真核翻译起始因子-5A2在肝内胆管癌中的表达及意义

杨少华; 廖周俊; 胡晟; 洪茂林; 宋志美; 危群; 张小文

doi:10.12259/j.issn.2095-610X.S20210505

真核翻译起始因子-5A2在肝内胆管癌中的表达及意义

doi: 10.12259/j.issn.2095-610X.S20210505

1.
昆明医科大学第二附属医院肝胆胰外科二病区
2.
病理科，云南昆明　650101

基金项目: 国家自然科学基金资助项目（81760430）

详细信息

作者简介:
杨少华（1988～），男，陕西渭南人，在读博士研究生，主要从事肝胆胰外科疾病的临床与基础研究工作

通讯作者:
张小文，E-mail：zhangxiaowenlu@hotmail.com

中图分类号: R735.8
计量
- 文章访问数: 3248
- HTML全文浏览量: 2170
- PDF下载量: 21
- 被引次数: 0
出版历程
- 收稿日期: 2021-01-04
- 网络出版日期: 2021-06-02
- 刊出日期: 2021-05-20

The Expression and Significance of Eukaryotic Translation Initiation Factor-5A2 in Intrahepatic Cholangiocarcinoma

1.
Dept. of Hepatobiliary and Pancreatic Surgery
2.
Dept. of Pathology，The Second Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

摘要

摘要: 目的研究真核翻译起始因子-5A2（eukaryotic translation initiation factor 5A2，eIF5A2）在肝内胆管癌中的表达及与神经侵犯和周围脂肪组织浸润的相关性。方法收集昆明医科大学第二附属医院肝胆胰外科2013年10月至2020年10月156例肝内胆管癌患者术后石蜡切块，收集同期30例肝血管瘤患者部分肝切除的正常肝组织作为对照组，采用免疫组化的方法检测eIF5A2的表达水平，并收集病理资料，分析eIF5A2与神经侵犯和周围脂肪组织浸润的相关性。结果 156例肝内胆管癌组织切片中eIF5A2高表达95例（60.9%），其在正常肝组织胆管上皮高表达率为13.3%，eIF5A2的表达与神经侵犯和周围脂肪组织浸润显著相关（P < 0.05）。结论 eIF5A2在肝内胆管癌中高表达，与神经侵犯和周围脂肪组织浸润显著相关。
- 肝内胆管癌 /
- 真核翻译起始因子-5A2 /
- 免疫组织化学染色
Abstract: Objective To study the expression of eukaryotic translation initiation factor 5A2 （eIF5A2） in intrahepatic cholangiocarcinoma and its correlation with nerve invasion and peripheral adipose tissue infiltration. Methods Paraffin sections from 156 patients with intrahepatic cholangiocarcinoma were collected from October 2013 to October 2020 in the Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital of Kunming Medical University, and the normal liver tissues from 30 cases of hepatic hemangioma undergoing partial hepatectomy were collected during the same period. In the control group, the immunohistochemical methods were used to detect the expression level of eIF5A2, and pathological data were collected to analyze the correlation between eIF5A2 and nerve invasion and peripheral adipose tissue infiltration. Results In 156 cases of intrahepatic cholangiocarcinoma tissue sections, 95 cases （60.9%） had high expression of eIF5A2. The high expression rate of eIF5A2 in normal liver tissue bile duct epithelium was 13.3%. The expression of eIF5A2 was significantly related to nerve invasion and peripheral adipose tissue infiltration （P < 0.05）. Conclusion eIF5A2 is highly expressed in intrahepatic cholangiocarcinoma and is significantly related to nerve invasion and surrounding adipose tissue infiltration.
- Intrahepatic cholangiocarcinoma /
- Eukaryotic translation initiation factor-5A2 /
- Immunohistochemical staining

HTML全文

图 1 eIF5A2在肝内胆管癌组织中的表达（×200）

A：肝内胆管癌组织HE染色；B：肝内胆管癌eIF5A2表达（+）；C：肝内胆管癌eIF5A2表达（++）；D：肝内胆管癌eIF5A2表达（+++）。

Figure 1. The expression of eIF5A2 in intrahepatic cholangiocarcinoma tissue（×200）

