miRNA-145负向调控子宫内膜异位症中OCT4的表达

伍路; 赵庆华; 杨绍艳; 侯友芳; 张素仙

doi:10.12259/j.issn.2095-610X.S20211032

miRNA-145负向调控子宫内膜异位症中OCT4的表达

doi: 10.12259/j.issn.2095-610X.S20211032

昆明医科大学第二附属医院妇科，云南昆明　650101

基金项目: 云南省教育厅科学研究基金资助项目（2020J0183）；云南省科学技术厅-昆明医科大学联合专项基金资助项目（202001AY070001-227）；昆明医科大学第二附属医院院内基金资助项目（2018yk017）

详细信息

作者简介:
伍路（1988～），女，云南开远人，医学硕士，住院医师，主要从事妇科肿瘤及普通妇科工作

通讯作者:
张素仙，E-mail：zsx0902@126.com

中图分类号: R711.71
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出版历程
- 收稿日期: 2021-08-13
- 网络出版日期: 2021-10-29
- 刊出日期: 2021-10-30

MiRNA-145 Negatively Regulates OCT4 Expression in Endometriosis

Second Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650101，China

摘要

摘要: 目的探讨miRNA-145、OCT4在子宫内膜异位症（EMs）中的表达水平及临床意义。方法收集2018年8月至2020年8月昆明医科大学第二附属医院妇科收治的EMs患者30例（实验组）,不孕症（输卵管因素）患者30例（对照组），实验组术中取异位子宫内膜组织，对照组术中取在位子宫内膜组织，QPCR检测异位内膜组织、在位内膜组织中miRNA-145、OCT4的表达，Western印迹法检测组织中OCT4蛋白的表达。结果 EMs患者子宫内膜异位组织中较非EMs患者在位内膜组织中miRNA-145表达增高（P < 0.01），而OCT4表达下调（P < 0.01），2者呈现负相关关系。结论 miRNA-145有望成为子宫内膜异位症诊断的潜在标志物。miRNA-145与OCT4在子宫内膜异位症发生、发展中具有一定的作用，这一发现为EMs的发病机制及治疗提供了可能的研究方向及潜在靶点。
- 子宫内膜异位症 /
- miRNA-145 /
- OCT4 /
- 在位子宫内膜 /
- 异位子宫内膜
Abstract: Objective To study the expression and clinical significance of miRNA-145 and OCT4 in endometriosis. Methods From August 2018 to August 2020, 30 EMs patients （experimental group） and 30 patients with infertility caused by fallopian tube factors （control group） were collected. Ectopic endometrial tissues were collected during operation in experimental group and eutopic endometrial tissues in control group. The expressions of miRNA-145 and OCT4 in ectopic and eutopic endometrial tissues were detected by QPCR, and the expression of OCT4 protein in tissues was detected by Western blot. Results Compared with non-EMs patients, the expression of miRNA-145 in endometriotic tissues increased （P < 0.01）, while the expression of OCT4 decreased （P < 0.01）, showing a negative correlation between them. Conclusion miRNA-145 is expected to be a potential marker for diagnosis of endometriosis. miRNA-145 and OCT4 play a certain role in the occurrence and development of endometriosis, and this finding provides a possible research direction and potential target for the pathogenesis and treatment of EMS.
- Endometriosis /
- miRNA-145 /
- OCT4 /
- Eutopic endometrium /
- Ectopic endometrium

HTML全文

图 1 western blotting检测EU和EC中OCT4的表达水平

^***P < 0.001。

Figure 1. OCT4 expression in EC and EU tissues of patients with EMs was investigated using western blotting

下载: 全尺寸图片幻灯片

图 2 EMs患者异位内膜组织中miR-145与OCT4相关关系

Figure 2. Correlation between miR-145 and OCT4 in EMs patients

下载: 全尺寸图片幻灯片

表 1 QPCR扩增引物序列表

Table 1. Nucleotide sequences of the primers used for RT-PCR

引物名称	引物序列
miR-145-5p forward	5′ -GTC CACCGCAAATGCTTCTA-3′
miR-145-5P reverse	5′ -CCATCAGTCCCGTCTTGAAAC-3′
OCT4 forward	5′ -GTCCACCGC AAATGCTTCTA-3′
OCT4 reverse	5′ -CCATCAGTCCCGTCT TGAAAC-3
GAPDH forward	5′ -TTCCAGGAGCGAGATCCCT-3′
GAPDH reverse	5′ -CACCCATGACGAACATGGG-3′ ;
U6 forward	5′ -CTCGCTTCGGCAGCACAT-3′
U6 reverse	5′ -AACGCTTCACGAATTTGCGT-3′ .

下载: 导出CSV

表 2 2组患者中miR-145表达水平（$\bar x \pm s $）

Table 2. Expression level of miR-145 in two groups of patients （$\bar x \pm s $）

组别	n	miR-145	t	P
EC组	30	1.64 ± 0.74	3.459	0.001
EU组	30	1.00 ± 0.52	3.459	0.001

下载: 导出CSV

表 3 2组患者中OCT4表达水平（$\bar x \pm s $）

Table 3. Expression level of OCT4 in two groups of patients （$\bar x \pm s $）

组别	n	OCT4	t	P
EC组	30	0.68 ± 0.44	2.710	0.009
EU组	30	1.01 ± 0.52	2.710	0.009

下载: 导出CSV

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