The GalR2 Mediated Analgesic Effect of Needle Knife in Nucleus Accumbens of Rats with Neuropathic Pain
-
摘要:
目的 研究伏核（nucleus accumbens，NAc）神经细胞甘丙肽受体2（galanin receptor 2，GalR2）激活介导针刀干预神经痛的作用机制。 方法 采用左侧坐骨神经部分结扎（chronic constriction injury，CCI）制作神经痛动物模型，通过测定大鼠对伤害性热刺激诱导的后爪缩爪潜伏期（hind paw withdrawal latency，HWL）和机械刺激诱导的后爪缩爪阈值（hind paw withdrawal threshold，HWT）来检测痛觉阈值。Western blot法检测针刀治疗对CCI大鼠伏核神经细胞GalR2表达的影响，并于CCI大鼠伏核内微量注射甘丙肽受体的阻断剂后，检测其对针刀干预神经痛的痛觉阈值的影响。 结果 CCI大鼠伏核内注射2 nmoL甘丙肽受体的非特异性阻断剂Galantide减弱了针刀疗法引起的HWL（左侧：P < 0.001；右侧：P < 0.05）和HWT（左侧：P < 0.01；右侧：P < 0.05）的延长，但注射1 nmoL的Galantide没有显著性差异（P > 0.05）。针刀治疗引起CCI大鼠伏核神经细胞GalR2的表达上调（P < 0.01），且注射2 nmoL的GalR2的特异性阻断剂M871后削弱了针刀疗法引起的HWL（左侧：P < 0.001；右侧：P < 0.001）和HWT（左侧：P < 0.001；右侧：P < 0.01）。 结论 CCI大鼠伏核神经细胞GalR2激活可能介导了针刀干预神经痛的镇痛作用。 Abstract:Objective To investigate the mechanism of galanin receptor 2 （GalR2） in nucleus accumbens （NAc） mediated needle knife intervention in neuralgia. Methods A mononeuropathy model was duplicated by chronic constriction injury （CCI） of the left sciatic nerve of rat. The hind paw withdraw latency （HWL） to noxious thermal stimulation and the hind paw withdraw threshold （HWT） to noxious mechanical stimulation were tested. Western blot was used to detect the effect of needle knife therapy on the expression of GalR2 in NAc of CCI rats. Finally, the HWL and HWT were tested by intra-NAc injection of galanin receptor blockers after the needle knife therapy in CCI rats. Results The increases of HWL （left: P < 0.001; right: P < 0.05）and HWT （left: P < 0.01; right: P < 0.05）induced by the needle knife therapy were reduced by intra-NAc injection of 2 nmol galanin receptors blocker galantide in CCI rats, but not 1 nmol galantide （P > 0.05）. The expression of GalR2 in NAc of CCI rats was upregulated by the needle knife therapy （P < 0.01）, and intra-NAc injections of 2 nmol of GalR2 specific blocker M871 weakened the increase of HWL （left: P < 0.001; right: P < 0.001）and HWT （left: P < 0.001; right: P < 0.01）induced by the needle knife therapy in CCI rats. Conclusion GalR2 may mediate the analgesic effect of the needle knife therapy in NAc of CCI rats. -
Key words:
- GalR2 /
- Needle knife /
- Neuropathic pain /
- Analgesic effect
-
图 2 CCI大鼠伏核内注射galantide对针刀镇痛作用的影响
A：左侧HWL；B：右侧HWL；C：左侧HWT；D：右侧HWT。与“针刀治疗+生理盐水”对照组比较，*P < 0.05、**P < 0.01、***P < 0.00；不同组之间同一时间点的比较，#P < 0.05、###P < 0.001。
Figure 2. Effects of intra-NAc injection of galantide on the analgesia of needle knife in CCI rats
图 3 针刀干预对CCI大鼠伏核神经细胞GalR2表达的影响
A：Western blot 检测GalR2和GAPDH的表达条带；B：GalR2/GAPDH的比值图。与对照组和模型组比较， **P < 0.01。
Figure 3. Effect of needle knife therapy on GalR2 expression in NAc of CCI rats
-