下载: 全尺寸图片幻灯片

图 2 eIF5A2在正常肝组织中的表达（×400）

A：正常肝组织eIF5A2表达（-）；B：正常肝组织eIF5A2表达（+）。

Figure 2. The expression of eIF5A2 in normal liver tissues（×400）

下载: 全尺寸图片幻灯片

表 1 eIF5A2在肝内胆管癌组织与正常肝组织表达的比较[n（%）]

Table 1. Comparison of eIF5A2 expression in intrahepatic cholangiocarcinoma tissues and normal liver tissues [n（%）]

项目	n	eIF5A2 高表达	eIF5A2 低表达	χ²	P
肝内胆管癌	156	95（60.9）	61（39.1）	22.865	< 0.001
正常肝组织	30	4（13.3）	26（86.7）	22.865	< 0.001

下载: 导出CSV

表 2 eIF5A2的表达与神经侵犯、周围脂肪组织浸润的相关性比较[n（%）]

Table 2. Correlation between the expression of eIF5A2 and nerve invasion and peripheral adipose tissue infiltration [n（%）]

项目	n（例）	神经侵犯n（%）	周围脂肪组织浸润n（%）
eIF5A2高表达	95	53（55.8）^*	30（31.6）^*
eIF5A2低表达	61	22（36.1）	9（14.8）
χ²		5.789	5.608
P		0.016	0.018
与eIF5A2低表达组相比，^*P < 0.05。

下载: 导出CSV

参考文献(27)

