七氟烷抑制宣威肺癌XWLC-05细胞生物学行为

全宇航; 王忠慧; 李珊珊; 刘光顺

doi:10.12259/j.issn.2095-610X.S20211202

七氟烷抑制宣威肺癌XWLC-05细胞生物学行为

doi: 10.12259/j.issn.2095-610X.S20211202

昆明医科大学第三附属医院/云南省肿瘤医院麻醉科，云南昆明　650118

基金项目: 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目（2018FE001-070；2019FE001-248）

详细信息

作者简介:
全宇航（1983～），男，河北唐山人，麻醉学硕士，主治医师，主要从事麻醉药物与肿瘤的临床及科研工作

通讯作者:
王忠慧，E-mail：skywz911@sina.com

中图分类号: R734.2;R730.2
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出版历程
- 收稿日期: 2021-10-05
- 网络出版日期: 2021-11-16
- 刊出日期: 2021-12-15

Sevoflurane Inhibits Xuanwei Lung Cancer Cell Line XWLC-05

Dept. of Anesthesiology ，The 3rd Affiliated Hospital of Kunming Medical University/Cancer Hospital of Yunnan Province，Kunming Yunnan 650118，China

摘要

摘要: 目的了解七氟烷对宣威肺癌细胞XWLC-05增殖、迁移、侵袭及恶性行为的影响，并进一步探讨其潜在机制。方法七氟烷对XWLC-05细胞进行处理，体外实验检测XWLC-05细胞增殖、凋亡、迁移和侵袭变化；体内实验检测XWLC-05细胞致瘤性，蛋白印迹法分析增殖关键分子PI3K/AKT、细胞侵袭蛋白MMP-2/MMP-9的表达。结果对照组的克隆形成数（287.5±23.1）个，高于实验组（174.1±16.3）个，（P < 0.05）；对照组的凋亡率（1.60±0.38）%，低于实验组（31.48±5.81）%，（P < 0.01）；对照组迁移细胞数（87.8±10.2）%，高于实验组（35.2±4.4）%，（P < 0.05）；对照组侵袭细胞数（93.6±7.3）个，高于实验组（30.7±3.2）个，（P < 0.01）。七氟烷处理下调增殖蛋白分子PI3K、p-AKT和侵袭蛋白分子MMP-2、MMP-9的表达水平（P < 0.05）。结论七氟烷通过抑制PI3K/AKT信号通路降低宣威肺癌细胞XWLC-05的增殖能力，诱导细胞凋亡，减弱XWLC-05细胞的迁移、侵袭和成瘤能力。七氟烷可能成为宣威肺癌治疗的一种新方法。
- 七氟烷 /
- 宣威肺癌 /
- 细胞凋亡 /
- 细胞增殖
Abstract: Objective To investigate the effects of sevoflurane on proliferation, migration, invasion and malignant behavior of Xuanwei lung cancer cells XWLC-05, and to further explore its potential mechanism. Method XWLC-05 cells were treated with sevoflurane, and the changes of proliferation, apoptosis, migration and invasion of XWLC-05 cells were detected in vitro; the tumorigenicity of XWLC-05 cells was detected in vivo, and the expressions of PI3K/AKT and MMP-2 / MMP-9 were analyzed by Western blot. Result The number of clones in control group （287.5±23.1） was higher than that in experimental group （174.1±16.3）（P < 0.05）; the apoptosis rate in the control group （1.60±0.38） was significantly lower than that in the experimental group （31.48±5.81）（P < 0.01）, and the number of migrating cells in the control group （87.8±10.2） was significantly higher than that in the experimental group （35.2±4.4）（P < 0.05）. The number of invasive cells in the control group（93.6±7.3） was significantly higher than that in the experimental group （30.7±3.2）（P < 0.01）. Sevoflurane treatment significantly decreased the expression levels of PI3K, p-Akt and MMP-2, MMP-9 （P < 0.05）. Conclusion By inhibiting PI3K/AKT signaling pathway, sevoflurane decreased the proliferation ability of XWLC-05, induced apoptosis, and weakened the migration, invasion and tumorigenesis ability of XWLC-05 cells. Sevoflurane may be a new treatment option for Xuanwei lung cancer.
- Sevoflurane /
- Xuanwei lung cancer /
- Apoptosis /
- Cell proliferation

