恶性梗阻性黄疸患者与健康人粪菌群的对比

孙岩波; 李为明; 李树民; 林岳瀛; 岑云云; 徐鹏远

doi:10.12259/j.issn.2095-610X.S20210516

恶性梗阻性黄疸患者与健康人粪菌群的对比

doi: 10.12259/j.issn.2095-610X.S20210516

孙岩波^{1, 2},
李为明^{1, 2},
李树民^{1, 2},
林岳瀛^{1, 2},
岑云云^{1, 2, ,},
徐鹏远^{1, 2}

1.
昆明医科大学第二附属医院胃肠外科，云南昆明　650101
2.
云南省外科临床营养研究中心，云南昆明　650101

基金项目: 云南省外科临床营养研究中心基金资助项目（2018NS0317，2018NS0318）；云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2019FE001（-223）]；云南省卫生健康委员会医学后备人才培养计划基金资助项目（H-2018067）

详细信息

作者简介:
孙岩波（1982～），男，山东滨州人，医学硕士，主治医师，主要从事普外科及临床营养临床及科研工作。李为明与孙岩波对本文有同等贡献

通讯作者:
岑云云，E-mail：glwk0981@sina.com

中图分类号: R459.3
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出版历程
- 收稿日期: 2021-01-08
- 网络出版日期: 2021-06-03
- 刊出日期: 2021-05-20

A Comparative Study of Fecal Bacteria between Patients with Malignant Obstructive Jaundice and Healthy People

Yan-bo SUN^{1, 2},
Wei-ming LI^{1, 2},
Shu-min LI^{1, 2},
Yue-ying LIN^{1, 2},
Yun-yun CEN^{1, 2
, ,},
Peng-yuan XU^{1, 2}

1.
Dept. of Gastrointestinal Surgery，The 2nd Affiliated Hospital of Kunming Medical University. Kunming Yunnan 650101
2.
Research Center for Surgical Clinical Nutrition of Yunnan Province，Kunming Yunnan 650101，China

摘要

摘要: 目的初步探索恶性梗阻性黄疸患者粪菌群变化特征。方法以2019年1月至2019年5月入住昆明医科大学第二附属医院的87例恶行梗阻性黄疸患者为病例库，经纳入排除标准筛选，最终选取10例患者为研究对象、10例患者健康亲属作为对照组标本采集对象，记录年龄、性别、BMI；送检粪便测粪便中菌群16srDNA拷贝数。结果（1）实验组粪菌群丰度和多样性指数比对照组低，但无统计学意义（P > 0.05）；但是将丰度数据以群落Heatmap图呈现，直观差异明显；（2）将两组数据以菌属为单位按比例细分，发现实验组粪菌属组成较对照组发生了根本性的变化（P < 0.05），其中大肠杆菌志贺菌、链球菌、韦荣氏球菌属、肠球菌属、拟杆菌属显著增加，而魏斯式菌属、梭状芽胞杆菌属等菌属大幅下降。结论恶性梗阻性黄疸患者患者粪菌群结构与正常对照组的粪便菌群结构之间存在明显差异，优势菌群菌属发生了根本性的改变。
- 恶性梗阻性黄疸 /
- 粪菌群 /
- 平衡
Abstract: Objective To explore the changing characteristics of fecal bacteria in patients with malignant obstructive jaundice. Methods From January 2019 to May 2019, 87 cases with malignant obstructive jaundice were taken as case bank and screened by inclusion and exclusion criteria. After that, 10 patients and 10 healthy relatives of patients were selected, age, sex and BMI were recorded, and faeces were sent for 16srDNA copy number. Results （1）The fecal bacteria abundance and diversity index of the experimental group were lower than that of the control group, but there was no statistical significance （P < 0.05）. However, when the data of fecal bacteria abundance were presented in the Heatmap, the intuitive difference was more obvious. （2） The data of the two groups were subdivided by bacteria genus, and it was found that the composition of fecal bacteria genus in the experimental group changed fundamentally compared with that in the control group （P < 0.05）. Among them, Escherichia-Shigella, Streptococcus, Veillonella, Enterococcus, Akkermansia increased significantly, while the bacteria genera of wiesia and clostridium declined significantly. Whether the intestinal flora can be rebalanced may affect the therapeutic effect. Conclusion The fecal flora structure of patients with malignant obstructive jaundice is significantly different from that of normal control group, and the dominant flora genus has been fundamentally changed.
- Malignant obstructive jaundice /
- Fecal bacteria /
- Balance

HTML全文

图 1 两组粪菌群丰度泛物种分析（pan物种）

Figure 1. Pan species analysis of fecal flora abundance in two groups （pan species）

下载: 全尺寸图片幻灯片

图 2 两组粪菌群丰度泛物种分析（Heatmap图）

Figure 2. Pan-species analysis of fecal flora abundance in two groups （Heatmap）

下载: 全尺寸图片幻灯片

图 3 两组间粪菌群多样性分析（sobs指数）

Figure 3. Analysis of faecal flora diversity between the two groups （sobs index）

下载: 全尺寸图片幻灯片

图 4 实验组粪菌群优势菌属比例图

Figure 4. Proportion diagram of dominant faecal flora in the experimental group

下载: 全尺寸图片幻灯片

表 1 患者一般临床资料比较（n/$\bar x \pm s$）

Table 1. Comparison of patients’ general clinical data （n/$\bar x \pm s$）

组别	n	性别（n）		年龄（岁）	BMI（kg/m²）
组别	n	男	女	年龄（岁）	BMI（kg/m²）
g1	10	6	4	57.00 ± 10.10	25.47 ± 2.60
g2	10	6	4	56.80 ± 7.62	26.38 ± 2.26
t				0.050	−0.843
P				P > 0.05	P > 0.05

下载: 导出CSV

表 2 两组间总体粪菌群丰度、多样性的比较

Table 2. Comparison of the abundance and diversity of total fecal flora between the two groups

组别	菌群丰度（pan物种）（$ \bar {{x}} \pm s$）	菌群多样性（sobs指数）（$ \bar {{x}} \pm s$）
g1	373.05 ± 104.01	165.00 ± 41.03
g2	402.01 ± 107.48	195.44 ± 40.54
t	−0.597	−1.624
P	P > 0.05	P > 0.05

下载: 导出CSV

表 3 两组优势菌属对比（$ \bar x \pm s$）

Table 3. Comparison of dominant bacteria genera between the two groups （$\bar x \pm s $）

组别	g1优势菌属	g2优势菌属
g1	12.53 ± 5.43	165.00 ± 41.03
g2	2.36 ± 2.07	0.74 ± 1.63
t	4.644	4.94
P	P < 0.05	P < 0.05

下载: 导出CSV

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