The GalR2 Mediated Analgesic Effect of Needle Knife in Nucleus Accumbens of Rats with Neuropathic Pain
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摘要:
目的 研究伏核（nucleus accumbens，NAc）神经细胞甘丙肽受体2（galanin receptor 2，GalR2）激活介导针刀干预神经痛的作用机制。 方法 采用左侧坐骨神经部分结扎（chronic constriction injury，CCI）制作神经痛动物模型，通过测定大鼠对伤害性热刺激诱导的后爪缩爪潜伏期（hind paw withdrawal latency，HWL）和机械刺激诱导的后爪缩爪阈值（hind paw withdrawal threshold，HWT）来检测痛觉阈值。Western blot法检测针刀治疗对CCI大鼠伏核神经细胞GalR2表达的影响，并于CCI大鼠伏核内微量注射甘丙肽受体的阻断剂后，检测其对针刀干预神经痛的痛觉阈值的影响。 结果 CCI大鼠伏核内注射2 nmoL甘丙肽受体的非特异性阻断剂Galantide减弱了针刀疗法引起的HWL（左侧：P < 0.001；右侧：P < 0.05）和HWT（左侧：P < 0.01；右侧：P < 0.05）的延长，但注射1 nmoL的Galantide没有显著性差异（P > 0.05）。针刀治疗引起CCI大鼠伏核神经细胞GalR2的表达上调（P < 0.01），且注射2 nmoL的GalR2的特异性阻断剂M871后削弱了针刀疗法引起的HWL（左侧：P < 0.001；右侧：P < 0.001）和HWT（左侧：P < 0.001；右侧：P < 0.01）。 结论 CCI大鼠伏核神经细胞GalR2激活可能介导了针刀干预神经痛的镇痛作用。 Abstract:Objective To investigate the mechanism of galanin receptor 2 （GalR2） in nucleus accumbens （NAc） mediated needle knife intervention in neuralgia. Methods A mononeuropathy model was duplicated by chronic constriction injury （CCI） of the left sciatic nerve of rat. The hind paw withdraw latency （HWL） to noxious thermal stimulation and the hind paw withdraw threshold （HWT） to noxious mechanical stimulation were tested. Western blot was used to detect the effect of needle knife therapy on the expression of GalR2 in NAc of CCI rats. Finally, the HWL and HWT were tested by intra-NAc injection of galanin receptor blockers after the needle knife therapy in CCI rats. Results The increases of HWL （left: P < 0.001; right: P < 0.05）and HWT （left: P < 0.01; right: P < 0.05）induced by the needle knife therapy were reduced by intra-NAc injection of 2 nmol galanin receptors blocker galantide in CCI rats, but not 1 nmol galantide （P > 0.05）. The expression of GalR2 in NAc of CCI rats was upregulated by the needle knife therapy （P < 0.01）, and intra-NAc injections of 2 nmol of GalR2 specific blocker M871 weakened the increase of HWL （left: P < 0.001; right: P < 0.001）and HWT （left: P < 0.001; right: P < 0.01）induced by the needle knife therapy in CCI rats. Conclusion GalR2 may mediate the analgesic effect of the needle knife therapy in NAc of CCI rats. -
Key words:
- GalR2 /
- Needle knife /
- Neuropathic pain /
- Analgesic effect
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图 2 CCI大鼠伏核内注射galantide对针刀镇痛作用的影响
A：左侧HWL；B：右侧HWL；C：左侧HWT；D：右侧HWT。与“针刀治疗+生理盐水”对照组比较，*P < 0.05、**P < 0.01、***P < 0.00；不同组之间同一时间点的比较，#P < 0.05、###P < 0.001。
Figure 2. Effects of intra-NAc injection of galantide on the analgesia of needle knife in CCI rats
图 3 针刀干预对CCI大鼠伏核神经细胞GalR2表达的影响
A：Western blot 检测GalR2和GAPDH的表达条带；B：GalR2/GAPDH的比值图。与对照组和模型组比较， **P < 0.01。
Figure 3. Effect of needle knife therapy on GalR2 expression in NAc of CCI rats
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