Effects of Different Concentrations of Magnesium Ions on Endothelial Progenitor Cells in Women with Gestational Diabetes
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摘要:
目的 探究不同浓度镁离子干预对妊娠糖尿病（gestaional diabetes mellitus，GDM）妇女内皮祖细胞（endothelial progenitor cells，EPCs）的影响。 方法 选取2016年5月至2020年3月的204例妊娠期糖尿病患者为观察组，并选取同期住院的204例正常妊娠妇女为对照组，采用随机数字表法将观察组分为3组，并分别给予浓度不同的镁离子进行干预，A组（4 mmol/L）、B组（8 mmol/L）、C组（16 mmol/L）；采用同样的方法将对照组分为3组，并分别给予浓度不同的镁离子进行干预，a组（4 mmol/L）、b组（8 mmol/L）、c组（16 mmol/L）。6组患者中每组均为68例。6组患者中每位孕妇均入组进行脐血分离培养，每例均为6孔细胞入组。对EPCs细胞增殖、凋亡、粘附、细胞周期进行比较。 结果 观察组3组妇女的增殖能力均低于对照组3组，且B组优于A组，A组优于C组；b组优于a组，a组优于c组；以正常人群的细胞凋亡率为100%，观察组3组妇女的增殖能力均高于对照组3组，且B组低于A组，A组低于C组，b组低于a组，a组低于c组，差异有统计学有意义（P < 0.05）。使用Image J软件计算，观察组3组妇女的细胞面积和细胞密度均低于对照组3组，且B组高于A组，A组高于C组，b组高于a组，a组高于c组，差异有统计学有意义（P < 0.05）。观察组3组妇女细胞生长停滞在G0/G1期少于对照组3组，且B组优于A组，A组优于C组，b组优于a组，a组优于c组，差异有统计学有意义（P < 0.05）。 结论 8 mmol/L的镁离子可能与妊娠期妇女EPCs的增殖和粘附相关，进而抑制凋亡，改善细胞周期，且对GDM妇女的作用更显著，进一步研究后可用于帮助改善GDM孕产妇的妊娠结局。 Abstract:Objective To explore the effects of different concentrations of magnesium ion intervention on endothelial progenitor cells （endothelial progenitor cells, EPCs） in women with gestational diabetes （gestaional diabetes mellitus, GDM）. Methods 204 cases of gestational diabetes mellitus from May 2016 to March 2020 were selected as the observation group, and 204 cases of normal pregnant women hospitalized during the same period were selected as the control group. The observation group was divided into group A （4 mmol/L）, group B （8 mmol/L） and group C （16 mmol/L） by random number table method, and were treated with different concentrations of magnesium ions. Using the same method, the control group was divided into group a （4 mmol/L）, group b （8 mmol/L） and group c （16 mmol/L）, and was also given different concentrations of magnesium ions. There were 68 patients in each of the six groups. In the six groups of patients, each pregnant woman was enrolled for cord blood isolation and culture, and each case was enrolled with 6-well cells. The cell proliferation, apoptosis, adhesion and cell cycle of EPCs were compared. Results The proliferative capacity of the three groups of women in the observation group was lower than the those of control group, with group B better than group A, group A better than group C, group b better than group a, and group a better than group c. The apoptosis rate is 100% in normal population. The proliferative capacity of the three groups of women in the observation group is higher than that in the control group, with group B lower than group A, group A lower than group C, group b lower than group a, group a lower than group c, the difference is statistically significant （P < 0.05）. According to the calculation using Image J software, the cell area and cell density in the observation group were lower than those in the control group, with group B higher than group A, group A higher than group C, group b higher than group a, and group a higher than group c; the difference is statistically significant （P < 0.05）. The occurrence of G0 / G1 phase cell cycle arrest in the observation group was lower than that in control group, with group B better than group A, group A better than group C, group b better than group a, and group a better than group c, the difference is statistically significant （P < 0.05）. Conclusion 8 mmol/L magnesium ion may be associated with the proliferation and adhesion of EPCs in pregnant women, thus inhibiting apoptosis and upregulating cell cycle, and its effect on women with GDM is more evident, which can be used to help improve the pregnancy outcome of pregnant women with GDM after further study. -
Key words:
- Gestational diabetes /
- Endothelial progenitor cells /
- Magnesium ion
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图 2 各组细胞面积比较
与A组比较，*P < 0.05；与a组比较，#P < 0.05。
Figure 2. Comparison of cell area among each group
图 3 各组细胞密度比较
与A组比较，*P < 0.05；与a组比较，#P < 0.05。
Figure 3. Comparison of cell density among each group
图 5 各组细胞周期比较
与A组比较，*P < 0.05；与a组比较，#P < 0.05。
Figure 5. Comparison of cell cycle among each group
表 1 对EPCs细胞增殖和凋亡的影响（
$\bar x \pm s$ ）Table 1. Effects on proliferation and apoptosis of EPCs （
$\bar x \pm s$ ）分组 n CCK-8（λ = 450 mm） 凋亡率（%） A组 68 0.20 ± 0.05 279.07 ± 59.84 B组 68 0.23 ± 0.06 214.43 ± 57.76 C组 68 0.18 ± 0.03 317.65 ± 63.32 a组 68 0.28 ± 0.04 121.67 ± 34.52 b组 68 0.30 ± 0.05 95.78 ± 21.34 c组 68 0.25 ± 0.03 156.64 ± 30.09 F 73.541 53.652 P < 0.001 < 0.001 
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表 2 对EPCs细胞粘附的影响（
$\bar x \pm s$ ）Table 2. Effects on EPCs cell adhesion （
$\bar x \pm s$ ）分组 n 细胞面积（μm2） 细胞密度（cells/mm2） A组 68 5744.38 ± 1022.47 73.32 ± 7.10 B组 68 6193.26 ± 983.77 76.45 ± 6.31 C组 68 5489.05 ± 883.21 70.78 ± 5.57 ` a组 68 7348.79 ± 1143.25 81.06 ± 8.22 b组 68 7548.43 ± 1290.83 82.90 ± 7.43 c组 68 7054.27 ± 1075.43 79.13 ± 8.63 F 63.891 43.857 P < 0.001 < 0.001
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表 3 对EPCs细胞周期的影响（
$\bar x \pm s$ ）Table 3. Effects on EPCs cell cycle （
$\bar x \pm s$ ）分组 n G0/G1 S G2/M A组 68 81.33 ± 2.78 13.36 ± 2.08 3.92 ± 1.43 B组 68 79.19 ± 3.07 15.64 ± 2.77 4.35 ± 1.70 C组 68 84.52 ± 3.14 11.97 ± 1.88 3.65 ± 1.23 a组 68 72.13 ± 2.56 22.98 ± 1.95 5.06 ± 0.78 b组 68 67.56 ± 2.61 26.24 ± 3.17 5.67 ± 1.32 c组 68 77.35 ± 3.56 17.89 ± 2.03 4.98 ± 1.56 F 49.976 63.532 17.037 P < 0.001 < 0.001 < 0.001
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