \pm s$）

基因	正常组织	癌组织	淋巴组织
APTR	8.38 ± 2.83	9.72 ± 4.36^*	12.05 ± 6.06
HEIH	10.81 ± 1.97	11.13 ± 2.17^*	8.67 ± 2.70
FAS-ASA1	6.01 ± 3.73	7.45 ± 3.73	6.79 ± 2.74
FAM83H-AS1	7.07 ± 2.70	10.02 ± 3.89^*	8.38 ± 2.80
DICER1-AS1	8.61 ± 2.71	10.30 ± 3.80	9.83 ± 2.38
PR-lncRNA	7.12 ± 2.76	9.36 ± 3.96^*	9.13 ± 3.60
与正常组织相比，^*P < 0.05。

下载: 导出CSV

表 2 各基因的表达与ki-67 Spearman等级相关结果

Table 2. The correlation between genes and Ki-67 Spearman rank

基因	r	P
APTR	0.05	0.9081
HEIH	−0.07	0.0687
FAS-ASA1	−0.56	0.1463
FAM83H-AS1	−0.52	0.1915
DICER1-AS1	−0.85	0.0075^*
PR-lncRNA	−0.10	0.8215
^*P < 0.05。

下载: 导出CSV

参考文献(20)

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