HTML全文

图 1 XWLC-05细胞克隆形成结果

与对照组相比，^**P < 0.01 。

Figure 1. XWLC-05 cell clone in two groups

下载: 全尺寸图片幻灯片

图 2 七氟烷处理后XWLC-05细胞凋亡结果

与对照组相比，^**P < 0.01。

Figure 2. Apoptosis of XWLC-05 cells after sevoflurane treatment

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图 3 XWLC-05细胞迁移和侵袭检测（×100）

Figure 3. XWLC-05 cell migration and invasion （×100）

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图 4 XWLC-05细胞迁移和侵袭检测统计

与对照组相比，^*P < 0.05，^**P < 0.01。

Figure 4. XWLC-05 cell migration and invasion detection statistics

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图 5 XWLC-05细胞成瘤后瘤体生长曲线

与对照组相比，^**P < 0.01。

Figure 5. Growth curve of XWLC-05 cells after tumorigenesis

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图 6 关键分子蛋白表达检测

Figure 6. Expression of key molecular proteins

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图 7 瘤体组织中关键分子蛋白表达检测统计

与对照组相比，^*P < 0.05，^**P < 0.01。

Figure 7. Detection and statistics of key molecular protein expression in tumor tissue

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参考文献(25)

[1]	Xiao Y,Shao Y,Yu X,et al. The epidemic status and risk factors of lung cancer in Xuanwei City,Yunnan Province,China[J]. Frontiers of Medicine,2012,6(4):388. doi: 10.1007/s11684-012-0233-3
[2]	吕任齐,赵体德. 云南省9县市肺癌死亡率趋势分析[J]. 中国肿瘤,2002,11(12):700-701. doi: 10.3969/j.issn.1004-0242.2002.12.014
[3]	鲁菱娇. 云南宣威肺癌circRNAs标志物的初步筛选及表达验证[D]. 昆明: 昆明医科大学硕士学位论文, 2018.
[4]	Li J,He J,Tang R,et al. Molecular epidemiology study in Xuanwei:The relationship among coal type,genotype and lung cancer risk[J]. Zhong Guo Fei Ai Za Zhi,2015,18(1):16-22.
[5]	Cui J,Zhang F,Wang Y,et al. Macrophage migration inhibitory factor promotes cardiac stem cell proliferation and endothelial differentiation through the activation of the PI3K/Akt/mTOR and AMPK pathways[J]. Int J Mol Med,2016,37(5):1299-1309. doi: 10.3892/ijmm.2016.2542
[6]	Ma H,Su R,Feng H,et al. Long noncoding RNA UCA1 promotes osteosarcoma metastasis through CREB1-mediated epithelial-mesenchymal transition and activating PI3K/AKT/mTOR pathway[J]. J Bone Oncol,2019,16(6):100228.
[7]	Qin Y,Ni J,Kang L,et al. Sevoflurane effect on cognitive function and the expression of oxidative stress response proteins in elderly patients undergoing radical surgery for lung cancer[J]. J Coll Physicians Surg Pak,2019,29(1):12-15. doi: 10.29271/jcpsp.2019.01.12
[8]	Hamshaw I,Ajdarirad M,Mueller A. The role of PKC and PKD in CXCL12 directed prostate cancer migration[J]. Biochem Biophys Res Commun,2019,519(1):86-92. doi: 10.1016/j.bbrc.2019.08.134
[9]	Gupta R,Santha N,Upadya M,et al. Effect of different dosages of intravenous midazolam premedication on patients undergoing headand neck surgeries- A double blinded randomized controlled study[J]. J Clin Diagn Res,2017,11(8):1-04.
[10]	姚毕,张伟强,张波太,等. 不同剂量咪达唑仑对肺癌手术患者的麻醉效果研究[J]. 中外医学研究,2017,15(33):57-58.
