Effect of Acetone in Human Blood on Ethanol Detection Results
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摘要:
目的 研究血液样品中丙酮对乙醇定性定量结果的影响。 方法 采用顶空气相色谱法（双柱双检测器）测定600例涉嫌酒后或醉酒驾车驾驶员血液样品中的乙醇及丙酮。 结果 色谱柱1（DB-ALC1）可将内标叔丁醇和丙酮有效分离，而色谱柱2（DB-ALC2）不能将丙酮与内标叔丁醇分离，干扰乙醇定量结果的准确性。采用GC-MS法对疑似含有丙酮的部分血样进行确证，均检出丙酮成分，顶空气相色谱法对113例血样中丙酮进行定量分析，血样中丙酮含量最高为6.55 mg/100mL。 结论 法医学鉴定中应关注丙酮对乙醇检测结果的影响，为准确判定酒驾、醉驾案（事）件性质，应采用双柱双检测器进行乙醇定性判定，在血液样品中存在丙酮干扰时，采用DB-ALC1色谱柱测定的平均值作为定量结果。 Abstract:Objective To study the effect of acetone in blood samples on the qualitative and quantitative results of ethanol. Methods Headspace gas chromatography （double column and double detector） was used to determine ethanol and acetone in blood samples of 600 suspected drunk drivers or driving after drinking drivers. Results In column 1 （DB-ALC1）, internal standard tert-butanol and acetone could be separated effectively; while in column 2 （DB-ALC2）, acetone could not be separated from the internal standard tert-butanol, which interferes with the accuracy of the ethanol quantitative results. Some blood samples suspected to contain acetone tested in this study were confirmed by GC-MS, and all acetone components were detected positive. The acetone concentration in 113 blood samples were detected by GC. The highest acetone content in the blood samples was 6.55mg/100mL. Conclusion In forensic medical identification, attention should be paid to the influence of acetone on the detection results of ethanol. In order to accurately identify the fact of driving after drinking and drunk driving cases, double-column dual detectors should be used for qualitative determination of ethanol. In the presence of acetone, the mean value of DB-ALC1 column was used as quantitative results. -
Key words:
- Headspace gas chromatography /
- Blood /
- Ethanol /
- Acetone
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图 1 实际案例静脉血顶空气相色谱图
a：柱1色谱图，b：柱2色谱图。
Figure 1. The gas chromatogram of venous blood in the actual cases
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