Determination of Volatile Components of Blaps rhynchoptera by Headspace Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry
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摘要:
目的 对喙尾琵甲干燥虫体的挥发性化学成分进行分析。 方法 采用顶空固相微萃取方法，结合气质联用技术对其中挥发性成分进行分析和鉴定，通过峰面积归一化法测定各成分的相对含量。 结果 经质谱扫描结合标准谱库检索对比，共鉴定出其中40个成分，占总峰面积的92.80%。其中烃类16种，占总含量的35.73%，以直链和支链烷烃为主；脂肪酸类8种，占总含量的35.87%，以短链脂肪酸为主；此外，还含有胺类、醇类、酯类、醛类、酚类、酮类、醚类等成分。含量在5%以上的组分有十三烷（21.47%）、3-甲基丁酸（16.88%）、2-甲基丁酸（7.67%）、三甲胺（6.59%）和乙酸（5.47%）。 结论 喙尾琵甲干燥虫体中的挥发性成分以烃类和短链脂肪酸类化合物为主，这些成分具有多种生理活性，为药用昆虫资源的进一步开发利用提供参考。 Abstract:Objective To determine the volatile components of the dried Blaps rhynchoptera Fairmaire. Methods Headspace solid phase microextraction （HS-SPME） combined with gas chromatography-mass spectrometry （GC-MS） were used to analysis and identify the volatile components, and the relative content of each compound was calculated using the area normalization method. Results Through mass spectrum scanning combined with mass standard library, 40 components were identified, accounting for 92.80% of the total peak area. Sixteen hydrocarbons were represented by straight-chain and branched alkanes, accounting for 35.73%. Eight fatty acids were represented by short-chain fatty acids, accounted for 35.87%. In addition, it also contained amines, alcohols, esters, aldehydes, phenols, ketones, and ethers. Tridecane, 3-methylbutyric acid, 2-methylbutyric acid, trimethylamine and acetic acid were identified as the major components, accounting for 21.47%, 16.88%, 7.67%, 6.59% and 5.47%, respectively. Conclusion Hydrocarbons and short-chain fatty acids are main volatile components in the dried B. rhynchoptera, which have a variety of pharmacological activities. The results could provide a theoretical basis for the further development and utilization of the medicinal insect resources. -
图 1 喙尾琵甲挥发性成分总离子流图
Figure 1. GC-MS ion flow map of volatile components from Blaps rhynchoptera
表 1 喙尾琵甲挥发性成分分析结果
Table 1. Volatile components from Blaps rhynchoptera
序号 保留时间（min） 化合物名称 分子式 分子量 含量（%） 1 1.16 三甲胺 N，N-Dimethylmethylamine C3H9N 59.11 6.59 2 1.67 乙酸 Acetic acid C2H4O2 60.05 5.47 3 2.15 异戊醛 3-Methylbutanal C5H10O 86.13 1.25 4 2.63 丙酸 Propanoic acid C3H6O2 74.08 0.96 5 3.92 异丁酸 Isobutyric acid C4H8O2 88.11 2.92 6 4.11 2，3-丁二醇 2，3-Butanediol C4H10O2 90.12 1.34 7 4.32 1，3-丁二醇 1，3-Butanediol C4H10O2 90.12 2.99 8 6.06 异戊酸乙酯 Ethyl isopentanoate C7H14O2 130.18 2.69 9 6.81 3-甲基丁酸 Isovaleric acid C5H10O2 102.13 16.88 10 7.17 2-甲基丁酸 2-Methyl butyric acid C5H10O2 102.13 7.67 11 9.10 二乙二醇单甲醚 Ethanol，2-（2-methoxyethoxy）- C5H12O3 120.15 0.33 12 10.22 4-甲基戊酸 4-Methylvaleric acid C6H12O2 116.16 1.10 13 10.32 4-甲基戊酸乙酯 Ethyl 4-methylvalerate C8H16O2 144.21 0.51 14 11.30 苯酚 Phenol C6H6O 94.11 0.54 15 11.39 1，2，4-三甲基苯 1，2，4-trimethyl-benzene C9H12 120.19 0.62 16 11.52 己酸 Hexanoic acid C6H12O2 116.16 0.37 17 13.04 N-（2-甲基丙基）乙酰胺 N-（2-Methylpropyl）acetamide C6H13NO 115.17 0.38 18 13.38 泛酰内酯 Pantolactone C6H10O3 130.14 0.29 19 14.15 γ-己内酯 4-Hexalactone C6H10O2 114.14 0.38 20 14.95 2-吡咯烷酮 2-Pyrrolidinone C4H7NO 85.10 0.32 21 16.09 芳樟醇 Linalool C10H18O 154.25 0.47 22 16.25 壬醛 Nonanal C9H18O 142.24 1.58 23 18.66 3，5，5-三甲基环己烯 3，5，5-Trimethy-cyclohexene C9H16 124.22 1.13 24 19.03 萘 Naphthalene C10H8 128.17 0.29 25 19.37 α-松油醇α-Terpineol C10H18O 154.25 0.74 26 19.67 十二烷 Dodecane C12H26 170.33 0.54 27 22.11 壬酸 Nonanoic acid C9H18O2 158.24 0.50 28 22.54 1-十三烯 1-Tridecene C13H26 182.34 3.55 29 22.8 十三烷 Tridecane C13H28 184.36 21.47 30 25.56 十四烷 Tetradecane C14H30 198.39 0.59 31 27.18 2，6，10-三甲基十二烷 2，6，10-Trimethyldodecane C15H32 212.41 0.78 32 27.49 2，6-二（叔丁基）-4-羟基-4-甲基-2，5-环己二烯-1-酮
2，6-Di（tert-butyl）-4-hydroxy-4-methyl-2，5-cyclohexadien-1-oneC15H24O2 236.35 0.80 33 27.94 1-十五烯 1-Pentadecene C15H30 210.40 0.83 34 28.14 十五烷 Pentadecane C15H32 212.41 2.60 35 28.37 α-法尼烯 α-Farnesene C15H24 204.35 0.45 36 30.52 十六烷 Hexadecane C16H34 226.44 1.37 37 31.65 2，6，10-三甲基十五烷2，6，10-Trimethylpentadecane C18H38 254.49 0.35 38 32.78 十七烷 Heptadecane C17H36 240.47 0.41 39 32.91 姥鲛烷 pentadecane，2，6，10，14-tetramethyl- C19H40 268.52 0.40 40 35.12 植烷 Hexadecane，2，6，10，14-tetramethyl- C20H42 282.55 0.35 
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