[1]	De Jong M C,Nathan H,Sotiropoulos G C,et al. Intrahepatic cholangiocarcinoma:An international multi-institutional analysis of prognostic factors and lymph node assessment[J]. J Clin Oncol,2011,29(23):3140-3145. doi: 10.1200/JCO.2011.35.6519
[2]	Guan X Y,Sham J S,Tang T C,et al. Isolation of a novel candidate oncogene within a frequently amplified region at 3q26 in ovarian cancer[J]. Cancer Res,2001,61(9):3806-3809.
[3]	Meng Q B,Peng J J,Qu Z W,et al. Eukaryotic initiation factor 5A2 and human digestive system neoplasms[J]. World J Gastrointest Oncol,2019,11(6):449-458. doi: 10.4251/wjgo.v11.i6.449
[4]	Bao Y,Lu Y L,Wang X,et al. Eukaryotic translation initiation factor 5A2 (eIF5A2) regulates chemoresistance in colorectal cancer through pithelial mesenchymal transition[J]. Cancer Cell Int,2015,15(2):1-7. doi: 10.1186/s12935-015-0250-9
[5]	Tang D J,Dong S S,Ma N F,et al. Overexpression of eukaryotic initiation factor 5A2 enhances cell motility and promotes tumor metastasis in hepatocellular carcinoma[J]. Hepatology,2010,51(4):1255-1263. doi: 10.1002/hep.23451
[6]	Tsang F H,Au V,Lu W J,et al. Prognostic marker microRNA-125b inhibits tumorigenic properties of hepatocellular carcinoma cells via suppressing tumorigenic molecule eIF5A2[J]. Dig Dis Sci,2014,59(10):2477-2487. doi: 10.1007/s10620-014-3184-5
[7]	Wu Y,Tang Y,Xie S,et al. Chimeric peptide supramolecular nanoparticles for plectin-1 targeted miRNA-9 delivery in pancreatic cancer[J]. Theranostics,2020,10(3):1151-1165. doi: 10.7150/thno.38327
[8]	Wei Y X,Chen G,You L,et al. Expression of eukaryotic translation initiation factor 5A2 in pancreatic adenocarcinoma and its correlation with the prognosis[J]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao,2013,35(6):634-638.
[9]	Meng Q B,Kang W M,Yu J C,et al. Overexpression of eukaryotic translation initiation factor 5A2 (EIF5A2) correlates with cell aggressiveness and poor survival in gastric cancer[J]. PLoS One,2015,10(3):e0119229. doi: 10.1371/journal.pone.0119229
[10]	Patel T. Increasing incidence and mortality of primary intrahepatic cholangiocarcinoma in the United States[J]. Hepatology,2001,33(6):1353-1357. doi: 10.1053/jhep.2001.25087
[11]	吴孟超, 吴在德. 黄家驷外科学[M]. 第7版. 北京: 人民卫生出版社, 2008: 1695.
[12]	Lu J,Zhao H W,Chen Y,et al. Eukaryotic translation initiation factor 5A2 is highly expressed in prostate cancer and predicts poor prognosis[J]. Exp Ther Med,2019,17(5):3741-3747.
[13]	Yang H,Li X D,Zhou Y,et al. Stemness and chemotherapeutic drug resistance induced by EIF5A2 overexpression in esophageal squamous cell carcinoma[J]. Oncotarget,2015,6(28):26079-26089. doi: 10.18632/oncotarget.4581
[14]	Chen W,Luo J H,Hua W F,et al. Overexpression of EIF-5A2 is an independent predictor of outcome in patients of urothelial carcinoma of the bladder treated with radical cystectomy[J]. Cancer Epidemiol Biomarkers Prev,2009,18(2):400-408. doi: 10.1158/1055-9965.EPI-08-0754
[15]	Wang F W,Guan X Y,Xie D. Roles of eukaryotic initiation factor 5A2 in human cancer[J]. Int J Biol Sci,2013,9(10):1013-1020. doi: 10.7150/ijbs.7191
[16]	韦颍昕,陈革,由磊,等. 真核翻译起始因子5A2在胰腺癌中的表达及与预后的相关性[J]. 中国医学科学院学报,2013,35(06):634-638. doi: 10.3881/j.issn.1000-503X.2013.06.009
[17]	孟庆彬. EIF5A2促进胃癌细胞增殖、侵袭的分子机制研究[D]. 北京: 北京协和医学院博士论文, 2013.
[18]	Marchet A,Mocellin S,Belluco C,et al. Gene expression profile of primary gastric cancer:Towards the prediction of lymph node status[J]. Ann Surg Oncol,2007,14(3):1058-1064. doi: 10.1245/s10434-006-9090-0
[19]	Xie D,Ma N F,Pan Z Z,et al. Overexpression of EIF-5A2 is associated with metastasis of human colorectal carcinoma[J]. Hum Pathol,2008,39(1):80-86. doi: 10.1016/j.humpath.2007.05.011
[20]	Liu R R,Lv Y S,Tang Y X,et al. Eukaryotic translation initiation factor 5A2 regulates the migration and invasion of hepatocellular carcinoma cells via pathways involving reactive oxygen species[J]. Oncotarget,2016,7(17):24348-24360. doi: 10.18632/oncotarget.8324
[21]	Fujimura K,Wright T,Strnadel J,et al. A hypusine-eIF5A-PEAK1 switch regulates the pathogenesis of pancreatic cancer[J]. Cancer Res,2014,74(22):6671-6681. doi: 10.1158/0008-5472.CAN-14-1031
[22]	Zhu W,Cai M Y,Tong Z T,et al. Overexpression of EIF5A2 promotes colorectal carcinoma cell aggressiveness by upregulating MTA1 through C-myc to induce epithelial-mesenchymaltransition[J]. Gut,2012,61(4):562-575. doi: 10.1136/gutjnl-2011-300207
[23]	Li Y,Fu L,Li J B,et al. Increased expression of EIF5A2,via hypoxia or gene amplification,contributes to metastasis and angiogenesis of esophageal squamous cell carcinoma[J]. Gastroenterology,2014,146(7):1701-1713. doi: 10.1053/j.gastro.2014.02.029
[24]	Luo J H,Hua W F,Rao H L,et al. Overexpression of EIF-5A2 predicts tumor recurrence and progression in pTa/pT1 urothelial carcinoma of the bladder[J]. Cancer Sci,2009,100(5):896-902. doi: 10.1111/j.1349-7006.2009.01126.x
[25]	Dong J S,Wu B,Zha Z L,et al. MicroRNA-588 regulates migration capacity and invasiveness of renal cancer cells by targeting EIF5A2[J]. Eur Rev Med Pharmacol Sci,2019,23(23):10248-10256.
[26]	Zhao K,Ye Z,Li Y,et al. LncRNA FTX contributes to the progression of colorectal cancer through regulating miR-192-5p/EIF5A2 axis[J]. Onco Targets Ther,2020,13(3):2677-2688.
[27]	Li X,He J,Ren X,Zhao H,et al. Circ_0003998 enhances doxorubicin resistance in hepatocellular carcinoma by regulating miR-218-5p/EIF5A2 pathway[J]. Diagn Pathol,2020,15(1):141-154. doi: 10.1186/s13000-020-01056-1

