Study on Microbiome of Supragingival Plaque of College Students with Gingivitis
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摘要:
目的 采用高通量测序技术比较牙龈健康大学生和牙龈炎大学生龈上菌斑微生物差异。 方法 按照纳入、排除标准，在昆明医科大学招募18～25岁牙龈健康大学生30名、牙龈炎大学生33名，采用MiSeq系统对16S rDNA基因扩增子进行测序，比较2组受试者龈上菌斑的多样性、结构和组成。 结果 共检测到87，595个序列，其中包含14个门216个属。与牙龈健康组相比，牙龈炎组受试者的龈上菌斑中α多样性指标较高，差异具有统计学意义（P < 0.001）。相似性分析（Anosim）和主成分分析（PCA）显示牙龈健康组和牙龈炎组受试者的龈上菌斑微生物群落结构、组成存在差异，且差异具有统计学意义（P < 0.001）。2组受试者龈上菌斑群落的相对丰度存在差异，牙龈炎组中普氏菌属、卟啉单胞菌属、杆菌属的相对丰度较高，而牙龈健康组中放线菌属的相对丰度较高。 结论 牙龈炎组和牙龈健康组大学生受试者龈上菌斑的微生物群落组成和结构存在差异，牙龈炎组龈上菌斑的多样性较高。 Abstract:Objective To compare the microbial differences of supragingival plaque between healthy gingiva group and gingivitis group of college students by high-throughput sequencing technology. Methods According to inclusion and exclusion criteria, 30 college students aged 18-25 with healthy gingiva and 33 with gingivitis were recruited from Kunming Medical University. Sequencing of 16S rDNA gene amplicon was performed using MiSeq system, and the diversity, structure and composition of supragingival plaque were compared between the two groups. Results A total of 87, 595 sequences were detected, including 14 phyla and 216 genera. Compared with the healthy gingiva group, the α diversity index in the gingivitis group was higher, and the difference was statistically significant （P < 0.001）. The result of similarity analysis （Anosim） and principal component analysis （PCA） showed that there were significant differences in the structure and composition of the microflora of supragingival plaque between the healthy gingiva group and the gingivitis group （P < 0.001）. The relative abundance of Prevotella, Porphyromonas and Bacillus in gingivitis group was higher, while the relative abundance of Actinomycetes in healthy gingiva group was higher. Conclusion There are significant differences in microbial community composition and structure of supragingival plaque between the healthy gingiva group and the gingivitis group of college students, and the diversity of the gingivitis group is higher. -
Key words:
- Gingivitis /
- High-throughput sequencing /
- Microbiome /
- College students
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图 1 牙龈健康组（NO）龈上菌斑物种丰度、多样性低于牙龈炎组（CO）
A：健康组与牙龈炎组间的物种总数的散点分布即丰度（richness）；B：Shannon指数比较图；***P < 0.001。
Figure 1. The species abundance and diversity of plaque in gingivitis group （NO） were lower than that in gingivitis group （CO）
图 2 基于OTU丰度的主成分分析（PCoA）
Figure 2. Principal Component Analysis （PCoA） based on OTU abundance
图 3 门水平健康组（NO）与牙龈炎组（CO）的物种相对丰度
该菌门在NO组中水平显著较高，*P < 0.05。
Figure 3. Relative species abundance of the healthy group （NO） and the gingivitis group （CO） at phylum level
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[1] 孟焕新. 牙周病学[M]. 第5版. 北京: 人民卫生出版社, 2020: 131. [2] Chambrone L,Wang H L,Romanos G E. Antimicrobial photodynamic therapy for the treatment of periodontitis and peri-implantitis:An American Academy of Periodontology best evidence review[J]. J Periodontol,2018,89(7):783-803. [3] Nazir M,Al-Ansari A,Al-Khalifa K,et al. Global prevalence of periodontal disease and lack of its surveillance[J]. Scientific World Journal,2020,20(20):214-216. [4] Bindakhil M,Sollecito T P,Stoopler E T. Severe gingival swelling and erythema[J]. Cutis,2020,105(6):19-21. [5] 王兴. 