碘-125粒子调控微小RNA-193b-5p抑制胃癌的增殖和侵袭

马振桓; 李震; 周香林; 李国剑; 杨国凯; 万嘉; 杜玲娟; 杨镛

doi:10.12259/j.issn.2095-610X.S20220120

碘-125粒子调控微小RNA-193b-5p抑制胃癌的增殖和侵袭

doi: 10.12259/j.issn.2095-610X.S20220120

马振桓^{1, 2, 3},
李震^{1, 2},
周香林³,
李国剑^{1, 2},
杨国凯^{1, 2},
万嘉^{1, 2},
杜玲娟^{1, 2},
杨镛^{1, 2, ,}

1.
云南大学附属医院普外三科，云南昆明　650021
2.
昆明医科大学第四附属医院普外三科，云南昆明　650500
3.
大理大学临床医学院，云南大理　671003

基金项目: 云南省卫生科技基金资助项目（2017NS137，2018NS0008，2018NS0009）；云南省高层次卫生健康技术人才培养专项基金资助项目（H-2017017）；云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目（2018FE001（-076））

详细信息

作者简介:
马振桓（1969～），男，云南昆明人，医学博士，副主任医师，主要从事肿瘤外科、血管外科临床工作

李震与马振桓对本文有同等贡献

通讯作者:
杨镛，E-mail：yncvs126@126.com

中图分类号: R735.2
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- 被引次数: 0
出版历程
- 收稿日期: 2021-12-27
- 网络出版日期: 2021-12-20
- 刊出日期: 2022-01-15

Iodine-125 Seed Inhibits the Proliferation and Invasion of Gastric Cancer Cells by Regulating MicroRNA-193b-5p Expression

Zhenhuan MA^{1, 2, 3},
Zhen LI^{1, 2},
Xianglin ZHOU³,
Guojian LI^{1, 2},
Guokai YANG^{1, 2},
Jia WAN^{1, 2},
Lingjuan DU^{1, 2},
Yong YANG^{1, 2
, ,}

1.
The 3rd Department. of General Surgery，The Affiliated Hospital of Yunnan University，Kunming Yunnan 650021
2.
The 3rd Department of General Surgery，The 4th Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650021
3.
Clinical Medical College，Dali University，Dali Yunnan 671003，China

摘要

摘要: 目的探究碘-125粒子放疗胃癌过程中微小RNA（miR）-193b-5p的作用。方法以胃癌细胞系BGC-823作为对象，建立碘-125粒子体外照射模型，采用小分子RNA测序技术检测miRNA的表达谱。通过实时定量PCR检测碘-125辐照组和非辐照组细胞中miR-193b-5p的表达水平，使用hsa-miR-193b-5p抑制物和相应阴性抑制物转染胃癌细胞系BGC-823建立miR-193b-5p敲减细胞和对照细胞（miR-193b-5p敲减组和对照组），采用噻唑蓝（MTT）检测2组细胞增殖情况，采用Transwell分析2组细胞的侵袭情况。结果碘-125辐照组细胞miR-193b-5p表达水平（0.853±0.180）显著高于非辐照组（0.173±0.045），差异有统计学意义（t = 6.371，P < 0.05）；敲减组细胞miR-193b-5p表达水平（1.264±0.311）明显低于对照组（12.219±2.464），差异有统计学意义（t = -7.640，P < 0.05）；miR-193b-5p敲减组细胞的增殖能力（0.574±0.382）明显高于对照组细胞（0.424±0.246），差异有统计学意义（t=3.927，P < 0.05）；miR-193b-5p敲减组细胞侵袭数量（222±23）明显高于对照组细胞侵袭数（40±7），差异有统计学意义（t = -13.293，P < 0.05）。结论碘-125放疗胃癌过程中，能通过调控miR-193b-5p的表达抑制胃癌细胞的增殖和侵袭。
- 碘-125粒子 /
- 胃癌 /
- 微小RNA-193b-5p /
- 细胞增殖 /
- 细胞侵袭
Abstract: Objective To investigate the role of microRNA （miRNA, miR）-193b-5p in the process of iodine-125 particle radiotherapy for gastric cancer. Methods Gastric cancer cell line BGC-823 was used as the research object and iodine-125 seed in vitro irradiation model was established, and the expression profile of miRNA was detected by small molecule RNA sequencing. The expression of miR-193b-5p was analyzed by quantitative real time polymerase chain reaction （RT-qPCR）. MiR-193b-5p knockdown cell and control cell lines （knockdown group and control group） were established in gastric cancer cell line BGC-823 by hsa-miR-193b-5p inhibitor and NC inhibitor. Cell proliferation was analyzed by methyl thiazolyl tetrazolium （MTT） assay in the two groups. Cell invasion was analyzed by Transwell. Result Compared with the expression level of miR-193b-5p in the non-irradiated group （0.173±0.045）, the expression of miR-193b-5p in the iodine-125 irradiated group （0.853±0.180） was significantly increased （t = 6.371, P < 0.05）. Compared with the expression of miR-193b-5p in the control group （12.219±2.464）, the expression of miR-193b-5p in the knockdown group （1.264±0.311） was significantly decreased （t = -7.640, P < 0.05）. Compared with the control group （0.424±0.246）, the proliferation of knockdown group （0.574±0.382）was significantly increased （t = 3.927, P < 0.05）. Compared with the control group （40±7）, the number of cell invasion in the knockdown group （222+23） was significantly increased （t = -13.293, P < 0.05）. Conclusion Iodine-125 seed suppresses the proliferation and invasion of gastric cancer cells by regulating the expression of miR-193b-5p in the process of radiotherapy for gastric cancer.
- Iodine-125 seed /
- Gastric cancer /
- microRNA-193b-5p /
- Cell proliferation /
- Cell invasion

