GDM孕妇网膜脂肪组织中Chemerin的表达与IRS-1及其酪氨酸磷酸化分析

李晓红; 赵琳; 张馨文; 陈卓; 马润玫

doi:10.12259/j.issn.2095-610X.S20220140

GDM孕妇网膜脂肪组织中Chemerin的表达与IRS-1及其酪氨酸磷酸化分析

doi: 10.12259/j.issn.2095-610X.S20220140

昆明医科大学第一附属医院产科，云南昆明　650032

基金项目: 国家自然科学基金项目资助项目（81160082）；云南省科技厅-昆明医科大学应用基础联合专项基金资助项目（202001AC070082）；博士科研启动基金资助项目（2016BS004）

详细信息

作者简介:
李晓红（1976～），女，山西永济人，医学博士，副主任医师，主要从事产科临床及科研工作

通讯作者:
马润玫，E-mail：marunmeikmmc@163.com

中图分类号: R714.2
计量
- 文章访问数: 3873
- HTML全文浏览量: 1940
- PDF下载量: 102
- 被引次数: 0
出版历程
- 收稿日期: 2021-12-06
- 网络出版日期: 2022-01-15
- 刊出日期: 2022-01-15

Analysis of Chemerin Expression and IRS-1 and Tyrosine Phosphorylation in Omental Adipose Tissue of GDM Pregnant Women

Dept. of Obstetrics and Gynecology，The 1st Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

摘要

摘要: 目的通过检测网膜脂肪组织中Chemerin的表达与胰岛素受体底物-1表达及酪氨酸磷酸化水平，探讨Chemerin与GDM胰岛素抵抗的关系。方法将研究对象分为GDM组及正常对照组NGT各22名，剖宫产术中收集网膜脂肪组织2g，Western blot及qPCR方法检测胎盘组织中Chemerin、IRS-1的表达及免疫沉淀法检测IRS-1的酪氨酸磷酸化水平。结果 GDM组网膜脂肪组织中Chemerin蛋白质表达显著增加[2.99 （0.04，7.90） vs 2.21（0.02，13.30），P = 0.010]；GDM组IRS-1[3.52（2.51，4.49）vs 5.47（4.04，8.98），P = 0.024]蛋白表达明显降低；GDM组IRS-1酪氨酸磷酸化[0.60（0.01，1.74） vs 4.06（0.06，54.41），P = 0.012]显著降低；相关性分析发现Chemerin 蛋白表达与OGTT2h血糖密切相关；Chemerin mRNA与产前BMI密切相关；Chemerin蛋白表达与IRS-1蛋白正相关；IRS-1酪氨酸磷酸化水平与OGTT 0 h PG、OGTT 2 h PG密切负相关。结论网膜脂肪组织中Chemerin表达与IRS-1信号异常密切相关是其引起GDM胰岛素抵抗因素之一。
- GDM /
- Chemerin /
- IRS-1 /
- 酪氨酸磷酸化 /
- 胰岛素抵抗
Abstract: Objective To investigate the relationship between chemerin and insulin resistance in GDM by detecting the expression of insulin receptor substrate-1 and tyrosine phosphorylation in omental adipose tissue. Methods Subjects were divided into the GDM group 24 and the normal control group with 22 NGT. Two grams of omental adipocytes were collected during the cesarean section. The expression of chemerin and IRS-1 in placental tissues was detected with Western blot and qPCR, and the tyrosine phosphorylation of IRS-1 was detected with the immunoprecipitation. Results The expression of chemerin protein in retinal adipocytes in GDM group was significantly increased （2.99 （0.04, 7.90） vs 2.21 （0.02, 13.30）, P = 0.010）. The protein expression of IRS-1 （3.52（2.51, 4.49） vs 5.47 （4.04, 8.98）, P = 0.024） was significantly decreased in GDM group. The phosphorylation of IRS-1 tyrosine was significantly decreased in GDM group （0.60（0.01, 1.74） vs 4.06 （0.06, 54.41）, P = 0.012）. Correlation analysis showed that chemerin protein expression was closely related to OGTT2h glucose. Chemerin mRNA was closely correlated with prenatal BMI. Chemerin protein expression was positively correlated with IRS-1 protein. IRS-1 tyrosine phosphorylation was negatively correlated with OGTT 0 h PG and OGTT 2 h PG. Conclusion The expression of chemerin in omental adipose tissue is closely related to IRS-1 signaling, which is one of the factors causing insulin resistance in GDM.
- GDM /
- Chemerin /
- IRS-1 /
- Tyrosine phosphorylation /
- Insulin resistance

