Expression and Significance of microRNA-21 and EGFR in Non-small Cell Lung Cancer
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摘要:
目的 研究microRNA-21及其调控基因EGFR在非小细胞肺癌-肺腺癌和鳞癌中的表达及意义。 方法 以 2020年 10月至 2020 年 12 月于昆明医科大学第一附属医院就诊的30例经病理确诊的非小细胞肺癌患者的肺组织标本作为研究对象，包括肺腺癌24例和肺鳞癌6例，同时以癌旁肺组织作为对照。实时定量PCR检测microRNA-21的表达，免疫组织化学方法检测microRNA-21调控基因EGFR的蛋白表达和定位。 结果 癌旁肺组织中microRNA-21和EGFR表达水平较低，与癌旁组织比较，肺腺癌和肺鳞癌组织中microRNA-21和EGFR表达均明显上调，差异有统计学意义（P < 0.01）；与肺腺癌组织比较，肺鳞癌组织中的microRNA-21和EGFR表达明显升高，差异有统计学意义（P < 0.05）。 结论 microRNA-21及其调控基因EGFR在非小细胞肺癌-肺腺癌和鳞癌中呈高表达，且表达趋势一致，可预测非小细胞肺癌的进展与预后。 -
关键词:
- 非小细胞肺癌 /
- microRNA-21 /
- 肺腺癌 /
- 肺鳞癌 /
- EGFR
Abstract:Objective To explore the expression and significance of microRNA-21 and its regulatory gene EGFR in non-small cell lung cancer, lung adenocarcinoma and squamous cell carcinoma. Methods Lung tissue samples of 30 patients with pathologically confirmed non-small cell lung cancer from October 2020 to December 2020 in The 1st Affiliated Hospital of Kunming Medical University were taken as the research objects, including 24 cases of lung adenocarcinoma and 6 cases of lung squamous cell carcinoma, and the lung tissue adjacent to cancer was taken as the control. The expression of microRNA-21 was detected by real-time quantitative PCR, and the protein expression and localization of EGFR were detected by immunohistochemistry. Results The expression levels of microRNA-21 and EGFR in lung tissue adjacent to cancer were lower. Compared with lung tissue adjacent to cancer, the expression levels of microRNA-21 and EGFR in lung adenocarcinoma and lung squamous cell carcinoma were significantly increased （P < 0.01）; compared with lung adenocarcinoma, the expression levels of microRNA-21 and EGFR in lung squamous cell carcinoma were significantly increased （P < 0.05）. Conclusion MicroRNA-21 and its regulatory gene EGFR are highly expressed in NSCLC lung adenocarcinoma and squamous cell carcinoma, and the expression trend is consistent, which can be used to predict the progress and prognosis of NSCLC. -
Key words:
- Non small cell lung cancer /
- MicroRNA-21 /
- Lung adenocarcinoma /
- Lung squamous cell carcinoma /
- EGFR
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图 1 典型非小细胞癌-肺腺癌与鳞癌的病理特征（HE染色，200 ×）
A：癌旁肺组织；B：肺腺癌；C肺鳞癌。
Figure 1. Pathological features of lung adenocarcinoma and squamous cell carcinoma （HE staining，200 ×）
图 2 典型非小细胞癌-肺腺癌与鳞癌的特征性标志物表达（免疫组化 200 ×）
A：癌旁肺组织TTF-1；B：肺腺癌TTF-1；C肺鳞癌P40。
Figure 2. Expression of characteristic markers in typical non-small cell carcinoma lung adenocarcinoma and squamous cell carcinoma （Immunohistochemical staining 200 ×）
图 3 非小细胞癌-肺腺癌与鳞癌中microRNA-21表达（
$\bar x \pm s $ ）与癌旁肺组织比较，**P < 0.01；与肺腺癌比较，#P < 0.05。
Figure 3. Expression of microRNA-21 in non-small cell carcinoma-lung adenocarcinoma and squamous cell carcinoma（
$\bar x \pm s $ ）
图 4 非小细胞癌-肺腺癌与鳞癌中EGFR蛋白表达（免疫组化，200 ×）
A：癌旁肺组织EGFR；B：肺腺癌EGFR；C肺鳞癌EGFR。
Figure 4. Expression of EGFR protein in non-small cell carcinoma-lung adenocarcinoma and squamous cell carcinoma （immunohistochemistry，200 ×）
图 5 非小细胞癌-肺腺癌与鳞癌中EGFR蛋白表达评分（
$\bar x \pm s $ ）与癌旁肺组织比较，**P < 0.01；与肺腺癌比较，#P < 0.05。
Figure 5. EGFR protein expression score in non-small cell carcinoma lung adenocarcinoma and squamous cell carcinoma（
$\bar x \pm s $ ）表 1 microRNA-21和内参U6的引物序列
Table 1. Primer sequences of microRNA-21 and internal reference U6
基因 引物序列 miR-21 F：5′-ACGTTGTGTAGCTTATCAGACTG-3′ R：5′-AATGGTTGTTCTCCACACTCTC-3′ 内参U6 F：5′-ATTGGAACGATACAGAGAAGATT-3′ R：5′-GGAACGCTTCACGAATTTG-3′ 
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