HTML全文

图 1 MTT检测敲低miR-193b-5p对BGC-823细胞增殖的影响

Con：阴性对照组；KD：miR-193b-5p敲减组。

Figure 1. The effect of knocking down miR-193b-5p on the proliferation of BGC-823 cells by MTT method

下载: 全尺寸图片幻灯片

图 2 Transwell实验检测敲低miR-193b-5p对BGC-823细胞侵袭的影响

A：对照组与miR-193b-5p敲减组的细胞侵袭情况（×400）；B：2组侵袭细胞数的t检验。Con：阴性对照组；KD：miR-193b-5p敲减组。与阴性对照组比较，^***P < 0.001。

Figure 2. The effect of knocking down miR-193b-5p on the invasion of BGC-823 cells by transwell experiment

下载: 全尺寸图片幻灯片

表 1 小分子RNA测序差异表达microRNAs结果

Table 1. Differentially expressed microRNAs identified by next generation sequencing

成熟的miRNA	miRNA前体	成熟的RNA序列	差异倍数	P	FDR	调节
hsa-miR-17-5p	hsa-mir-17	CAAAGUGCUUACAGUGCAGGUAG	2.074	0.029	0.630	up
hsa-miR-193b-5p	hsa-mir-193b	CGGGGUUUUGAGGGCGAGAUGA	7.000	0.049	0.630	up
hsa-miR-23b-5p	hsa-mir-23b	UGGGUUCCUGGCAUGCUGAUUU	1.750	0.000	0.000	up
hsa-miR-483-5p	hsa-mir-483	AAGACGGGAGGAAAGAAGGGAG	3.569	0.033	0.630	up
hsa-miR-92a-1-5p	hsa-mir-92a-1	AGGUUGGGAUCGGUUGCAAUGCU	5.600	0.007	0.630	up
hsa-miR-127-3p	hsa-mir-127	UCGGAUCCGUCUGAGCUUGGCU	−2.033	0.036	0.630	down
hsa-miR-1285-3p	hsa-mir-1285-1	UCUGGGCAACAAAGUGAGACCU	−2.000	0.000	0.000	down
hsa-miR-1285-3p	hsa-mir-1285-2	UCUGGGCAACAAAGUGAGACCU	−2.000	0.000	0.000	down
hsa-miR-296-5p	hsa-mir-296	AGGGCCCCCCCUCAAUCCUGU	−2.667	0.038	0.630	down
hsa-miR-421	hsa-mir-421	AUCAACAGACAUUAAUUGGGCGC	−1.750	0.000	0.000	down
hsa-miR-495-3p	hsa-mir-495	AAACAAACAUGGUGCACUUCUU	−7.714	0.034	0.630	down
hsa-miR-548am-3p	hsa-mir-548am	CAAAAACUGCAGUUACUUUUGU	−3.333	0.020	0.630	down

下载: 导出CSV

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