HTML全文

图 1 网膜脂肪组织中Chemerin蛋白的相对表达

Figure 1. The relative expression of Chemerin in omental adipose tissue

下载: 全尺寸图片幻灯片

图 2 网膜脂肪组织中IRS-1mRNA及蛋白的表达

Figure 2. The relative expression of IRS-1 mRNA and protein in omental adipose tissue

下载: 全尺寸图片幻灯片

图 3 网膜脂肪组织中IRS-1酪氨酸磷酸化表达

Figure 3. The tyrosine phosphorylation of IRS-1 in omental adipose tissue

下载: 全尺寸图片幻灯片

图 4 网膜脂肪组织中Chemerin、IRS-1及酪氨酸磷酸化表达

1，2列是 GDM；3，4 列 NGT。

Figure 4. The relative expression of Chemerin，IRS-1 and tyrosine phosphorylation in omental adipose tissue

下载: 全尺寸图片幻灯片

表 1 GDM组孕妇与对照组一般临床资料比较

Table 1. Comparison of general clinical data between GDM group and control group

一般项目	GDM组（n = 30）	NGT组（n = 24）	t	P
年龄（岁）	30.23 ± 4.40	28.77 ± 4.76	1.142	0.259
孕周（d）	266 ± 3.42	266 ± 5.31	−0.125	0.872
孕前BMI（kg/m²）	22.03 ± 4.24	20.64 ± 2.24^*	−2.068	0.042
产前BMI（kg/m²）	27.62 ± 3.37	26.93 ± 2.76	−1.165	0.253
OGTT0h（mmol/L）	4.90 ± 0.84	4.29 ± 0.50^*	−4.430	0.000
OGTT2h（mmol/L）	8.37 ± 1.69	6.06 ± 1.05^*	−8.367	0.000
TC（mmol/L）	5.98 ± 0.93	5.69 ± 0.70		0.588
TG（mmol/L）	3.33（1.78，5.97）	3.70（1.71，11.89）		0.989
HDL（mmol/L）	1.57 ± 0.24	1.70 ± 0.32		0.283
LDL（mmol/L）	3.44 ± 0.82	3.01 ± 1.17		0.428
与GDM组比较，^P* < 0.05

下载: 导出CSV

参考文献(17)

[1]	程飞,郭晓蕙,杨慧霞,等. 妊娠糖尿病患者血清脂联素水平及其临床意义[J]. 北京医学,2009,6(31):337-339.
[2]	李晓红,马润玫,陈卓. 肥胖、胰岛素抵抗对妊娠期糖尿病患者血清网膜素-1水平的影响[J]. 昆明医科大学学报,2017,38(5):64-67. doi: 10.3969/j.issn.1003-4706.2017.05.014
[3]	A A Roman,S D Parlee,C J Sinal,et al. Chemerin:A potential endocrine link between obesity and type 2 diabetes[J]. Endocrine,2012,2(42):243-251.
[4]	S S Fatima,R Rehman,M Baig,et al. New roles of the multidimensional adipokine:Chemerin[J]. Peptides,2014,12(62):15-20.
[5]	Ferland D J,Mullick A E,Watts S W. Chemerin as a driver of hypertension:A consideration[J]. American Journal of Hypertension,2020,33(11):975-986. doi: 10.1093/ajh/hpaa084
[6]	Wang D,Wang H,Li M,et al. ,Chemerin levels and its genetic variants are associated with gestational diabetes mellitus:A hospital-based study in a Chinese cohort[J]. Gene,2021,10(8):145888E.
[7]	李晓红,陈卓,马润玫. 血清趋化素与妊娠期肥胖及胰岛素抵抗的关系[J]. 妇女保健,2013,28(36):5941-5944.
[8]	Michiko Takahashi,Yutaka Takahashi,Kenichi Takahashi,et al. Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes[J]. FEBS Letters,2008,582(5):573-578. doi: 10.1016/j.febslet.2008.01.023
[9]	Yashdeep G,Bharti K,Manash PB,et al. Updated guidelines on screening for gestational diabetes[J]. International Journal of Women’s Health,2015,19(7):539-550.
[10]	Jordan S D,Krüger M,Willmes D M,et al. Obesity-induced over-expression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism[J]. Nat Cell Biol,2011,13(4):434-446. doi: 10.1038/ncb2211
[11]	Hyun-Young Shin,Duk C Lee,Sang H Chu,et al. Chemerin levels are positively correlated with abdominal visceral fat accumulation[J]. Clinical Endocrinology,2012,77(1):47-50. doi: 10.1111/j.1365-2265.2011.04217.x
[12]	Anna Szpakowicz,Malgorzata Szpakowicz,Magda Lapinska,et al. Serum chemerin concentration is associated with proinflammatory status in chronic coronary syndrome[J]. Biomolecules,2021,11(8):1149. doi: 10.3390/biom11081149
[13]	Sabrina Bauer,Margarita Bala,Andrea Kopp,et al. Adipocyte chemerin release is induced by insulin without being translated to higher levels in vivo[J]. Eur J Clin Invest,2012,42(11):1213-1220. doi: 10.1111/j.1365-2362.2012.02713.x
[14]	Sabrina Bauer,Josef Wanninger,Sandra Schmidhofer,et al. Sterol regulatory element-binding protein 2 (SREBP2) activation after excess triglyceride storage induces chemerin in hypertrophic adipocytes[J]. Endocrinology,2011,152(1):26-35. doi: 10.1210/en.2010-1157
[15]	Kennedy A J,Davenport A P. Chemerin receptors CMKLR1 (Chemerin1) and GPR1 (Chemerin2) nomenclature,pharmacology,function[J]. Pharmacol,2018,70(1):174-196.
[16]	Koelman L,Reichmann R,Börnhorst C,et al. Determinants of elevated chemerin as a novel biomarker of immunometabolism:Data from a large population-based cohort[J]. Endocrine Connections,2021,20(9):1200-1211.
[17]	Ays, Bulut ,Gülçin Akca ,et al. The significance of blood and salivary oxidative stress markers and chemerin in gestational diabetes mellitus[J]. Taiwan residents Journal of Obstetrics & Gynecology,2021,4(60):695-699.