第四次全国口腔健康流行病学调查报告[M]. 北京: 人民卫生出版社, 2018: 106-107. [6] Murakami S,Mealey B L,Mariotti A,et al. Dental plaque-induced gingival conditions[J]. J Periodontol,2018,89(1):17-27. [7] Aghazada R,Marini L,Zeza B,et al. Experimental gingivitis in patients with and without altered passive eruption[J]. J Periodontol,2020,91(7):938-946. doi: 10.1002/JPER.19-0443 [8] Slatko B E,Gardner A F,Ausubel F M. Overview of next-generation sequencing technologies[J]. Curr Protoc Mol Biol,2018,122(1):59-64. [9] Gu W,Miller S,Chiu C Y. Clinical metagenomic next-generation sequencing for pathogen detection[J]. Annu Rev Pathol,2019,24(14):319-338. [10] Caton J G,Armitage G,Berglundh T,et al. A new classification scheme for periodontal and peri-implant diseases and conditions-introduction and key changes from the 1999 classification[J]. J Clin Periodontol,2018,45(20):1-8. [11] Daly S,Seong J,Newcombe R,Davies M,et al. A randomised clinical trial to determine the effect of a toothpaste containing enzymes and proteins on gum health over 3 months[J]. J Dent,2019,80(1):26-32. [12] Pedersen A M L,Darwish M,Nicholson J,et al. Gingival health status in individuals using different types of toothpaste[J]. J Dent,2019,80(1):13-18. [13] Abu Fanas S,Brigi C,Vanma S R,et al. The prevalence of novel periodontal pathogens and bacterial complexes in Stage Ⅱ generalized periodontitis based on 16S rRNA next generation sequencing[J]. J Appl Oral Sci,2021,17(29):e20200787. [14] Matsha T E,Prince Y,Davids S,et al. Oral microbiome signatures in diabetes mellitus and periodontal disease[J]. J Dent Res,2020,99(6):658-665. doi: 10.1177/0022034520913818 [15] Deng K,Ouyang X Y,Chu Y,et al. Subgingival microbiome of gingivitis in Chinese undergraduates[J]. Chin J Dent Res,2017,20(3):145-152. [16] Abusleme L,Hoare A,Hong B Y,et al. Microbial signatures of health,gingivitis,and periodontitis[J]. Periodontol,2000,2021,86(1):57-78. [17] Lopes M P,Cruz Á A,Xavier M T,et al. Prevotella intermedia and periodontitis are associated with severe asthma[J]. J Periodontol,2020,91(1):46-54. doi: 10.1002/JPER.19-0065 [18] Cui X,Liu J,Xiao W,et al. Subgingival microbiome in Chinese patients with generalized aggressive periodontitis compared to healthy controls[J]. Arch Oral Biol,2019,101(2):92-99. [19] Yang N Y,Zhang Q,Li J L,et al. Progression of periodontal inflammation in adolescents is associated with increased number of Porphyromonas gingivalis,Prevotella intermedia,Tannerella forsyth-ensis,and Fusobacterium nucleatum[J]. Paediatr Dent,2014,24(3):226-233. doi: 10.1111/ipd.12065 [20] Lenartova M,Tesinska B,Janatova T,et al. The oral microbiome in periodontal health[J]. Front Cell Infect Microbiol,2021,22(11):629-645. [21] Nowicki E M,Shroff R,Singleton J A,et al. Microbiota and metatranscriptome changes accompanying the onset of gingivitis[J]. mBio,2018,9(2):e1-e18. [22] Park O J,Yi H,Jeon J H,et al. Pyrose-quencing analysis of subgingival microbiota in distinct periodontal conditions[J]. Dent Res,2015,94(6):921-927. -
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