[1] Jensen T S,Baron R,Haanpää M,et al. A new definition of neuropathic pain[J]. Pain,2011,152(10):2204-2205. doi: 10.1016/j.pain.2011.06.017 [2] 王燮荣. 针刀医学简论[J]. 中国中医药现代远程教育,2010,8(10):39. doi: 10.3969/j.issn.1672-2779.2010.10.034 [3] 赵明雷,白跃宏,张颖,等. 小针刀治疗膝骨关节炎的研究进展[J]. 河北中医,2017,39(12):1908-1912. doi: 10.3969/j.issn.1002-2619.2017.12.034 [4] 张立,董介轩,殷志荣,等. 针刀松解对坐骨神经痛大鼠中枢腓呔类物质影响研究[J]. 遵义医学院学报,2019,42(4):393-398. doi: 10.3969/j.issn.1000-2715.2019.04.008 [5] 郭长青,曹榕娟,孙红梅,等. 针刀松解法对第3腰椎横突综合征大鼠下丘脑前阿黑皮素mRNA、前脑啡肽原mRNA表达的影响[J]. 针刺研究,2010,35(5):354-358. [6] Duan H,Zhang Y,Zhang X M,et al. Antinociceptive roles of galanin receptor 1 in nucleus accumbens of rats in a model of neuropathic pain[J]. J Neurosci Res,2015,93(10):1542-1551. doi: 10.1002/jnr.23611 [7] Zhang Y,Gao Y,Li C Y,et al. Galanin plays a role in antinociception via binding to galanin receptors in the nucleus accumbens of rats with neuropathic pain[J]. Neurosci Lett,2019,706(7):93-98. [8] Hadley J A,Nenert R,Kraguljac N V,et al. Ventral tegmental area/midbrain functional connectivity and response to antipsychotic medication in schizophrenia[J]. Neuropsychopharmacol,2014,39(4):1020-1030. doi: 10.1038/npp.2013.305 [9] Cooper S,Robison A J,Mazei-Robison M S. Reward circuitry in addiction[J]. Neurotherapeutics,2017,14(3):687-697. doi: 10.1007/s13311-017-0525-z [10] Salgado S and Kaplitt M G. The nucleus accumbens:A comprehensive review[J]. Stereotact Funct Neurosurg,2015,93(2):75-93. doi: 10.1159/000368279 [11] Watanabe M,Sugiura Y,Sugiyama E,et al. Extracellular N-acetylaspartylglutamate released in the nucleus accumbens modulates the pain sensation:Analysis using a microdialysis/mass spectrometry integrated system[J]. Mol Pain,2018,14(6). [12] Bennett G J and Xie Y K. A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man[J]. Pain,1988,33(1):87-107. doi: 10.1016/0304-3959(88)90209-6 [13] 靳勇,蔡少忍. 小针刀疗法治疗腰椎间盘突出症的临床观察[J]. 中医临床研究,2019,11(1):29-30. doi: 10.3969/j.issn.1674-7860.2019.01.011 [14] 杨艺,谭龙旺,张勇. 硬膜外类固醇注射与小针刀治疗腰椎间盘突出症的疗效比较[J]. 江西中医药,2019,50(1):34-36. [15] 叶肖琳,黄雪莲,叶新苗. 小针刀对腰椎间盘突出症治疗效果的Meta分析[J]. 中华中医药杂志,2016,31(7):2784-2788. [16] 黄丙勇. 小针刀配合中药治疗神经根型颈椎病的临床观察[J]. 中国中医药现代远程教育,2018,16(22):138-140. doi: 10.3969/j.issn.1672-2779.2018.22.060 [17] 蔡润,肖洪波,罗金发,等. 探究小针刀治疗颈肩肌筋膜疼痛综合征的临床价值[J]. 解放军预防医学杂志,2019,37(6):75-76. [18] 谢敏娇,冯淑兰. 小针刀松解术治疗枕神经痛的临床研究[J]. 针灸临床杂志,2019,35(2):36-39. doi: 10.3969/j.issn.1005-0779.2019.02.012 [19] 张建军,丁宇,杨改平. 针刀治疗带状疱疹后神经痛疗效观察与分析[J]. 中国疼痛医学杂志,2017,23(5):389-391. doi: 10.3969/j.issn.1006-9852.2017.05.015 [20] 胡波,郭长青,孙红梅,等. 针刀疗法对腰三横突征模型大鼠局部组织IL-6、IL-10、TNF-α、TGF-β的影响[J]. 中华中医药杂志,2008,23(8):699-702. [21] Xu S L,Li J,Zhang J J,et al. Antinociceptive effects of galanin in the nucleus accumbens of rats[J]. Neurosci Lett,2012,520(1):43-46. doi: 10.1016/j.neulet.2012.05.027 [22] Yang Y,Zhang Y,Li X H,et al. Involvements of galanin and its receptors in antinociception in nucleus accumbens of rats with inflammatory pain[J]. Neurosci Res,2015,97(8):20-25. [23] Dong Y,Li C Y,Zhang X M,et al. The activation of galanin receptor 2 plays an antinociceptive effect in nucleus accumbens of rats with neuropathic pain[J]. J Physio Sci,2021,71(1):6. doi: 10.1186/s12576-021-00790-5 -
下载:
点击查看大图
图(4)
计量
- 文章访问数: 2581
- HTML全文浏览量: 1903
- PDF下载量: 18
- 被引次数: 0