相关文章(20)

[1]	邓绍友, 赵玉兰, 王佩锦, 李蓉, 李进涛, 郑红. 恒古骨伤愈合剂联合广谱抗生素改善db/db小鼠胰岛素抵抗和肠道菌群, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230530
[2]	何银东, 张峻, 黄桦, 柳汝明, 王茜. 人类胎盘的葡萄糖转运机制及GDM对胎盘葡萄糖转运的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220903
[3]	范译丹, 陈玉, 饶春梅, 高雪娟, 张芳, 方山丹, 向润清, 范源, 吴洋. T2DM患者合并非酒精性脂肪肝与胰岛β细胞功能和胰岛素抵抗的关系, 昆明医科大学学报.
[4]	李晓红, 马润玫, 陈卓. 肥胖、胰岛素抵抗对妊娠期糖尿病患者血清网膜素-1水平的影响, 昆明医科大学学报.
[5]	蒋婷婷. 脂肪因子Vaspin、Apelin及瘦素与多囊卵巢综合征的相关性, 昆明医科大学学报.
[6]	桂琦. 血清中脂肪因子Apelin、Vaspin、瘦素与子宫内膜癌的相关性, 昆明医科大学学报.
[7]	王若天. 加速康复外科对结直肠癌患者术后胰岛素信号传导通路中TRB3表达, 昆明医科大学学报.
[8]	鲍娟. 帕金森病患者轻度认知损害与胰岛素抵抗的相关性, 昆明医科大学学报.
[9]	范敏娟. 瘦素、胰岛素抵抗与阻塞性睡眠呼吸暂停低通气综合征关系的研究, 昆明医科大学学报.
[10]	范敏娟. nCPAP治疗对OSAHS患者血清瘦素水平的影响, 昆明医科大学学报.
[11]	白永. 天麻素对胰岛素抵抗模型中CBS表达的影响, 昆明医科大学学报.
[12]	张媛. 2型糖尿病合并非酒精性脂肪肝病患者Chemerin与氧化应激变化及临床意义, 昆明医科大学学报.
[13]	蒋建文. 高血压颈动脉硬化患者血清Chemerin与MMPs及血管内皮功能关系, 昆明医科大学学报.
[14]	沈丽新. 非酒精脂肪肝病患者ApoB/ApoA1比值与胰岛素抵抗的相关性研究, 昆明医科大学学报.
[15]	李若楠. 三七总皂苷调控c-Jun氨基末端激酶改善大鼠肝组织胰岛素抵抗的作用, 昆明医科大学学报.
[16]	刘淑清. 2型糖尿病胰岛素抵抗与认知功能障碍关系研究, 昆明医科大学学报.
[17]	丘红梅. 代谢综合征患者胰岛素抵抗与血浆醛固酮相关性研究, 昆明医科大学学报.
[18]	. 正常妊娠、妊娠期糖尿病人的胰岛素抵抗状态及胰岛素分泌功能探讨, 昆明医科大学学报.
[19]	. 持续气道正压通气治疗对OSAHS患者胰岛素抵抗的影响, 昆明医科大学学报.
[20]	桂莉. 2型糖尿病大鼠骨骼肌氧化应激与胰岛素抵抗的关系, 昆明医科大学学报.