[11]	Jiang K,Li J,Zhang J,et al. SDF-1/CXCR4 axis facilitates myeloid-derived suppressor cells accumulation in osteosarcoma microenvironment and blunts the response to anti-PD-1 therapy[J]. Int Immunopharmacol,2019,75(2):e105818.
[12]	程慧慧,张文文,朱雪琼. 七氟烷影响恶性肿瘤生物学行为的研究进展[J]. 浙江医学,2017,39(20):1813-1816,1818. doi: 10.12056/j.issn.1006-2785.2017.39.20.2016-2050
[13]	Bray F,Ferlay J,Soeijomataram I,et al. Global cancer statistics 2018:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA:A Cancer Journal for Clinicians,2018,68(6):394-424. doi: 10.3322/caac.21492
[14]	Sung H,Ferlay J,Siegel R L,et al. Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin,2021,71(3):209-249. doi: 10.3322/caac.21660
[15]	Zhang X,Liu S,Liu Y,et al. Economic burden for lung cancer survivors in urban China[J]. Int J Environ Res Public Health,2017,14(3):308. doi: 10.3390/ijerph14030308
[16]	He W,Yang T,Gong X H,et al. Targeting CXC motif chemokine receptor 4 inhibits the proliferation,migration and angiogenesis of lung cancer cells[J]. Oncol Lett,2018,16(3):3976-3982.
[17]	Huang W,Zhao S,Xu W,et al. Presence of intra-tumoral CD61+ megakaryocytes predicts poor prognosis in non-small cell lung cancer[J]. Transl Lung Cancer Res,2019,8(4):323-331. doi: 10.21037/tlcr.2019.08.23
[18]	Ierano C,D'alterio C,Giarra S,et al. CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells[J]. Cell Death Dis,2019,10(8):562. doi: 10.1038/s41419-019-1796-6
[19]	Li B,Wang Z,Wu H,et al. Epigenetic regulation of CXCL12 Plays a critical role in mediating tumor progression and the immune response in osteosarcoma[J]. Cancer Res,2018,78(14):3938-3953. doi: 10.1158/0008-5472.CAN-17-3801
[20]	Marques C S,Santos A R,Gameiro A,et al. CXCR4 and its ligand CXCL12 display opposite expression profiles in feline mammary metastatic disease,with the exception of HER2-over expressing tumors[J]. BMC Cancer,2018,18(1):741. doi: 10.1186/s12885-018-4650-9
[21]	Schiano C,Soricelli A,De Nigris F,et al. New challenges in integrated diagnosis by imaging and osteoimmunology in bone lesions[J]. Expert Rev Clin Immunol,2019,15(3):289-301. doi: 10.1080/1744666X.2019.1561283
[22]	Sherman H,Gitschier H J,Rossi A E. A novel three-dimensional immune oncology model for high-throughput testing of tumoricidal activity[J]. Front Immunol,2018,9(11):857-868.
[23]	Zhang J,Chen J,Wo D,et al. LRP6 ectodomain prevents SDF-1/CXCR4-induced breast cancer metastasis to lung[J]. Clin Cancer Res,2019,25(15):4832-4845. doi: 10.1158/1078-0432.CCR-18-3557
[24]	Hsu M J,Peng S F,Chueh F S,et al. Lupeol suppresses migration and invasion via p38/MAPK and PI3K/Akt signaling pathways in human osteosarcoma U2OS cells[J]. Biosci Biotechnol Biochem,2019,22(3):1-11.
[25]	Hung C Y,Lee C H,Chiou H L,et al. Praeruptorin-B inhibits 12-O-tetradecanoylphor-bol-13- acetate-induced cell invasion by targeting AKT/NF-κB via matrix metalloproteinase-2/-9 expression in human cervical cancer cells[J]. Cell Physiol Biochem,2019,52(6):1255-1266.

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