相关文章(20)

[1]	宋飞, 向盈盈, 车佳音, 李红阳, 徐文勇, 魏凌潇, 黄明. 胆道¹²⁵I 粒子支架与金属裸支架治疗Bismuth Corlette Ⅲ型胆管癌合并梗阻性黄疸的临床对比, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20221110
[2]	邹垚, 王梓瑜, 李皎, 李兴欣, 申亚琼, 徐敬, 金焰. LOXL2及Snail蛋白表达水平与胆管癌患者术后生存率的关系, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20221215
[3]	廖周俊, 杨少华, 刘立鑫, 胡晟, 陈轶晖, 康强, 张小文. AK4对肝内胆管癌细胞HUCCT1增殖、迁移的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220611
[4]	李刚, 杨少华, 沈甍, 谢楠, 张贯博, 王家兴, 李金淞. EIF5A2和Ki67在肝内胆管癌组织中的表达及临床意义, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220630
[5]	李留峥, 陆顺, 张世荣, 徐雷升, 赵海荣, 俸家伟, 高学昌, 吴彤. 联合半肝切除与围肝门切除治疗肝门部胆管癌的疗效, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20211014
[6]	李志强, 高红强, 李镜锋, 余杰琦, 王冬冬, 陈刚, 褚光. ICAM-1、MMP-9及NF-κB在胆管癌中的表达及与外周血免疫抑制细胞的关系, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20211117
[7]	李刚, 李金凇, 谢楠, 李春满, 魏雷, 曹凡, 苏莹珍. B7-H4在肝内胆管癌的表达及临床意义, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210415
[8]	侯峰强, 董山潮, 雷喜锋, 张伟. 紫杉醇对人胆管癌QBC939细胞wnt/β-catenin信号通路基因和蛋白表达的影响, 昆明医科大学学报.
[9]	康强. E-cadherin和Vimentin在肝内胆管癌的表达及临床意义, 昆明医科大学学报.
[10]	李政. 磁共振结合CA199、CEA、CA50、ALP对诊断肝内胆管结石合并早期胆管癌的可行性, 昆明医科大学学报.
[11]	王晔. 核结合因子-κB及bcl-2/bax在PQ中毒鼠肺组织中的表达, 昆明医科大学学报.
[12]	王天阳. 洛铂对人胆管癌RBE细胞凋亡显微结构及超微结构的作用观察, 昆明医科大学学报.
[13]	杨艳梅. 肝内胆管乳头状囊腺癌穿刺引流1例报道, 昆明医科大学学报.
[14]	潘宜双. 解剖性肝切除在治疗双侧肝内胆管结石中的临床应用价值, 昆明医科大学学报.
[15]	孙华. F-FDG PET/CT在肝内胆管细胞癌诊断中的应用, 昆明医科大学学报.
[16]	杨世昆. 肝右三叶切除并门静脉切除和重建治疗肝门胆管癌, 昆明医科大学学报.
[17]	. 左肝外叶切除治疗左肝内胆管结石79例疗效分析, 昆明医科大学学报.
[18]	黄松泉. 左肝外叶切除治疗左肝内胆管结石79例疗效分析, 昆明医科大学学报.
[19]	. 抗凋亡基因Survivin在分化型甲状腺癌中的表达及临床意义, 昆明医科大学学报.
[20]	宋宇锋. 去势大鼠关节软骨病理变化的研究, 昆明医科大学学报.