真核翻译起始因子-5A2在肝内胆管癌中的表达及意义

杨少华; 廖周俊; 胡晟; 洪茂林; 宋志美; 危群; 张小文

doi:10.12259/j.issn.2095-610X.S20210505

真核翻译起始因子-5A2在肝内胆管癌中的表达及意义

doi: 10.12259/j.issn.2095-610X.S20210505

1.
昆明医科大学第二附属医院肝胆胰外科二病区
2.
病理科，云南昆明　650101

基金项目: 国家自然科学基金资助项目（81760430）

详细信息

作者简介:
杨少华（1988～），男，陕西渭南人，在读博士研究生，主要从事肝胆胰外科疾病的临床与基础研究工作

通讯作者:
张小文，E-mail：zhangxiaowenlu@hotmail.com

中图分类号: R735.8
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出版历程
- 收稿日期: 2021-01-04
- 网络出版日期: 2021-06-02
- 刊出日期: 2021-05-20

The Expression and Significance of Eukaryotic Translation Initiation Factor-5A2 in Intrahepatic Cholangiocarcinoma

1.
Dept. of Hepatobiliary and Pancreatic Surgery
2.
Dept. of Pathology，The Second Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

摘要

摘要: 目的研究真核翻译起始因子-5A2（eukaryotic translation initiation factor 5A2，eIF5A2）在肝内胆管癌中的表达及与神经侵犯和周围脂肪组织浸润的相关性。方法收集昆明医科大学第二附属医院肝胆胰外科2013年10月至2020年10月156例肝内胆管癌患者术后石蜡切块，收集同期30例肝血管瘤患者部分肝切除的正常肝组织作为对照组，采用免疫组化的方法检测eIF5A2的表达水平，并收集病理资料，分析eIF5A2与神经侵犯和周围脂肪组织浸润的相关性。结果 156例肝内胆管癌组织切片中eIF5A2高表达95例（60.9%），其在正常肝组织胆管上皮高表达率为13.3%，eIF5A2的表达与神经侵犯和周围脂肪组织浸润显著相关（P < 0.05）。结论 eIF5A2在肝内胆管癌中高表达，与神经侵犯和周围脂肪组织浸润显著相关。
- 肝内胆管癌 /
- 真核翻译起始因子-5A2 /
- 免疫组织化学染色
Abstract: Objective To study the expression of eukaryotic translation initiation factor 5A2 （eIF5A2） in intrahepatic cholangiocarcinoma and its correlation with nerve invasion and peripheral adipose tissue infiltration. Methods Paraffin sections from 156 patients with intrahepatic cholangiocarcinoma were collected from October 2013 to October 2020 in the Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital of Kunming Medical University, and the normal liver tissues from 30 cases of hepatic hemangioma undergoing partial hepatectomy were collected during the same period. In the control group, the immunohistochemical methods were used to detect the expression level of eIF5A2, and pathological data were collected to analyze the correlation between eIF5A2 and nerve invasion and peripheral adipose tissue infiltration. Results In 156 cases of intrahepatic cholangiocarcinoma tissue sections, 95 cases （60.9%） had high expression of eIF5A2. The high expression rate of eIF5A2 in normal liver tissue bile duct epithelium was 13.3%. The expression of eIF5A2 was significantly related to nerve invasion and peripheral adipose tissue infiltration （P < 0.05）. Conclusion eIF5A2 is highly expressed in intrahepatic cholangiocarcinoma and is significantly related to nerve invasion and surrounding adipose tissue infiltration.
- Intrahepatic cholangiocarcinoma /
- Eukaryotic translation initiation factor-5A2 /
- Immunohistochemical staining

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图 1 eIF5A2在肝内胆管癌组织中的表达（×200）

A：肝内胆管癌组织HE染色；B：肝内胆管癌eIF5A2表达（+）；C：肝内胆管癌eIF5A2表达（++）；D：肝内胆管癌eIF5A2表达（+++）。

Figure 1. The expression of eIF5A2 in intrahepatic cholangiocarcinoma tissue（×200）

下载: 全尺寸图片幻灯片

图 2 eIF5A2在正常肝组织中的表达（×400）

A：正常肝组织eIF5A2表达（-）；B：正常肝组织eIF5A2表达（+）。

Figure 2. The expression of eIF5A2 in normal liver tissues（×400）

下载: 全尺寸图片幻灯片

表 1 eIF5A2在肝内胆管癌组织与正常肝组织表达的比较[n（%）]

Table 1. Comparison of eIF5A2 expression in intrahepatic cholangiocarcinoma tissues and normal liver tissues [n（%）]

项目	n	eIF5A2 高表达	eIF5A2 低表达	χ²	P
肝内胆管癌	156	95（60.9）	61（39.1）	22.865	< 0.001
正常肝组织	30	4（13.3）	26（86.7）	22.865	< 0.001

下载: 导出CSV

表 2 eIF5A2的表达与神经侵犯、周围脂肪组织浸润的相关性比较[n（%）]

Table 2. Correlation between the expression of eIF5A2 and nerve invasion and peripheral adipose tissue infiltration [n（%）]

项目	n（例）	神经侵犯n（%）	周围脂肪组织浸润n（%）
eIF5A2高表达	95	53（55.8）^*	30（31.6）^*
eIF5A2低表达	61	22（36.1）	9（14.8）
χ²		5.789	5.608
P		0.016	0.018
与eIF5A2低表达组相比，^*P < 0.05。

下载: 导出CSV

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