Tiger17 Promotes the Proliferation and Migration of Human Oral Mucosa Fibroblast Cells
-
摘要:
目的 观察在不同浓度 Tiger17作用于人口腔黏膜成纤维细胞（hOMF）不同时间后对细胞增殖和迁移的影响。 方法 培养hOMF细胞，CCK8和划痕实验检测不同浓度Tiger17作用于hOMF细胞不同时间后细胞增殖和迁移的变化；Western Blot 检测hOMF细胞表达TGF-β1、Erk1/2和p-Erk1/2蛋白的水平。 结果 100 μg/mL和200 μg/mL Tiger17对hOMF的增殖活性在48 h明显高于对照组（P < 0.05）；100 μg/mL Tiger17对hOMF的迁移率在24 h和48 h均明显高于对照组（P < 0.01）；不同浓度的Tiger17作用于hOMF 24 h和48 h后，细胞表达TGF-β1、p-Erk1/2蛋白升高，差异有统计学意义（P < 0.05）。 结论 Tiger17使hOMF细胞表达TGF-β1、p-Erk1/2升高，促进hOMF细胞的增殖和迁移。 Abstract:Objective To investigate the effects of different concentrations of Tiger17 on the proliferation and migration of human oral mucosa fibroblast cells （hOMF） for different periods of time. Methods The cells were cultured and migration of hOMF cells treated with different concentrations of Tiger17 for different time were detected by CCK8 and scratch test. The expression levels of TGF-β1, ERK1/2 and p-ERK1/2 in HOMF cells were detected by Western Blot. Results The proliferative activity of hOMF was significantly higher than that of the control group at 48 h （P < 0.05） in 100 μg/mL and 200 μg/mL Tiger17; the migration rate of hOMF was significantly higher than that of the control group at 24 h and 48 h （P < 0.01） in 100 μg/mL Tiger17; different concentrations of Tiger17 acted on hOMF for 24 h and 48 h, the expression of TGF-β1 and p-Erk1/2 protein increased, and the difference was statistically significant （P < 0.05）. Conclusion Tiger17 increases the expression of TGF-β1 and p-Erk1/2 in hOMF cells, and promotes the proliferation and migration of hOMF cells. -
Key words:
- Human oral mucosa fibroblast cells /
- Tiger17 /
- Promote /
- Cell proliferation /
- Cell migration
-
图 1 Tiger17促进hOMF细胞的增殖、迁移
A：不同浓度Tiger17影响细胞增殖；B：100 μg/mL Tiger17促进细胞迁移；C：100 μg/mL Tiger17促进细胞迁移统计图。与Control（对照组）比较，*P < 0.05，**P < 0.01。
Figure 1. Tiger17 promotes the proliferation and migration of hOMF cells
图 2 Tiger17促进hOMF细胞TGF-β1、p-Erk1/2表达的情况
A：不同浓度Tiger17作用细胞24 h和48 h后TGF-β1、Erk1/2、p-Erk1/2蛋白表达情况；B：不同浓度Tiger17作用细胞24 h和48 h后TGF-β1蛋白相对表达量；C：不同浓度Tiger17作用细胞24 h和48 h后p-Erk1/2蛋白相对表达量。与0 μg/mL （对照组）比较，*P < 0.05。
Figure 2. Tiger17 promotes the expression of TGF-β1 and p-Erk1/2 in hOMF cells
-
[1] 许竞,崔宝仪,高文峰,等. 即刻种植牙龈软组织伤口的特点及其愈合方式[J]. 广东医学,2013,34(7):1066-1069. doi: 10.3969/j.issn.1001-9448.2013.07.031 [2] Liu H,Duan Z,Tang J,et al. A short peptide. from frog skin accelerates diabetic wound healing[J]. FEBS J,2014,281(20):4633-4643. doi: 10.1111/febs.12968 [3] Tang J,Liu H,Gao C,et al. A small peptide with potential ability to promote wound healing[J]. PLoS One,2014,9(3):e92082. doi: 10.1371/journal.pone.0092082 [4] Liu H,Mu L,Tang J,et al. A potential wound healing-promoting peptide from frog skin[J]. Int J Biochem Cell Biol,2014,49(2):32-41. [5] Mu L,Tang J,Liu H,et al. A potential wound-healing-promoting peptide from salamander skin[J]. FASEB J,2014,28(9):3919-3929. doi: 10.1096/fj.13-248476 [6] Miyazaki A,Ohkubo T,Hatta M,et al. Integrin alpha6 beta4 and TRPV1 channel coordi-nately regulate directional keratinocyt emigration[J]. Biochem BiophysRes commun,2015,458(1):161-167. doi: 10.1016/j.bbrc.2015.01.086 [7] Mirza R,DiPietro L A,Koh T J. selective and specific macrophage ablation is detrimental to wound healing in mice[J]. Am J Pathol,2009,175(6):2454-2462. doi: 10.2353/ajpath.2009.090248 [8] Lucas T,Waisman A,Ranjan R,et al. differential roles of macrophages in diverse phases of skin repair[J]. J Immunol,2010,184(7):3964-3977. doi: 10.4049/jimmunol.0903356 [9] Daley J M,Brancato S K,Thomay A A,et al. The phenotype of murine wound macrophages[J]. J Leukoc Biol,2010,87(1):59-67. doi: 10.1189/jlb.0409236 [10] Wang Y,Yang L,Yang Y,et al. Liver kinase B1 (LKB1) regulates proliferation and apoptosis of non-small cell lung cancer A549 cells via targeting ERK signaling pathway[J]. Cancer Management and Research,2021,39(13):65-74. [11] Qian L L,Ji J J,Guo J Q,et al. Protective role of serpina3c as a novel thrombin inhibitor against atherosclerosis in mice[J]. Clin Sci (Lond),2021,135(3):447-463. doi: 10.1042/CS20201235 -
下载:
点击查看大图
图(2)
计量
- 文章访问数: 4179
- HTML全文浏览量: 2226
- PDF下载量: 31
- 被引次数: 0
